Limits...
Lactobacillus protects the integrity of intestinal epithelial barrier damaged by pathogenic bacteria.

Yu Q, Yuan L, Deng J, Yang Q - Front Cell Infect Microbiol (2015)

Bottom Line: Moreover, some Lactobacillus could inhibit the adhesion of the pathogens and protect the integrity of the cell junction and mucosal barrier.This research focused on the potential therapeutic effect of Lactobacillus fructosus (L. fructosus) C2 to attenuate ETEC K88 or S. typhimurium SL1344 induced changes to mucosal barrier.The results demonstrated that treatment of polarized Caco-2 cells with L. fructosus C2 reduced the permeation of dextran, and expression of IL-8, p-ERK, and p-JNK when cells were infected with pathogenic bacteria.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Nanjing Agricultural University Nanjing, China.

ABSTRACT
Pathogens invade intestinal mucosal barrier through phagocytosis of antigen presenting cells (dendritic cell, microfold cells), or through the invasion into the intestinal epithelial directly. Some pathogens could damage the cell junction between epithelial cells and use the paracellular pathway as an entrance to invade. Moreover, some Lactobacillus could inhibit the adhesion of the pathogens and protect the integrity of the cell junction and mucosal barrier. This research focused on the potential therapeutic effect of Lactobacillus fructosus (L. fructosus) C2 to attenuate ETEC K88 or S. typhimurium SL1344 induced changes to mucosal barrier. The results demonstrated that treatment of polarized Caco-2 cells with L. fructosus C2 reduced the permeation of dextran, and expression of IL-8, p-ERK, and p-JNK when cells were infected with pathogenic bacteria. The findings indicated that L. fructosus C2 exerted a protective effect against the damage to the integrity of Caco-2 cells by ETEC or S. typhimurium infection.

Show MeSH

Related in: MedlinePlus

Effects of L. fructosus C2 on IL-8 production. IL-8 production was measured by ELISA method after cells were treated with L. fructosus C2 (MOI 200:1) or pathogens (ETEC or S. typhimurium, MOI 20:1) simultaneously for 6 h. *P < 0.05, as determined by ANOVA. The data represent results from four independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4373387&req=5

Figure 2: Effects of L. fructosus C2 on IL-8 production. IL-8 production was measured by ELISA method after cells were treated with L. fructosus C2 (MOI 200:1) or pathogens (ETEC or S. typhimurium, MOI 20:1) simultaneously for 6 h. *P < 0.05, as determined by ANOVA. The data represent results from four independent experiments.

Mentions: The IL-8 expression was evaluated, when the Caco-2 cells were co-cultured with pathogens (ETEC K88 or S. typhimurium SL1344), L. fructosus C2 alone or simultaneously (Figure 2). Infection with ETEC K88 or S. typhimurium SL1344 caused a significant up-regulation of IL-8 expression compared to the untreated cells (P < 0.05). However, we found that treatment with L. fructosus C2 and ETEC K88 simultaneously inhibited the increase of IL-8 expression caused by ETEC alone significantly (P < 0.05). Compared to the S. typhimurium SL1344 infection, co-culture with L. fructosus C2 and S. typhimurium SL1344 could reduce the IL-8 secretion significantly, but still could not recover to the normal status.


Lactobacillus protects the integrity of intestinal epithelial barrier damaged by pathogenic bacteria.

Yu Q, Yuan L, Deng J, Yang Q - Front Cell Infect Microbiol (2015)

Effects of L. fructosus C2 on IL-8 production. IL-8 production was measured by ELISA method after cells were treated with L. fructosus C2 (MOI 200:1) or pathogens (ETEC or S. typhimurium, MOI 20:1) simultaneously for 6 h. *P < 0.05, as determined by ANOVA. The data represent results from four independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4373387&req=5

Figure 2: Effects of L. fructosus C2 on IL-8 production. IL-8 production was measured by ELISA method after cells were treated with L. fructosus C2 (MOI 200:1) or pathogens (ETEC or S. typhimurium, MOI 20:1) simultaneously for 6 h. *P < 0.05, as determined by ANOVA. The data represent results from four independent experiments.
Mentions: The IL-8 expression was evaluated, when the Caco-2 cells were co-cultured with pathogens (ETEC K88 or S. typhimurium SL1344), L. fructosus C2 alone or simultaneously (Figure 2). Infection with ETEC K88 or S. typhimurium SL1344 caused a significant up-regulation of IL-8 expression compared to the untreated cells (P < 0.05). However, we found that treatment with L. fructosus C2 and ETEC K88 simultaneously inhibited the increase of IL-8 expression caused by ETEC alone significantly (P < 0.05). Compared to the S. typhimurium SL1344 infection, co-culture with L. fructosus C2 and S. typhimurium SL1344 could reduce the IL-8 secretion significantly, but still could not recover to the normal status.

Bottom Line: Moreover, some Lactobacillus could inhibit the adhesion of the pathogens and protect the integrity of the cell junction and mucosal barrier.This research focused on the potential therapeutic effect of Lactobacillus fructosus (L. fructosus) C2 to attenuate ETEC K88 or S. typhimurium SL1344 induced changes to mucosal barrier.The results demonstrated that treatment of polarized Caco-2 cells with L. fructosus C2 reduced the permeation of dextran, and expression of IL-8, p-ERK, and p-JNK when cells were infected with pathogenic bacteria.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Nanjing Agricultural University Nanjing, China.

ABSTRACT
Pathogens invade intestinal mucosal barrier through phagocytosis of antigen presenting cells (dendritic cell, microfold cells), or through the invasion into the intestinal epithelial directly. Some pathogens could damage the cell junction between epithelial cells and use the paracellular pathway as an entrance to invade. Moreover, some Lactobacillus could inhibit the adhesion of the pathogens and protect the integrity of the cell junction and mucosal barrier. This research focused on the potential therapeutic effect of Lactobacillus fructosus (L. fructosus) C2 to attenuate ETEC K88 or S. typhimurium SL1344 induced changes to mucosal barrier. The results demonstrated that treatment of polarized Caco-2 cells with L. fructosus C2 reduced the permeation of dextran, and expression of IL-8, p-ERK, and p-JNK when cells were infected with pathogenic bacteria. The findings indicated that L. fructosus C2 exerted a protective effect against the damage to the integrity of Caco-2 cells by ETEC or S. typhimurium infection.

Show MeSH
Related in: MedlinePlus