Long-term restricted feeding alters circadian expression and reduces the level of inflammatory and disease markers.
Bottom Line: However, it is not known whether RF can delay the occurrence of age-associated changes similar to CR.We found that circadian rhythmicity is more robust and is phase advanced in most of the genes and proteins tested under RF.Our results suggest that RF may share some benefits with those of CR.
Affiliation: Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.Show MeSH
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Mentions: Robust oscillation and a phase advance were also observed in most of the metabolical markers in both liver and jejunum under RF. As expected, under AL, Ampk mRNA oscillation peaked during the light phase, the time of inactivity, whereas under RF, it peaked before food availability. This is in agreement with the low energy levels when the animals were asleep under the AL regimen or devoid of food under the RF regimen. Under RF, when AMP levels increase, AMPK is activated, as indeed is demonstrated by the higher pAMPK/AMPK ratio (Fig. 4), to boost production of ATP and inhibit its usage. Similarly, the NAD+ histone deacetylase SIRT1 activity is up-regulated in response to changes in the energy status. SIRT1 activation promotes transcription of genes that mediate the metabolic response to stress or starvation, among which is PGC-1α (Fig. 10), as was reported . Indeed, PGC-1α involvement in the switch from glycolysis to gluconeogenesis under fasting conditions in the liver is well documented [79–81] and fits well with the temporal food restriction inherent to the RF model.
Affiliation: Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.