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Intracardiac injection of matrigel induces stem cell recruitment and improves cardiac functions in a rat myocardial infarction model.

Ou L, Li W, Zhang Y, Wang W, Liu J, Sorg H, Furlani D, Gäbel R, Mark P, Klopsch C, Wang L, Lützow K, Lendlein A, Wagner K, Klee D, Liebold A, Li RK, Kong D, Steinhoff G, Ma N - J. Cell. Mol. Med. (2010)

Bottom Line: Compared to the MI control group (MI-PBS), matrigel significantly improved left ventricular function (n= 11, P < 0.05) assessed by pressure-volume loops after 4 weeks.The infarct wall thickness of left ventricle is significantly higher (P < 0.01) in MI-M (0.72 ± 0.02 mm, n = 10) compared with MI-PBS (0.62 ± 0.02 mm, n = 10).MI-M hearts exhibited higher capillary density (border 130.8 ± 4.7 versus 115.4 ± 6.0, P < 0.05; vessels per high-power field [HPF; 400×], n = 6) than MI-PBS hearts. c-Kit(+) stem cells (38.3 ± 5.3 versus 25.7 ± 1.5 c-Kit(+) cells per HPF [630×], n = 5, P < 0.05) and CD34(+) cells (13.0 ± 1.51 versus 5.6 ± 0.68 CD34(+) cells per HPF [630×], n = 5, P < 0.01) were significantly more numerous in MI-M than in MI-PBS in the infarcted hearts (n = 5, P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Reference- and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany.

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Effects of local matrigel injection on cardiac remodelling 4 weeks after MI. (A), (B) Representative heart cross sections stained with Sirius Red (red, fibrosis) and Fast Green FCF (green, myocyte) from rats. (C) Ratio of infarction size to entire heart is not significantly decreased in MI-M (n= 10) compared with MI-PBS (n= 10). (D) The infarct LWT is significantly higher in MI-M (n= 10) compared with MI-PBS (n= 10). *P < 0.01.
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fig02: Effects of local matrigel injection on cardiac remodelling 4 weeks after MI. (A), (B) Representative heart cross sections stained with Sirius Red (red, fibrosis) and Fast Green FCF (green, myocyte) from rats. (C) Ratio of infarction size to entire heart is not significantly decreased in MI-M (n= 10) compared with MI-PBS (n= 10). (D) The infarct LWT is significantly higher in MI-M (n= 10) compared with MI-PBS (n= 10). *P < 0.01.

Mentions: LAD ligation consistently resulted in transmural MI, exhibiting typical histological changes including thinning of the left ventricular free wall and extensive collagen deposition 4 weeks after MI. The effect of matrigel treatment on myocardial injury 4 weeks after infarction was evaluated by Sirius red/Fast Green staining (Fig. 2A and B). Mean infarct size in the PBS control animals was 21.48 ± 1.49% of the whole heart (n= 10, Fig. 2C). Compared with the PBS group, injection of matrigel has no significant reduction the infarct size (20.98 ± 1.25%, n= 10, Fig. 2C). However the infarct LWT is significantly higher (P < 0.01) in MI-M (0.72 ± 0.02 mm, n= 10) compared with MI-PBS (0.62 ± 0.02 mm, n= 10, Fig. 2D).


Intracardiac injection of matrigel induces stem cell recruitment and improves cardiac functions in a rat myocardial infarction model.

Ou L, Li W, Zhang Y, Wang W, Liu J, Sorg H, Furlani D, Gäbel R, Mark P, Klopsch C, Wang L, Lützow K, Lendlein A, Wagner K, Klee D, Liebold A, Li RK, Kong D, Steinhoff G, Ma N - J. Cell. Mol. Med. (2010)

Effects of local matrigel injection on cardiac remodelling 4 weeks after MI. (A), (B) Representative heart cross sections stained with Sirius Red (red, fibrosis) and Fast Green FCF (green, myocyte) from rats. (C) Ratio of infarction size to entire heart is not significantly decreased in MI-M (n= 10) compared with MI-PBS (n= 10). (D) The infarct LWT is significantly higher in MI-M (n= 10) compared with MI-PBS (n= 10). *P < 0.01.
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373331&req=5

fig02: Effects of local matrigel injection on cardiac remodelling 4 weeks after MI. (A), (B) Representative heart cross sections stained with Sirius Red (red, fibrosis) and Fast Green FCF (green, myocyte) from rats. (C) Ratio of infarction size to entire heart is not significantly decreased in MI-M (n= 10) compared with MI-PBS (n= 10). (D) The infarct LWT is significantly higher in MI-M (n= 10) compared with MI-PBS (n= 10). *P < 0.01.
Mentions: LAD ligation consistently resulted in transmural MI, exhibiting typical histological changes including thinning of the left ventricular free wall and extensive collagen deposition 4 weeks after MI. The effect of matrigel treatment on myocardial injury 4 weeks after infarction was evaluated by Sirius red/Fast Green staining (Fig. 2A and B). Mean infarct size in the PBS control animals was 21.48 ± 1.49% of the whole heart (n= 10, Fig. 2C). Compared with the PBS group, injection of matrigel has no significant reduction the infarct size (20.98 ± 1.25%, n= 10, Fig. 2C). However the infarct LWT is significantly higher (P < 0.01) in MI-M (0.72 ± 0.02 mm, n= 10) compared with MI-PBS (0.62 ± 0.02 mm, n= 10, Fig. 2D).

Bottom Line: Compared to the MI control group (MI-PBS), matrigel significantly improved left ventricular function (n= 11, P < 0.05) assessed by pressure-volume loops after 4 weeks.The infarct wall thickness of left ventricle is significantly higher (P < 0.01) in MI-M (0.72 ± 0.02 mm, n = 10) compared with MI-PBS (0.62 ± 0.02 mm, n = 10).MI-M hearts exhibited higher capillary density (border 130.8 ± 4.7 versus 115.4 ± 6.0, P < 0.05; vessels per high-power field [HPF; 400×], n = 6) than MI-PBS hearts. c-Kit(+) stem cells (38.3 ± 5.3 versus 25.7 ± 1.5 c-Kit(+) cells per HPF [630×], n = 5, P < 0.05) and CD34(+) cells (13.0 ± 1.51 versus 5.6 ± 0.68 CD34(+) cells per HPF [630×], n = 5, P < 0.01) were significantly more numerous in MI-M than in MI-PBS in the infarcted hearts (n = 5, P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Reference- and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany.

Show MeSH
Related in: MedlinePlus