The role of CXCR7 on the adhesion, proliferation and angiogenesis of endothelial progenitor cells.
Bottom Line: CXC chemokine receptor 4 (CXCR4) has been considered as the unique receptor of SDF-1 and as the only mediator of SDF-1-induced biological effects for many years.However, recent studies found that SDF-1 could bind to not only CXCR4 but also CXC chemokine receptor 7 (CXCR7).These results suggested that both CXCR7 and CXCR4 are important for EPCs in response to SDF-1, indicating that CXCR7 may be another potential target molecule for angiogenesis-dependent diseases.
Affiliation: Key laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, China.Show MeSH
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Mentions: EPCs in EBM-2 medium containing 1 ng/ml, 10 ng/ml or 100 ng/ml SDF-1 was planted on matrigel, and length of tube-like structures was measured. SDF-1 (10–100 ng/ml) significantly induced EPC tube formation (Fig. 8A). To evaluate the roles of CXCR4 and CXCR7 in SDF-1-induced angiogenesis, we treated SDF-1-stimulated EPCs with CXCR4 and CXCR7 antibodies or antagonists, respectively, and then measured tube length. As shown in Figure 8B, CXCR4 antibody or antagonist AMD3100 significantly decreased the length of SDF-1-induced tubes in comparison of the group without blocking CXCR4 (1.31 ± 0.19 or 1.59 ± 0.26 versus 2.4 ± 0.48, P < 0.01); Similarly CXCR7 antibody or antagonist CCX733 also significantly inhibited SDF-1-induced EPC tube formation (1.08 ± 0.29 or 1.40 ± 0.133 versus 2.4 ± 0.48, P < 0.01). These results suggest that SDF-1-induced tube formation from EPCs through both CXCR4 and CXCR7. When blocking both of CXCR4 and CXCR7 with their antibodies or antagonists, the effect of SDF-1 on tube formation was completely abolished.
Affiliation: Key laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, China.