Intracardiac injection of matrigel induces stem cell recruitment and improves cardiac functions in a rat myocardial infarction model.
Bottom Line: Compared to the MI control group (MI-PBS), matrigel significantly improved left ventricular function (n= 11, P < 0.05) assessed by pressure-volume loops after 4 weeks.The infarct wall thickness of left ventricle is significantly higher (P < 0.01) in MI-M (0.72 ± 0.02 mm, n = 10) compared with MI-PBS (0.62 ± 0.02 mm, n = 10).MI-M hearts exhibited higher capillary density (border 130.8 ± 4.7 versus 115.4 ± 6.0, P < 0.05; vessels per high-power field [HPF; 400×], n = 6) than MI-PBS hearts. c-Kit(+) stem cells (38.3 ± 5.3 versus 25.7 ± 1.5 c-Kit(+) cells per HPF [630×], n = 5, P < 0.05) and CD34(+) cells (13.0 ± 1.51 versus 5.6 ± 0.68 CD34(+) cells per HPF [630×], n = 5, P < 0.01) were significantly more numerous in MI-M than in MI-PBS in the infarcted hearts (n = 5, P < 0.05).
Affiliation: Reference- and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany.Show MeSH
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Mentions: To examine whether local matrigel delivery can home c-Kit+ and CD34+ cells to infracted myocardial zone, c-Kit+ and CD34+ cell number in the infarct zone of heart were assessed by immunostaining at 4 weeks after MI (Figs 4 and 5). There was a significant increase in the number of c-Kit+ cells in the MI-M group compared to the MI-PBS group (38.3 ± 5.3 versus 25.7 ± 1.5 c-Kit+ cells per HPF [630×], n= 5, P < 0.05, Fig. 4D). Similarly, the number of CD34+ was also significantly increased compared with MI-PBS (13.0 ± 1.51 versus 5.6 ± 0.67 CD34+ cells per HPF [630×], n= 5, P < 0.01, Fig. 5D). The c-Kit+ cells can seldom be seen in sham-operated rat. These observations therefore revealed that matrigel may enhance stem cells recruitment after MI.
Affiliation: Reference- and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany.