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The role of CXCR7 on the adhesion, proliferation and angiogenesis of endothelial progenitor cells.

Dai X, Tan Y, Cai S, Xiong X, Wang L, Ye Q, Yan X, Ma K, Cai L - J. Cell. Mol. Med. (2011)

Bottom Line: CXC chemokine receptor 4 (CXCR4) has been considered as the unique receptor of SDF-1 and as the only mediator of SDF-1-induced biological effects for many years.However, recent studies found that SDF-1 could bind to not only CXCR4 but also CXC chemokine receptor 7 (CXCR7).These results suggested that both CXCR7 and CXCR4 are important for EPCs in response to SDF-1, indicating that CXCR7 may be another potential target molecule for angiogenesis-dependent diseases.

View Article: PubMed Central - PubMed

Affiliation: Key laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, China.

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The effects of CXCR7 and CXCR4 on SDF-1-induced angiogenesis in vitro. Angiogenesis was assayed by measuring the lengths of tube-like structures under a light microscope equipped with a digital camera in a blind manner. (A) EPCs were cultured on the matrigel with different concentrations of SDF-1 or without SDF-1 for 72 hrs. Representative images of tube-like structures from different groups (left panel) and quantitative data (right panel) are presented. (B) EPCs cultured on the matrigel with SDF-1 (10 ng/ml) were simultaneously treated with and without anti-CXCR4 antibody (α CXCR4), anti-CXCR7 antibody (α CXCR7), IgG control, AMD3100 or CCX733, respectively, for 72 hrs. The lengths of tube-like structures were measured as (A). Data are given as mean ± S.D. (**P < 0.01, versus control without SDF-1; #P < 0.05, ##P < 0.01, versus SDF-1). Scale bars represent 100 μM.
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fig08: The effects of CXCR7 and CXCR4 on SDF-1-induced angiogenesis in vitro. Angiogenesis was assayed by measuring the lengths of tube-like structures under a light microscope equipped with a digital camera in a blind manner. (A) EPCs were cultured on the matrigel with different concentrations of SDF-1 or without SDF-1 for 72 hrs. Representative images of tube-like structures from different groups (left panel) and quantitative data (right panel) are presented. (B) EPCs cultured on the matrigel with SDF-1 (10 ng/ml) were simultaneously treated with and without anti-CXCR4 antibody (α CXCR4), anti-CXCR7 antibody (α CXCR7), IgG control, AMD3100 or CCX733, respectively, for 72 hrs. The lengths of tube-like structures were measured as (A). Data are given as mean ± S.D. (**P < 0.01, versus control without SDF-1; #P < 0.05, ##P < 0.01, versus SDF-1). Scale bars represent 100 μM.

Mentions: EPCs in EBM-2 medium containing 1 ng/ml, 10 ng/ml or 100 ng/ml SDF-1 was planted on matrigel, and length of tube-like structures was measured. SDF-1 (10–100 ng/ml) significantly induced EPC tube formation (Fig. 8A). To evaluate the roles of CXCR4 and CXCR7 in SDF-1-induced angiogenesis, we treated SDF-1-stimulated EPCs with CXCR4 and CXCR7 antibodies or antagonists, respectively, and then measured tube length. As shown in Figure 8B, CXCR4 antibody or antagonist AMD3100 significantly decreased the length of SDF-1-induced tubes in comparison of the group without blocking CXCR4 (1.31 ± 0.19 or 1.59 ± 0.26 versus 2.4 ± 0.48, P < 0.01); Similarly CXCR7 antibody or antagonist CCX733 also significantly inhibited SDF-1-induced EPC tube formation (1.08 ± 0.29 or 1.40 ± 0.133 versus 2.4 ± 0.48, P < 0.01). These results suggest that SDF-1-induced tube formation from EPCs through both CXCR4 and CXCR7. When blocking both of CXCR4 and CXCR7 with their antibodies or antagonists, the effect of SDF-1 on tube formation was completely abolished.


