Role of aquaporin 5 in antigen-induced airway inflammation and mucous hyperproduction in mice.
Bottom Line: The present study firstly found the overexpression of AQP5 in the airway epithelium and submucosal glands of asthmatics.Furthermore, we aimed at evaluating the role of AQP5 in airway inflammation and mucous hyperproductions during chronic allergic responses to house dust mite (HDM).Thus, our results implicate involvement of AQP5 in the development of airway inflammation and mucous hyperproduction during chronic asthma.
Affiliation: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.Show MeSH
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Mentions: Figure 5 demonstrates that more cells with positive staining of MUC5AC (Fig. 5A) and MUC5B (Fig. 5B) were observed in the small airway of WT mice after chronic exposure to HDM, as compared with AQP5 KO animals. The number of MUC5AC (Fig. 6A) – and MUC5B (Fig. 6B) – positive epithelial cells in the airway was significantly higher in WT mice as compared with in AQP5 KO mice after chronic HDM challenge (P < 0.01 and 0.05, respectively) and in animals challenged with BPS (P < 0.01, respectively). BAL levels of MUC5AC proteins were significantly increased in HDM-challenged WT (P < 0.01) and AQP5 KO animals (P < 0.05), respectively, as shown in Figure 7A. AQP5 KO mice exhibited a significant decrease about 51.7% in BAL levels of MUC5AC after chronic HDM challenge as compared with WT mice (P < 0.05, Fig. 7A). To confirm the expression of MUC5AC and MUC5B in the lung tissue, mRNA levels of MUC5AC and MUC5B were assessed by quantitative real-time PCR. There was a significant increase in gene expression of MUC5AC (Fig. 7B) and MUC5B (Fig. 7C) in the lung tissue of WT (P < 0.010 and AQP5 KO animals (P < 0.05) challenged with HDM, as compared with those with PBS, respectively. The expression of both MUC5AC and MUC5B in AQP5 KO mouse lung tissue was significantly lower than that in WT mice after HDM challenge.
Affiliation: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.