Role of aquaporin 5 in antigen-induced airway inflammation and mucous hyperproduction in mice.
Bottom Line: The aquaporin (AQP)-5 may be involved in the process due to its contribution to the volume of liquid secreted from the airways.The present study firstly found the overexpression of AQP5 in the airway epithelium and submucosal glands of asthmatics.Furthermore, we aimed at evaluating the role of AQP5 in airway inflammation and mucous hyperproductions during chronic allergic responses to house dust mite (HDM).
Affiliation: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.Show MeSH
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Mentions: Administration of HDM for 5 weeks to C57BL/6 WT mice led to a significant increase in the amount of pulmonary oedema and epithelial hypertrophy in the terminal airway and leucocyte infiltration (Fig. 2B), although not in AQP5 KO animals (Fig. 2D). The total scores of histopathology in HDM-treated AQP5 WT mice were significantly higher than those in PBS-treated animals (Table 1), whereas AQP5 KO mice had significantly less inflammation at the airway as compared with AQP5 WT mice after HDM exposure. To determine whether AQP5 KO mice had reduced airway eosinophilia, we analysed the cell number in the peribronchial and perivascular tissues after HDM exposure. The percentage of airway eosinophils was significantly increased in WT mice with HDM challenge for 5 weeks as compared with those with PBS. HDM-treated AQP5 KO mice had significantly fewer eosinophils as compared with WT animals (Table 1). There was no significant difference of neutrophil numbers in the lung tissue between WT and AQP5 KO mice (data not shown).
Affiliation: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.