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Promoter hypomethylation up-regulates CD147 expression through increasing Sp1 binding and associates with poor prognosis in human hepatocellular carcinoma.

Kong LM, Liao CG, Chen L, Yang HS, Zhang SH, Zhang Z, Bian HJ, Xing JL, Chen ZN - J. Cell. Mol. Med. (2010)

Bottom Line: Significantly higher expression of CD147 and significantly lower promoter methylation level were observed in HCC cell lines compared to normal cell lines and tissues control.Moreover, HCC patients with unmethylated CD147 promoter had a significantly higher recurrence rate (88.1%versus 58.3%; P < 0.05) and death rate (83.3%versus 50.0%; P < 0.05) than patients with methylated CD147 promoter.In conclusions, promoter hypomethylation up-regulates CD147 expression primarily through increasing Sp1 binding and associates with poor prognosis in HCC patients.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Cancer Biology, Cell Engineering Research Centre & Department of Cell Biology, Fourth Military Medical University, Xi'an, China.

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Up-expression of CD147 in HCC cell lines. (A) mRNA expressions of CD147 gene were detected by real-time quantitative RT-PCR in HCC cell lines, normal cell lines and normal liver tissues. GAPDH gene was used as an internal control. (B) CD147 protein expression was determined by Western blot with tubulin as an internal control.
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fig01: Up-expression of CD147 in HCC cell lines. (A) mRNA expressions of CD147 gene were detected by real-time quantitative RT-PCR in HCC cell lines, normal cell lines and normal liver tissues. GAPDH gene was used as an internal control. (B) CD147 protein expression was determined by Western blot with tubulin as an internal control.

Mentions: The expression levels of CD147 RNA and protein were evaluated in three human normal liver tissues, normal liver cell lines and HCC cell lines. Real-time quantitative RT-PCR analysis showed that there were significantly higher mRNA expression of CD147 in all HCC cell lines (HepG2, BEL-7402, FHCC-98, SMMC-7721, HCC-9724 and HCC-9204), compared with the normal cell lines (QZG, QSG-7701 and HL-7702) and normal tissues (NL1, NL2 and NL3) (Fig. 1A). Similar results were also observed in Western blot analysis, indicating that the protein expression of CD147 was dramatically higher in HCC cell lines (Fig. 1B).


Promoter hypomethylation up-regulates CD147 expression through increasing Sp1 binding and associates with poor prognosis in human hepatocellular carcinoma.

Kong LM, Liao CG, Chen L, Yang HS, Zhang SH, Zhang Z, Bian HJ, Xing JL, Chen ZN - J. Cell. Mol. Med. (2010)

Up-expression of CD147 in HCC cell lines. (A) mRNA expressions of CD147 gene were detected by real-time quantitative RT-PCR in HCC cell lines, normal cell lines and normal liver tissues. GAPDH gene was used as an internal control. (B) CD147 protein expression was determined by Western blot with tubulin as an internal control.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373337&req=5

fig01: Up-expression of CD147 in HCC cell lines. (A) mRNA expressions of CD147 gene were detected by real-time quantitative RT-PCR in HCC cell lines, normal cell lines and normal liver tissues. GAPDH gene was used as an internal control. (B) CD147 protein expression was determined by Western blot with tubulin as an internal control.
Mentions: The expression levels of CD147 RNA and protein were evaluated in three human normal liver tissues, normal liver cell lines and HCC cell lines. Real-time quantitative RT-PCR analysis showed that there were significantly higher mRNA expression of CD147 in all HCC cell lines (HepG2, BEL-7402, FHCC-98, SMMC-7721, HCC-9724 and HCC-9204), compared with the normal cell lines (QZG, QSG-7701 and HL-7702) and normal tissues (NL1, NL2 and NL3) (Fig. 1A). Similar results were also observed in Western blot analysis, indicating that the protein expression of CD147 was dramatically higher in HCC cell lines (Fig. 1B).

Bottom Line: Significantly higher expression of CD147 and significantly lower promoter methylation level were observed in HCC cell lines compared to normal cell lines and tissues control.Moreover, HCC patients with unmethylated CD147 promoter had a significantly higher recurrence rate (88.1%versus 58.3%; P < 0.05) and death rate (83.3%versus 50.0%; P < 0.05) than patients with methylated CD147 promoter.In conclusions, promoter hypomethylation up-regulates CD147 expression primarily through increasing Sp1 binding and associates with poor prognosis in HCC patients.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Cancer Biology, Cell Engineering Research Centre & Department of Cell Biology, Fourth Military Medical University, Xi'an, China.

Show MeSH
Related in: MedlinePlus