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Neural circuits containing olfactory neurons are involved in the prepulse inhibition of the startle reflex in rats.

Niu H, He X, Zhou T, Shi X, Zhang Q, Zhang Z, Qiao Y, Xu F, Hu M - Front Behav Neurosci (2015)

Bottom Line: Our results demonstrated that blockage of olfactory sensory input could disturb olfactory behavior.In the function studies, we demonstrated that blockage of olfactory sensory input could impair the pre-pulse inhibition of the startle response following decreased c-Fos expression in relevant brain regions during the PPI responses.Thus, these data suggest that the olfactory system participates in the PPI regulating fields and plays a role in the pre-pulse inhibition of the startle response in rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics, Xuzhou Medical College Xuzhou, China ; The Institute of Audiology and Speech Science, Xuzhou Medical Collage Xuzhou, China.

ABSTRACT
Many neuropsychiatric disorders, such as schizophrenia, have been associated with olfactory dysfunction and abnormalities in the prepulse inhibition (PPI) response to a startle reflex. However, whether these two abnormalities could be related is unclear. The present investigations were designed to determine whether theblockage of olfactory sensory input by zinc sulfate infusion in the olfactory naris (0.5 ml, 0.17 M, ZnE) can disturb the PPI response. Furthermore, a bilateral microinjection of lidocaine/MK801 in the olfactory bulb (OB) was administered to examine whether the blockage of olfactory sensory input could impair the PPI response. To identify the neural projection between olfaction and PPI-related areas, trans-synaptic retrograde tracing with the recombinant pseudorabies virus (PRV) was used. Our results demonstrated that blockage of olfactory sensory input could disturb olfactory behavior. In the function studies, we demonstrated that blockage of olfactory sensory input could impair the pre-pulse inhibition of the startle response following decreased c-Fos expression in relevant brain regions during the PPI responses. Furthermore, similar and more robust findings indicated that blockage of olfactory sensory input by microinjection of lidocaine/MK801 in the OB could impair the PPI response. In the circuit-level studies, we demonstrated that trans-synaptic retrograde tracing with PRV exhibited a large portion of labeled neurons in several regions of the olfactory cortices from the pedunculopontine tegmental nucleus (PPTg). Thus, these data suggest that the olfactory system participates in the PPI regulating fields and plays a role in the pre-pulse inhibition of the startle response in rats.

No MeSH data available.


Related in: MedlinePlus

The distribution of PRV614-mRFP trans-synaptically labeled neurons in several regions of the olfactory cortices after 4 days of infection. (A) The distribution of trans-synaptically labeled neurons in the dorsal aspect of the anterior olfactory nucleus (DAON). (B) The distribution of trans-synaptically labeled neurons in the anterior piriform cortex (APc). (C) The distribution of trans-synaptically labeled neurons in the posterior piriform cortex (PPc). (D) The distribution of trans-synaptically labeled neurons in the lateral entorhinal cortex (LEnt). Scale bar = 200 µm.
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Figure 5: The distribution of PRV614-mRFP trans-synaptically labeled neurons in several regions of the olfactory cortices after 4 days of infection. (A) The distribution of trans-synaptically labeled neurons in the dorsal aspect of the anterior olfactory nucleus (DAON). (B) The distribution of trans-synaptically labeled neurons in the anterior piriform cortex (APc). (C) The distribution of trans-synaptically labeled neurons in the posterior piriform cortex (PPc). (D) The distribution of trans-synaptically labeled neurons in the lateral entorhinal cortex (LEnt). Scale bar = 200 µm.

Mentions: In addition to the regions known to regulate the PPI of the ASR, a large number of labeled neurons were distributed in several areas of the primary olfactory cortex, including the anterior olfactory nucleus (AON), the piriform cortex (Pcx) and the lateral entorhinal cortex (LEnt; Figure 5). The majority of the labeled neurons in the AON were distributed in the dorsal aspect (Figure 6A). Most labeled neurons in the Pcx were identified in layer II of the Pcx and were distributed from the anterior (APc) to posterior (PPc) aspects. The neurons in layer III of the Pcx were also labeled to some extent (Figures 5B,C). The neurons in the LEnt were prominently labeled in both layers II and III (Figure 5D).


