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Cortical network from human embryonic stem cells.

Nat R - J. Cell. Mol. Med. (2011)

Bottom Line: The connection of embryonic stem cell technology and developmental biology provides valuable tools to decipher the mechanisms underlying human brain development and diseases, especially among neuronal populations, that are not readily available in primary cultures.It is obviously the case of neurons forming the human cerebral cortex.In the images that are presented, the neurons were generated in vitro from human embryonic stem cells via forebrain-like progenitors.

View Article: PubMed Central - PubMed

Affiliation: Department of Cellular and Molecular Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania. Irina-Roxana.Nat@i-med.ac.at

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Neuronal network of glutamatergic pyramidal-like neurons from hES cells. Human neurons derived from ES cells after 32 days of differentiation in vitro: they formed an extensive network, where the majority of cells are pyramidal-like neurons (A) expressing the neurotransmitter glutamate (B); staining for the neuronal marker tau (A—yellow) and glutamate (B—green); blue Hoechst 33342 nuclear staining. Scale bar: 20 μm.
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fig02: Neuronal network of glutamatergic pyramidal-like neurons from hES cells. Human neurons derived from ES cells after 32 days of differentiation in vitro: they formed an extensive network, where the majority of cells are pyramidal-like neurons (A) expressing the neurotransmitter glutamate (B); staining for the neuronal marker tau (A—yellow) and glutamate (B—green); blue Hoechst 33342 nuclear staining. Scale bar: 20 μm.

Mentions: The hES cell derived neurons were maintained in culture for prolonged time, with optimized culture conditions, and formed spectacular networks (Fig. 2A), where the majority acquired a glutamatergic phenotype (Fig. 2B). They presented pyramidal like-morphologies (Fig. 3A) and, like a novel aspect of hES cell derived neuronal morphology, dendritic spines with specific cortical morphologies could be identified in vitro (Fig. 3A, B), as was recently shown for the mES cell derived cortical neurons [8]. The subtype-related cortical neuronal specification and the functional characterization in vitro are under investigation.


Cortical network from human embryonic stem cells.

Nat R - J. Cell. Mol. Med. (2011)

Neuronal network of glutamatergic pyramidal-like neurons from hES cells. Human neurons derived from ES cells after 32 days of differentiation in vitro: they formed an extensive network, where the majority of cells are pyramidal-like neurons (A) expressing the neurotransmitter glutamate (B); staining for the neuronal marker tau (A—yellow) and glutamate (B—green); blue Hoechst 33342 nuclear staining. Scale bar: 20 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373340&req=5

fig02: Neuronal network of glutamatergic pyramidal-like neurons from hES cells. Human neurons derived from ES cells after 32 days of differentiation in vitro: they formed an extensive network, where the majority of cells are pyramidal-like neurons (A) expressing the neurotransmitter glutamate (B); staining for the neuronal marker tau (A—yellow) and glutamate (B—green); blue Hoechst 33342 nuclear staining. Scale bar: 20 μm.
Mentions: The hES cell derived neurons were maintained in culture for prolonged time, with optimized culture conditions, and formed spectacular networks (Fig. 2A), where the majority acquired a glutamatergic phenotype (Fig. 2B). They presented pyramidal like-morphologies (Fig. 3A) and, like a novel aspect of hES cell derived neuronal morphology, dendritic spines with specific cortical morphologies could be identified in vitro (Fig. 3A, B), as was recently shown for the mES cell derived cortical neurons [8]. The subtype-related cortical neuronal specification and the functional characterization in vitro are under investigation.

Bottom Line: The connection of embryonic stem cell technology and developmental biology provides valuable tools to decipher the mechanisms underlying human brain development and diseases, especially among neuronal populations, that are not readily available in primary cultures.It is obviously the case of neurons forming the human cerebral cortex.In the images that are presented, the neurons were generated in vitro from human embryonic stem cells via forebrain-like progenitors.

View Article: PubMed Central - PubMed

Affiliation: Department of Cellular and Molecular Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania. Irina-Roxana.Nat@i-med.ac.at

Show MeSH