Limits...
The role of CXCR7 on the adhesion, proliferation and angiogenesis of endothelial progenitor cells.

Dai X, Tan Y, Cai S, Xiong X, Wang L, Ye Q, Yan X, Ma K, Cai L - J. Cell. Mol. Med. (2011)

Bottom Line: CXC chemokine receptor 4 (CXCR4) has been considered as the unique receptor of SDF-1 and as the only mediator of SDF-1-induced biological effects for many years.However, recent studies found that SDF-1 could bind to not only CXCR4 but also CXC chemokine receptor 7 (CXCR7).These results suggested that both CXCR7 and CXCR4 are important for EPCs in response to SDF-1, indicating that CXCR7 may be another potential target molecule for angiogenesis-dependent diseases.

View Article: PubMed Central - PubMed

Affiliation: Key laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, China.

Show MeSH

Related in: MedlinePlus

SDF-1-promoted EPC survival predominantly via CXCR7. EPCs were cultured for 48 hrs in serum-free medium supplemented with SDF-1 (10 ng/ml) in the presence or absence of anti-CXCR4 antibody (α CXCR4), anti-CXCR7 antibody (α CXCR7), IgG control, AMD3100 or CCX733, respectively, and then the apoptotic cells were assessed by staining with FITC-conjugated annexin V and PI and evaluated by flow cytometry. (A) Representative flow cytometry gating data for annexin+ cells in EPCs with different treatments. (B) The apoptotic cells percentage was summarized and presented as mean ± S.D. (**P < 0.01, versus control without SDF-1; ##P < 0.01, versus SDF-1).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4373330&req=5

fig07: SDF-1-promoted EPC survival predominantly via CXCR7. EPCs were cultured for 48 hrs in serum-free medium supplemented with SDF-1 (10 ng/ml) in the presence or absence of anti-CXCR4 antibody (α CXCR4), anti-CXCR7 antibody (α CXCR7), IgG control, AMD3100 or CCX733, respectively, and then the apoptotic cells were assessed by staining with FITC-conjugated annexin V and PI and evaluated by flow cytometry. (A) Representative flow cytometry gating data for annexin+ cells in EPCs with different treatments. (B) The apoptotic cells percentage was summarized and presented as mean ± S.D. (**P < 0.01, versus control without SDF-1; ##P < 0.01, versus SDF-1).

Mentions: To examine the role of CXCR4 and CXCR7 in EPC survival, we determined serum deprivation-induced EPCs apoptosis by flow cytometry with FITC-conjugated annexin V and PI staining. As shown in Figure 7, serum deprivation induced about 19% of apoptotic cell death, but this apoptotic cell death can be significantly prevented with treatment of SDF-1 for 48 hrs (7.47 ± 0.71%versus 18.93 ± 2.58%, P < 0.01). The anti-apoptotic effect of SDF-1 was almost completely attenuated by CXCR7 antibody or antagonist CCX733 (16.05 ± 1.69% or 18.02 ± 1.95%versus 7.47 ± 0.71%, P < 0.01). However, blocking CXCR4 with anti-CXCR4 antibody or CXCR4 antagonist AMD3100 could not significantly diminish the anti-apoptotic effect of SDF-1 on the EPCs with serum deprivation. In addition, blocking both CXCR4 and CXCR7 has a similar effect to blocking CXCR7 alone in the protection of EPCs from apoptosis. Collectively, the results suggest that SDF-1 mediates EPC survival predominantly via CXCR7.


The role of CXCR7 on the adhesion, proliferation and angiogenesis of endothelial progenitor cells.

Dai X, Tan Y, Cai S, Xiong X, Wang L, Ye Q, Yan X, Ma K, Cai L - J. Cell. Mol. Med. (2011)

SDF-1-promoted EPC survival predominantly via CXCR7. EPCs were cultured for 48 hrs in serum-free medium supplemented with SDF-1 (10 ng/ml) in the presence or absence of anti-CXCR4 antibody (α CXCR4), anti-CXCR7 antibody (α CXCR7), IgG control, AMD3100 or CCX733, respectively, and then the apoptotic cells were assessed by staining with FITC-conjugated annexin V and PI and evaluated by flow cytometry. (A) Representative flow cytometry gating data for annexin+ cells in EPCs with different treatments. (B) The apoptotic cells percentage was summarized and presented as mean ± S.D. (**P < 0.01, versus control without SDF-1; ##P < 0.01, versus SDF-1).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373330&req=5

fig07: SDF-1-promoted EPC survival predominantly via CXCR7. EPCs were cultured for 48 hrs in serum-free medium supplemented with SDF-1 (10 ng/ml) in the presence or absence of anti-CXCR4 antibody (α CXCR4), anti-CXCR7 antibody (α CXCR7), IgG control, AMD3100 or CCX733, respectively, and then the apoptotic cells were assessed by staining with FITC-conjugated annexin V and PI and evaluated by flow cytometry. (A) Representative flow cytometry gating data for annexin+ cells in EPCs with different treatments. (B) The apoptotic cells percentage was summarized and presented as mean ± S.D. (**P < 0.01, versus control without SDF-1; ##P < 0.01, versus SDF-1).
Mentions: To examine the role of CXCR4 and CXCR7 in EPC survival, we determined serum deprivation-induced EPCs apoptosis by flow cytometry with FITC-conjugated annexin V and PI staining. As shown in Figure 7, serum deprivation induced about 19% of apoptotic cell death, but this apoptotic cell death can be significantly prevented with treatment of SDF-1 for 48 hrs (7.47 ± 0.71%versus 18.93 ± 2.58%, P < 0.01). The anti-apoptotic effect of SDF-1 was almost completely attenuated by CXCR7 antibody or antagonist CCX733 (16.05 ± 1.69% or 18.02 ± 1.95%versus 7.47 ± 0.71%, P < 0.01). However, blocking CXCR4 with anti-CXCR4 antibody or CXCR4 antagonist AMD3100 could not significantly diminish the anti-apoptotic effect of SDF-1 on the EPCs with serum deprivation. In addition, blocking both CXCR4 and CXCR7 has a similar effect to blocking CXCR7 alone in the protection of EPCs from apoptosis. Collectively, the results suggest that SDF-1 mediates EPC survival predominantly via CXCR7.

Bottom Line: CXC chemokine receptor 4 (CXCR4) has been considered as the unique receptor of SDF-1 and as the only mediator of SDF-1-induced biological effects for many years.However, recent studies found that SDF-1 could bind to not only CXCR4 but also CXC chemokine receptor 7 (CXCR7).These results suggested that both CXCR7 and CXCR4 are important for EPCs in response to SDF-1, indicating that CXCR7 may be another potential target molecule for angiogenesis-dependent diseases.

View Article: PubMed Central - PubMed

Affiliation: Key laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, China.

Show MeSH
Related in: MedlinePlus