Neural circuits containing olfactory neurons are involved in the prepulse inhibition of the startle reflex in rats.

Niu H, He X, Zhou T, Shi X, Zhang Q, Zhang Z, Qiao Y, Xu F, Hu M - Front Behav Neurosci (2015)

The distribution of PRV614-mRFP trans-synaptically labeled neurons in several regions of the olfactory cortices after 4 days of infection. (A) The distribution of trans-synaptically labeled neurons in the dorsal aspect of the anterior olfactory nucleus (DAON). (B) The distribution of trans-synaptically labeled neurons in the anterior piriform cortex (APc). (C) The distribution of trans-synaptically labeled neurons in the posterior piriform cortex (PPc). (D) The distribution of trans-synaptically labeled neurons in the lateral entorhinal cortex (LEnt). Scale bar = 200 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4373374&req=5

Figure 5: The distribution of PRV614-mRFP trans-synaptically labeled neurons in several regions of the olfactory cortices after 4 days of infection. (A) The distribution of trans-synaptically labeled neurons in the dorsal aspect of the anterior olfactory nucleus (DAON). (B) The distribution of trans-synaptically labeled neurons in the anterior piriform cortex (APc). (C) The distribution of trans-synaptically labeled neurons in the posterior piriform cortex (PPc). (D) The distribution of trans-synaptically labeled neurons in the lateral entorhinal cortex (LEnt). Scale bar = 200 µm.
Mentions: In addition to the regions known to regulate the PPI of the ASR, a large number of labeled neurons were distributed in several areas of the primary olfactory cortex, including the anterior olfactory nucleus (AON), the piriform cortex (Pcx) and the lateral entorhinal cortex (LEnt; Figure 5). The majority of the labeled neurons in the AON were distributed in the dorsal aspect (Figure 6A). Most labeled neurons in the Pcx were identified in layer II of the Pcx and were distributed from the anterior (APc) to posterior (PPc) aspects. The neurons in layer III of the Pcx were also labeled to some extent (Figures 5B,C). The neurons in the LEnt were prominently labeled in both layers II and III (Figure 5D).

Bottom Line: Our results demonstrated that blockage of olfactory sensory input could disturb olfactory behavior.In the function studies, we demonstrated that blockage of olfactory sensory input could impair the pre-pulse inhibition of the startle response following decreased c-Fos expression in relevant brain regions during the PPI responses.Thus, these data suggest that the olfactory system participates in the PPI regulating fields and plays a role in the pre-pulse inhibition of the startle response in rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics, Xuzhou Medical College Xuzhou, China ; The Institute of Audiology and Speech Science, Xuzhou Medical Collage Xuzhou, China.

ABSTRACT
Many neuropsychiatric disorders, such as schizophrenia, have been associated with olfactory dysfunction and abnormalities in the prepulse inhibition (PPI) response to a startle reflex. However, whether these two abnormalities could be related is unclear. The present investigations were designed to determine whether theblockage of olfactory sensory input by zinc sulfate infusion in the olfactory naris (0.5 ml, 0.17 M, ZnE) can disturb the PPI response. Furthermore, a bilateral microinjection of lidocaine/MK801 in the olfactory bulb (OB) was administered to examine whether the blockage of olfactory sensory input could impair the PPI response. To identify the neural projection between olfaction and PPI-related areas, trans-synaptic retrograde tracing with the recombinant pseudorabies virus (PRV) was used. Our results demonstrated that blockage of olfactory sensory input could disturb olfactory behavior. In the function studies, we demonstrated that blockage of olfactory sensory input could impair the pre-pulse inhibition of the startle response following decreased c-Fos expression in relevant brain regions during the PPI responses. Furthermore, similar and more robust findings indicated that blockage of olfactory sensory input by microinjection of lidocaine/MK801 in the OB could impair the PPI response. In the circuit-level studies, we demonstrated that trans-synaptic retrograde tracing with PRV exhibited a large portion of labeled neurons in several regions of the olfactory cortices from the pedunculopontine tegmental nucleus (PPTg). Thus, these data suggest that the olfactory system participates in the PPI regulating fields and plays a role in the pre-pulse inhibition of the startle response in rats.

No MeSH data available.


Related in: MedlinePlus