Identification of telocytes in skeletal muscle interstitium: implication for muscle regeneration.
Bottom Line: The same phenotypic profile was demonstrated in cell cultures.We also described non-satellite (resident) progenitor cell niche.In culture, TCs (but not satellite cells) emerge from muscle explants and form networks suggesting a key role in muscle regeneration and repair, at least after trauma.
Affiliation: Department of Cellular and Molecular Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania. LMP@jcmm.orgShow MeSH
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Mentions: In skeletal muscle interstitium, TCs became apparent with a short exposure of cryosections to methylene blue staining (Fig. 9), as we reported for other tissues. Immunostaining for proteins typically expressed by TCs (c-kit, caveolin-1, vimentin and VEGF) revealed individual interstitial cells, with long and thin cell processes in a network distribution (Tps), both in human (Fig. 9) and rat (data not shown) muscle specimens. The location of cells with TC phenotype was restricted to perimysium and endomysium, their cell prolongations being in contact with blood vessels and nerve endings (Fig. 9), as seen in more detail with TEM. No obvious differences of immunostaining pattern or TC distribution were found in human and rat skeletal muscles examined.
Affiliation: Department of Cellular and Molecular Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania. LMP@jcmm.org