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Identification of telocytes in skeletal muscle interstitium: implication for muscle regeneration.

Popescu LM, Manole E, Serboiu CS, Manole CG, Suciu LC, Gherghiceanu M, Popescu BO - J. Cell. Mol. Med. (2011)

Bottom Line: The same phenotypic profile was demonstrated in cell cultures.We also described non-satellite (resident) progenitor cell niche.In culture, TCs (but not satellite cells) emerge from muscle explants and form networks suggesting a key role in muscle regeneration and repair, at least after trauma.

View Article: PubMed Central - PubMed

Affiliation: Department of Cellular and Molecular Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania. LMP@jcmm.org

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Human skeletal muscle, immunohistochemistry with HRP conjugated antibodies on cryosections (A, B, E, H) and immunofluorescence-confocal microscopy (C, D). TCs are located within interstitium, and express c-kit (A–D), caveolin-1 (E), vimentin (F) and VEGF (G). TCs were also revealed by methylene blue vital staining (H). In C and D, TCs are identified by c-kit expression (green), the basal lamina by laminin expression (red) and nuclei are stained with DAPI (blue). Original magnification 1000×.
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fig09: Human skeletal muscle, immunohistochemistry with HRP conjugated antibodies on cryosections (A, B, E, H) and immunofluorescence-confocal microscopy (C, D). TCs are located within interstitium, and express c-kit (A–D), caveolin-1 (E), vimentin (F) and VEGF (G). TCs were also revealed by methylene blue vital staining (H). In C and D, TCs are identified by c-kit expression (green), the basal lamina by laminin expression (red) and nuclei are stained with DAPI (blue). Original magnification 1000×.

Mentions: In skeletal muscle interstitium, TCs became apparent with a short exposure of cryosections to methylene blue staining (Fig. 9), as we reported for other tissues. Immunostaining for proteins typically expressed by TCs (c-kit, caveolin-1, vimentin and VEGF) revealed individual interstitial cells, with long and thin cell processes in a network distribution (Tps), both in human (Fig. 9) and rat (data not shown) muscle specimens. The location of cells with TC phenotype was restricted to perimysium and endomysium, their cell prolongations being in contact with blood vessels and nerve endings (Fig. 9), as seen in more detail with TEM. No obvious differences of immunostaining pattern or TC distribution were found in human and rat skeletal muscles examined.


Identification of telocytes in skeletal muscle interstitium: implication for muscle regeneration.

Popescu LM, Manole E, Serboiu CS, Manole CG, Suciu LC, Gherghiceanu M, Popescu BO - J. Cell. Mol. Med. (2011)

Human skeletal muscle, immunohistochemistry with HRP conjugated antibodies on cryosections (A, B, E, H) and immunofluorescence-confocal microscopy (C, D). TCs are located within interstitium, and express c-kit (A–D), caveolin-1 (E), vimentin (F) and VEGF (G). TCs were also revealed by methylene blue vital staining (H). In C and D, TCs are identified by c-kit expression (green), the basal lamina by laminin expression (red) and nuclei are stained with DAPI (blue). Original magnification 1000×.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373336&req=5

fig09: Human skeletal muscle, immunohistochemistry with HRP conjugated antibodies on cryosections (A, B, E, H) and immunofluorescence-confocal microscopy (C, D). TCs are located within interstitium, and express c-kit (A–D), caveolin-1 (E), vimentin (F) and VEGF (G). TCs were also revealed by methylene blue vital staining (H). In C and D, TCs are identified by c-kit expression (green), the basal lamina by laminin expression (red) and nuclei are stained with DAPI (blue). Original magnification 1000×.
Mentions: In skeletal muscle interstitium, TCs became apparent with a short exposure of cryosections to methylene blue staining (Fig. 9), as we reported for other tissues. Immunostaining for proteins typically expressed by TCs (c-kit, caveolin-1, vimentin and VEGF) revealed individual interstitial cells, with long and thin cell processes in a network distribution (Tps), both in human (Fig. 9) and rat (data not shown) muscle specimens. The location of cells with TC phenotype was restricted to perimysium and endomysium, their cell prolongations being in contact with blood vessels and nerve endings (Fig. 9), as seen in more detail with TEM. No obvious differences of immunostaining pattern or TC distribution were found in human and rat skeletal muscles examined.

Bottom Line: The same phenotypic profile was demonstrated in cell cultures.We also described non-satellite (resident) progenitor cell niche.In culture, TCs (but not satellite cells) emerge from muscle explants and form networks suggesting a key role in muscle regeneration and repair, at least after trauma.

View Article: PubMed Central - PubMed

Affiliation: Department of Cellular and Molecular Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania. LMP@jcmm.org

Show MeSH
Related in: MedlinePlus