Cortical network from human embryonic stem cells.
Bottom Line: The connection of embryonic stem cell technology and developmental biology provides valuable tools to decipher the mechanisms underlying human brain development and diseases, especially among neuronal populations, that are not readily available in primary cultures.It is obviously the case of neurons forming the human cerebral cortex.In the images that are presented, the neurons were generated in vitro from human embryonic stem cells via forebrain-like progenitors.
Affiliation: Department of Cellular and Molecular Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania. Irina-Roxana.Nat@i-med.ac.atShow MeSH
Mentions: First, neuroepithelial cells were induced from hES cells, in chemical-defined cell culture conditions, without morphogens, and they acquired an anterior phenotype [5–7]. They differentiated into neural progenitor cells expressing the forebrain-related transcription factor FOXG1 (Fig. 1A) and the forebrain membranar marker Forse1 (Fig. 1B), and started to generate neurons (Fig. 1A, B).
Affiliation: Department of Cellular and Molecular Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania. Irina-Roxana.Nat@i-med.ac.at