Circulating stem cell vary with NYHA stage in heart failure patients.
Bottom Line: Compared with healthy individuals, MSC, and in particular the sub-classes CD45(-) CD34(-) CD90(+) , CD45(-) CD34(-) CD105(+) and CD45(-) CD34(-) CXCR4(+) were significantly enhanced in NYHA class IV patients (16.8-, 6.4- and 2.7-fold, respectively).Level of CD45(-) CD34(-) CD90(+) CXCR4(+) cells progressively increased from class II to class IV (fold increases compared with controls: 8.5, 12 and 21.5, respectively).Both the entity and kinetic of this process varied in distinct cell subsets.
Affiliation: University of Ferrara and Cardiovascular Research Center, Salvatore Maugeri Foundation, IRCCS, Lumezzane, Italy. firstname.lastname@example.orgShow MeSH
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Mentions: Table 2 summarizes the Abs combination utilized to identify the different subpopulation of each class of BMSC. Figure 1 shows a typical example of analytical gates used to count total numbers and subsets of circulating stem cells. Putative MSCs were identified as CD45−CD34− cells, expressing either CD90 or CD105 ; HSCs as CD45+ and CD34+ cells co-expressing either CD90 or CD105 ; EPCs, a very heterogeneous group of cells, were characterized as CD45− with or without the surface markers CD133 and CD144 [14–16]. TCSCs were identified as CD34+CD45+ or CD45− cells co-expressing the CXCR4 receptor . In view of the role of SDF-1α-CXCR4 interaction in homing, repopulation and recruitment of human stem cells , the expression of CXCR4 receptor was also evaluated in all groups except for the EPCs, because this was already evaluated in the CD45−CD34+ component of the TCSCs. Because of the role of PDGF-BB in MSC recruitment, we evaluated the axis PDGF-BB/PDGFR as well .
Affiliation: University of Ferrara and Cardiovascular Research Center, Salvatore Maugeri Foundation, IRCCS, Lumezzane, Italy. email@example.com