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Intracardiac injection of matrigel induces stem cell recruitment and improves cardiac functions in a rat myocardial infarction model.

Ou L, Li W, Zhang Y, Wang W, Liu J, Sorg H, Furlani D, Gäbel R, Mark P, Klopsch C, Wang L, Lützow K, Lendlein A, Wagner K, Klee D, Liebold A, Li RK, Kong D, Steinhoff G, Ma N - J. Cell. Mol. Med. (2010)

Bottom Line: Compared to the MI control group (MI-PBS), matrigel significantly improved left ventricular function (n= 11, P < 0.05) assessed by pressure-volume loops after 4 weeks.The infarct wall thickness of left ventricle is significantly higher (P < 0.01) in MI-M (0.72 ± 0.02 mm, n = 10) compared with MI-PBS (0.62 ± 0.02 mm, n = 10).MI-M hearts exhibited higher capillary density (border 130.8 ± 4.7 versus 115.4 ± 6.0, P < 0.05; vessels per high-power field [HPF; 400×], n = 6) than MI-PBS hearts. c-Kit(+) stem cells (38.3 ± 5.3 versus 25.7 ± 1.5 c-Kit(+) cells per HPF [630×], n = 5, P < 0.05) and CD34(+) cells (13.0 ± 1.51 versus 5.6 ± 0.68 CD34(+) cells per HPF [630×], n = 5, P < 0.01) were significantly more numerous in MI-M than in MI-PBS in the infarcted hearts (n = 5, P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Reference- and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany.

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Local matrigel injection increases the myocardial homing of c-Kit+ cells. Representative immunostaining for c-Kit in the three groups of animals. c-Kit+ cells (green) were identified in infarct border zone at 4 weeks after treatment. Red, TOPRO3 in nuclei. Yellow is shown after green [(A), sham, (B), MI-PBS, (C–F), MI-M]. (D) The number of c-Kit+ cells per HPF in MI-M hearts (n= 5) was significantly higher than in MI hearts (n= 5), and c-Kit+ cells can seldom be seen in sham animals (n= 5). *P < 0.01. (A–C) scale bars = 250 μm; (D–F) scale bars = 25 μm.
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fig04: Local matrigel injection increases the myocardial homing of c-Kit+ cells. Representative immunostaining for c-Kit in the three groups of animals. c-Kit+ cells (green) were identified in infarct border zone at 4 weeks after treatment. Red, TOPRO3 in nuclei. Yellow is shown after green [(A), sham, (B), MI-PBS, (C–F), MI-M]. (D) The number of c-Kit+ cells per HPF in MI-M hearts (n= 5) was significantly higher than in MI hearts (n= 5), and c-Kit+ cells can seldom be seen in sham animals (n= 5). *P < 0.01. (A–C) scale bars = 250 μm; (D–F) scale bars = 25 μm.

Mentions: To examine whether local matrigel delivery can home c-Kit+ and CD34+ cells to infracted myocardial zone, c-Kit+ and CD34+ cell number in the infarct zone of heart were assessed by immunostaining at 4 weeks after MI (Figs 4 and 5). There was a significant increase in the number of c-Kit+ cells in the MI-M group compared to the MI-PBS group (38.3 ± 5.3 versus 25.7 ± 1.5 c-Kit+ cells per HPF [630×], n= 5, P < 0.05, Fig. 4D). Similarly, the number of CD34+ was also significantly increased compared with MI-PBS (13.0 ± 1.51 versus 5.6 ± 0.67 CD34+ cells per HPF [630×], n= 5, P < 0.01, Fig. 5D). The c-Kit+ cells can seldom be seen in sham-operated rat. These observations therefore revealed that matrigel may enhance stem cells recruitment after MI.


Intracardiac injection of matrigel induces stem cell recruitment and improves cardiac functions in a rat myocardial infarction model.

Ou L, Li W, Zhang Y, Wang W, Liu J, Sorg H, Furlani D, Gäbel R, Mark P, Klopsch C, Wang L, Lützow K, Lendlein A, Wagner K, Klee D, Liebold A, Li RK, Kong D, Steinhoff G, Ma N - J. Cell. Mol. Med. (2010)

Local matrigel injection increases the myocardial homing of c-Kit+ cells. Representative immunostaining for c-Kit in the three groups of animals. c-Kit+ cells (green) were identified in infarct border zone at 4 weeks after treatment. Red, TOPRO3 in nuclei. Yellow is shown after green [(A), sham, (B), MI-PBS, (C–F), MI-M]. (D) The number of c-Kit+ cells per HPF in MI-M hearts (n= 5) was significantly higher than in MI hearts (n= 5), and c-Kit+ cells can seldom be seen in sham animals (n= 5). *P < 0.01. (A–C) scale bars = 250 μm; (D–F) scale bars = 25 μm.
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Related In: Results  -  Collection

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fig04: Local matrigel injection increases the myocardial homing of c-Kit+ cells. Representative immunostaining for c-Kit in the three groups of animals. c-Kit+ cells (green) were identified in infarct border zone at 4 weeks after treatment. Red, TOPRO3 in nuclei. Yellow is shown after green [(A), sham, (B), MI-PBS, (C–F), MI-M]. (D) The number of c-Kit+ cells per HPF in MI-M hearts (n= 5) was significantly higher than in MI hearts (n= 5), and c-Kit+ cells can seldom be seen in sham animals (n= 5). *P < 0.01. (A–C) scale bars = 250 μm; (D–F) scale bars = 25 μm.
Mentions: To examine whether local matrigel delivery can home c-Kit+ and CD34+ cells to infracted myocardial zone, c-Kit+ and CD34+ cell number in the infarct zone of heart were assessed by immunostaining at 4 weeks after MI (Figs 4 and 5). There was a significant increase in the number of c-Kit+ cells in the MI-M group compared to the MI-PBS group (38.3 ± 5.3 versus 25.7 ± 1.5 c-Kit+ cells per HPF [630×], n= 5, P < 0.05, Fig. 4D). Similarly, the number of CD34+ was also significantly increased compared with MI-PBS (13.0 ± 1.51 versus 5.6 ± 0.67 CD34+ cells per HPF [630×], n= 5, P < 0.01, Fig. 5D). The c-Kit+ cells can seldom be seen in sham-operated rat. These observations therefore revealed that matrigel may enhance stem cells recruitment after MI.

Bottom Line: Compared to the MI control group (MI-PBS), matrigel significantly improved left ventricular function (n= 11, P < 0.05) assessed by pressure-volume loops after 4 weeks.The infarct wall thickness of left ventricle is significantly higher (P < 0.01) in MI-M (0.72 ± 0.02 mm, n = 10) compared with MI-PBS (0.62 ± 0.02 mm, n = 10).MI-M hearts exhibited higher capillary density (border 130.8 ± 4.7 versus 115.4 ± 6.0, P < 0.05; vessels per high-power field [HPF; 400×], n = 6) than MI-PBS hearts. c-Kit(+) stem cells (38.3 ± 5.3 versus 25.7 ± 1.5 c-Kit(+) cells per HPF [630×], n = 5, P < 0.05) and CD34(+) cells (13.0 ± 1.51 versus 5.6 ± 0.68 CD34(+) cells per HPF [630×], n = 5, P < 0.01) were significantly more numerous in MI-M than in MI-PBS in the infarcted hearts (n = 5, P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Reference- and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany.

Show MeSH
Related in: MedlinePlus