The carbonyl scavenger carnosine ameliorates dyslipidaemia and renal function in Zucker obese rats.
Bottom Line: Several indices of oxidative/carbonyl stress were also measured in plasma, urine and renal tissue.We found that both L- and D-CAR greatly reduced obese-related diseases in obese Zucker rat, by significantly restraining the development of dyslipidaemia, hypertension and renal injury, as demonstrated by both urinary parameters and electron microscopy examinations of renal tissue.Because the protective effect elicited by L- and D-CAR was almost superimposable, we conclude that the pharmacological action of L-CAR is not due to a pro-histaminic effect (D-CAR is not a precursor of histidine, since it is stable to peptidic hydrolysis), and prompted us to propose that some of the biological effects can be mediated by a direct carbonyl quenching mechanism.
Affiliation: Department of Pharmaceutical Sciences Pietro Pratesi, Università degli Studi di Milano, Milan, Italy. email@example.comShow MeSH
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Mentions: At the ultrastructural level, the increase in GBM thickness in ZK rats compared to LN animals (5.8 ± 0.6 nm versus 3.9 ± 0.5 nm; P < 0.01) is another important morphologic modification in renal disease linked to metabolic syndrome, already reported in Zucker obese rats  and in FVBdb/db diabetic mice . Although measurements were done on only four animals for each group, the results reported in Figure 6D clearly indicate the ability of both the treatments to prevent GBM alteration, with D-CAR being able to maintain the GBM thickness in the range of the LN controls (3.75 ± 0.8 nm). Furthermore, in treated rats, glomeruli foot processes of podocytes show normal appearance whereas in ZK they are swollen, as clearly shown in Figure 7A–D.
Affiliation: Department of Pharmaceutical Sciences Pietro Pratesi, Università degli Studi di Milano, Milan, Italy. firstname.lastname@example.org