The carbonyl scavenger carnosine ameliorates dyslipidaemia and renal function in Zucker obese rats.
Bottom Line: Several indices of oxidative/carbonyl stress were also measured in plasma, urine and renal tissue.We found that both L- and D-CAR greatly reduced obese-related diseases in obese Zucker rat, by significantly restraining the development of dyslipidaemia, hypertension and renal injury, as demonstrated by both urinary parameters and electron microscopy examinations of renal tissue.Because the protective effect elicited by L- and D-CAR was almost superimposable, we conclude that the pharmacological action of L-CAR is not due to a pro-histaminic effect (D-CAR is not a precursor of histidine, since it is stable to peptidic hydrolysis), and prompted us to propose that some of the biological effects can be mediated by a direct carbonyl quenching mechanism.
Affiliation: Department of Pharmaceutical Sciences Pietro Pratesi, Università degli Studi di Milano, Milan, Italy. email@example.comShow MeSH
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Mentions: The kinetics of the enzymatic hydrolysis of L-CAR and D-CAR in human serum was followed over 24 hrs, and the results are shown in Figure 2A. The resistance of the two enantiomers to serum carnosinase was significantly different: although L-CAR rapidly disappeared in human serum at the first time-point (60 min.), D-CAR remained unchanged until 6 hrs incubation, and decreased by only 6% after 24 hrs of incubation. It should be noted that L-CAR is rapidly hydrolized in human serum within 5 min. of incubation, as recently reported .
Affiliation: Department of Pharmaceutical Sciences Pietro Pratesi, Università degli Studi di Milano, Milan, Italy. firstname.lastname@example.org