The role of CXCR7 on the adhesion, proliferation and angiogenesis of endothelial progenitor cells.
Bottom Line: CXC chemokine receptor 4 (CXCR4) has been considered as the unique receptor of SDF-1 and as the only mediator of SDF-1-induced biological effects for many years.However, recent studies found that SDF-1 could bind to not only CXCR4 but also CXC chemokine receptor 7 (CXCR7).These results suggested that both CXCR7 and CXCR4 are important for EPCs in response to SDF-1, indicating that CXCR7 may be another potential target molecule for angiogenesis-dependent diseases.
Affiliation: Key laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, China.Show MeSH
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Mentions: The adhesion capacity of EPCs to the activated endothelial cells and the extracellular matrix are very important for EPC participating in angiogenesis . We tested whether SDF-1 promotes EPC adhesion to extracellular matrix or endothelial cells using an in vitro cell adhesion assay. As shown in Figure 3A and B, Both CXCR7 antibody and CXCR4 antibody significantly inhibit SDF-1-mediated EPC adhesion to collagen (Fig. 3A) and fibronectin (Fig. 3B). The inhibitory effect of either CXCR7 antibody or CXCR4 antibody was confirmed with either CXCR7 antagonist CCX733 or CXCR4 antagonist AMD3100 (Fig. 3A and B). Although both CXCR4 and CXCR7 antibodies or antagonists could inhibit the adhesion of EPCs to fibronectin, the inhibitory effect of CXCR7 antibody or antagonist is more predominant. There was no significant additive effect when both CXCR4 and CXCR7 were blocked either with their antibodies or antagonists.
Affiliation: Key laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, China.