The role of CXCR7 on the adhesion, proliferation and angiogenesis of endothelial progenitor cells.

Dai X, Tan Y, Cai S, Xiong X, Wang L, Ye Q, Yan X, Ma K, Cai L - J. Cell. Mol. Med. (2011)

The effects of CXCR7 and CXCR4 on SDF-1-induced angiogenesis in vitro. Angiogenesis was assayed by measuring the lengths of tube-like structures under a light microscope equipped with a digital camera in a blind manner. (A) EPCs were cultured on the matrigel with different concentrations of SDF-1 or without SDF-1 for 72 hrs. Representative images of tube-like structures from different groups (left panel) and quantitative data (right panel) are presented. (B) EPCs cultured on the matrigel with SDF-1 (10 ng/ml) were simultaneously treated with and without anti-CXCR4 antibody (α CXCR4), anti-CXCR7 antibody (α CXCR7), IgG control, AMD3100 or CCX733, respectively, for 72 hrs. The lengths of tube-like structures were measured as (A). Data are given as mean ± S.D. (**P < 0.01, versus control without SDF-1; #P < 0.05, ##P < 0.01, versus SDF-1). Scale bars represent 100 μM.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4373330&req=5

fig08: The effects of CXCR7 and CXCR4 on SDF-1-induced angiogenesis in vitro. Angiogenesis was assayed by measuring the lengths of tube-like structures under a light microscope equipped with a digital camera in a blind manner. (A) EPCs were cultured on the matrigel with different concentrations of SDF-1 or without SDF-1 for 72 hrs. Representative images of tube-like structures from different groups (left panel) and quantitative data (right panel) are presented. (B) EPCs cultured on the matrigel with SDF-1 (10 ng/ml) were simultaneously treated with and without anti-CXCR4 antibody (α CXCR4), anti-CXCR7 antibody (α CXCR7), IgG control, AMD3100 or CCX733, respectively, for 72 hrs. The lengths of tube-like structures were measured as (A). Data are given as mean ± S.D. (**P < 0.01, versus control without SDF-1; #P < 0.05, ##P < 0.01, versus SDF-1). Scale bars represent 100 μM.
Mentions: EPCs in EBM-2 medium containing 1 ng/ml, 10 ng/ml or 100 ng/ml SDF-1 was planted on matrigel, and length of tube-like structures was measured. SDF-1 (10–100 ng/ml) significantly induced EPC tube formation (Fig. 8A). To evaluate the roles of CXCR4 and CXCR7 in SDF-1-induced angiogenesis, we treated SDF-1-stimulated EPCs with CXCR4 and CXCR7 antibodies or antagonists, respectively, and then measured tube length. As shown in Figure 8B, CXCR4 antibody or antagonist AMD3100 significantly decreased the length of SDF-1-induced tubes in comparison of the group without blocking CXCR4 (1.31 ± 0.19 or 1.59 ± 0.26 versus 2.4 ± 0.48, P < 0.01); Similarly CXCR7 antibody or antagonist CCX733 also significantly inhibited SDF-1-induced EPC tube formation (1.08 ± 0.29 or 1.40 ± 0.133 versus 2.4 ± 0.48, P < 0.01). These results suggest that SDF-1-induced tube formation from EPCs through both CXCR4 and CXCR7. When blocking both of CXCR4 and CXCR7 with their antibodies or antagonists, the effect of SDF-1 on tube formation was completely abolished.

Bottom Line: CXC chemokine receptor 4 (CXCR4) has been considered as the unique receptor of SDF-1 and as the only mediator of SDF-1-induced biological effects for many years.However, recent studies found that SDF-1 could bind to not only CXCR4 but also CXC chemokine receptor 7 (CXCR7).These results suggested that both CXCR7 and CXCR4 are important for EPCs in response to SDF-1, indicating that CXCR7 may be another potential target molecule for angiogenesis-dependent diseases.

View Article: PubMed Central - PubMed

Affiliation: Key laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, China.

Show MeSH
Related in: MedlinePlus