Intracardiac injection of matrigel induces stem cell recruitment and improves cardiac functions in a rat myocardial infarction model.
Bottom Line: Compared to the MI control group (MI-PBS), matrigel significantly improved left ventricular function (n= 11, P < 0.05) assessed by pressure-volume loops after 4 weeks.MI-M hearts exhibited higher capillary density (border 130.8 ± 4.7 versus 115.4 ± 6.0, P < 0.05; vessels per high-power field [HPF; 400×], n = 6) than MI-PBS hearts. c-Kit(+) stem cells (38.3 ± 5.3 versus 25.7 ± 1.5 c-Kit(+) cells per HPF [630×], n = 5, P < 0.05) and CD34(+) cells (13.0 ± 1.51 versus 5.6 ± 0.68 CD34(+) cells per HPF [630×], n = 5, P < 0.01) were significantly more numerous in MI-M than in MI-PBS in the infarcted hearts (n = 5, P < 0.05).Intracardiac matrigel injection restores myocardial functions following MI, which may attribute to the improved recruitment of CD34(+) and c-Kit(+) stem cells.
Affiliation: Reference- and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany.Show MeSH
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Mentions: LAD ligation consistently resulted in transmural MI, exhibiting typical histological changes including thinning of the left ventricular free wall and extensive collagen deposition 4 weeks after MI. The effect of matrigel treatment on myocardial injury 4 weeks after infarction was evaluated by Sirius red/Fast Green staining (Fig. 2A and B). Mean infarct size in the PBS control animals was 21.48 ± 1.49% of the whole heart (n= 10, Fig. 2C). Compared with the PBS group, injection of matrigel has no significant reduction the infarct size (20.98 ± 1.25%, n= 10, Fig. 2C). However the infarct LWT is significantly higher (P < 0.01) in MI-M (0.72 ± 0.02 mm, n= 10) compared with MI-PBS (0.62 ± 0.02 mm, n= 10, Fig. 2D).
Affiliation: Reference- and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany.