Intracardiac injection of matrigel induces stem cell recruitment and improves cardiac functions in a rat myocardial infarction model.
Bottom Line: Compared to the MI control group (MI-PBS), matrigel significantly improved left ventricular function (n= 11, P < 0.05) assessed by pressure-volume loops after 4 weeks.The infarct wall thickness of left ventricle is significantly higher (P < 0.01) in MI-M (0.72 ± 0.02 mm, n = 10) compared with MI-PBS (0.62 ± 0.02 mm, n = 10).MI-M hearts exhibited higher capillary density (border 130.8 ± 4.7 versus 115.4 ± 6.0, P < 0.05; vessels per high-power field [HPF; 400×], n = 6) than MI-PBS hearts. c-Kit(+) stem cells (38.3 ± 5.3 versus 25.7 ± 1.5 c-Kit(+) cells per HPF [630×], n = 5, P < 0.05) and CD34(+) cells (13.0 ± 1.51 versus 5.6 ± 0.68 CD34(+) cells per HPF [630×], n = 5, P < 0.01) were significantly more numerous in MI-M than in MI-PBS in the infarcted hearts (n = 5, P < 0.05).
Affiliation: Reference- and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany.Show MeSH
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Mentions: We then evaluated the ventricular function by pressure–volume loop method. Matrigel treatment enhanced systolic and diastolic properties of the infracted left ventricle. Haemodynamic changes are summarized in Table 1. Matrigel treatment produced a 22.7% increase in left ventricular ejection fraction (left ventricular-EF, P= 0.008, Fig. 1) relative to MI-PBS (Table 1). Left ventricular peak rate of pressure rise (left ventricular dp/dt max, Fig. 1), a commonly used index of myocardial contractility was significantly enhanced (P= 0.006) when compared with MI-PBS. Moreover, we also observed a 24.5% increased in the peak rate of left ventricular pressure decline (left ventricular dp/dt min) compared with MI-PBS (Fig. 1), demonstrating enhanced relaxation in MI-M. Taken together, these results demonstrate that direct administration of matrigel enhanced left ventricular-EF recovery and improved contraction kinetics of left ventricle.
Affiliation: Reference- and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany.