Keeping track of the growing number of biological functions of chitin and its interaction partners in biomedical research.
Bottom Line: In many studies, these proteins have been found to be involved in immune regulation and in mediating the degradation of chitinous external protective structures of invading pathogens.Finally, we examine the existing literature on zebrafish chitinases, and propose the use of zebrafish as a versatile model to complement the existing murine models.This could especially be of benefit to the exploration of the function of chitinases in infectious diseases using high-throughput approaches and pharmaceutical interventions.
Affiliation: Department of Molecular Biology and Genetics, Aarhus University, Aarhus C, Denmark Leiden University, Institute of Biology, Leiden, The Netherlands.Show MeSH
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Mentions: The exciting recent findings linking chitinases and CLPs to the cellular immune responses towards bacterial (Cozzarini et al. 2009; Dela Cruz et al. 2012; Tran et al. 2014) and fungal (Wagener et al. 2014) infections highlights the necessity for an amenable in vivo infection model. One such model for studies of disease progression and impact of genetic and pharmaceutical approaches could be the zebrafish. The optical clarity of the developing larvae makes it amenable to real-time evaluation of disease progression and high-throughput compound screening. These unique features allow for high-throughput, as well as detailed, microscopy and image-based analysis of disease progression and innate immune cell response patterns (see Figure 5) and (Brothers et al. 2013; Knox et al. 2014).Fig. 5.
Affiliation: Department of Molecular Biology and Genetics, Aarhus University, Aarhus C, Denmark Leiden University, Institute of Biology, Leiden, The Netherlands.