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The role of CXCR7 on the adhesion, proliferation and angiogenesis of endothelial progenitor cells.

Dai X, Tan Y, Cai S, Xiong X, Wang L, Ye Q, Yan X, Ma K, Cai L - J. Cell. Mol. Med. (2011)

Bottom Line: CXC chemokine receptor 4 (CXCR4) has been considered as the unique receptor of SDF-1 and as the only mediator of SDF-1-induced biological effects for many years.However, recent studies found that SDF-1 could bind to not only CXCR4 but also CXC chemokine receptor 7 (CXCR7).These results suggested that both CXCR7 and CXCR4 are important for EPCs in response to SDF-1, indicating that CXCR7 may be another potential target molecule for angiogenesis-dependent diseases.

View Article: PubMed Central - PubMed

Affiliation: Key laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, China.

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The adhesion properties of EPCs to ECM components and HUVEC induced by SDF-1. EPCs pre-treated with anti-CXCR4 antibody (α CXCR4), anti-CXCR7 antibody (α CXCR7), IgG control, AMD3100 or CCX733, respectively, were added onto ECM components [collagen (A) and fibronectin (B)] or HUVEC monolayer bond with SDF-1 (C) for 30 min., and then the number of adherent cells was counted in multiple microscopic 10× fields. Data are given as mean ± S.D. (*P < 0.05; **P < 0.01 versus control).
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fig03: The adhesion properties of EPCs to ECM components and HUVEC induced by SDF-1. EPCs pre-treated with anti-CXCR4 antibody (α CXCR4), anti-CXCR7 antibody (α CXCR7), IgG control, AMD3100 or CCX733, respectively, were added onto ECM components [collagen (A) and fibronectin (B)] or HUVEC monolayer bond with SDF-1 (C) for 30 min., and then the number of adherent cells was counted in multiple microscopic 10× fields. Data are given as mean ± S.D. (*P < 0.05; **P < 0.01 versus control).

Mentions: The adhesion capacity of EPCs to the activated endothelial cells and the extracellular matrix are very important for EPC participating in angiogenesis [28]. We tested whether SDF-1 promotes EPC adhesion to extracellular matrix or endothelial cells using an in vitro cell adhesion assay. As shown in Figure 3A and B, Both CXCR7 antibody and CXCR4 antibody significantly inhibit SDF-1-mediated EPC adhesion to collagen (Fig. 3A) and fibronectin (Fig. 3B). The inhibitory effect of either CXCR7 antibody or CXCR4 antibody was confirmed with either CXCR7 antagonist CCX733 or CXCR4 antagonist AMD3100 (Fig. 3A and B). Although both CXCR4 and CXCR7 antibodies or antagonists could inhibit the adhesion of EPCs to fibronectin, the inhibitory effect of CXCR7 antibody or antagonist is more predominant. There was no significant additive effect when both CXCR4 and CXCR7 were blocked either with their antibodies or antagonists.


The role of CXCR7 on the adhesion, proliferation and angiogenesis of endothelial progenitor cells.

Dai X, Tan Y, Cai S, Xiong X, Wang L, Ye Q, Yan X, Ma K, Cai L - J. Cell. Mol. Med. (2011)

The adhesion properties of EPCs to ECM components and HUVEC induced by SDF-1. EPCs pre-treated with anti-CXCR4 antibody (α CXCR4), anti-CXCR7 antibody (α CXCR7), IgG control, AMD3100 or CCX733, respectively, were added onto ECM components [collagen (A) and fibronectin (B)] or HUVEC monolayer bond with SDF-1 (C) for 30 min., and then the number of adherent cells was counted in multiple microscopic 10× fields. Data are given as mean ± S.D. (*P < 0.05; **P < 0.01 versus control).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373330&req=5

fig03: The adhesion properties of EPCs to ECM components and HUVEC induced by SDF-1. EPCs pre-treated with anti-CXCR4 antibody (α CXCR4), anti-CXCR7 antibody (α CXCR7), IgG control, AMD3100 or CCX733, respectively, were added onto ECM components [collagen (A) and fibronectin (B)] or HUVEC monolayer bond with SDF-1 (C) for 30 min., and then the number of adherent cells was counted in multiple microscopic 10× fields. Data are given as mean ± S.D. (*P < 0.05; **P < 0.01 versus control).
Mentions: The adhesion capacity of EPCs to the activated endothelial cells and the extracellular matrix are very important for EPC participating in angiogenesis [28]. We tested whether SDF-1 promotes EPC adhesion to extracellular matrix or endothelial cells using an in vitro cell adhesion assay. As shown in Figure 3A and B, Both CXCR7 antibody and CXCR4 antibody significantly inhibit SDF-1-mediated EPC adhesion to collagen (Fig. 3A) and fibronectin (Fig. 3B). The inhibitory effect of either CXCR7 antibody or CXCR4 antibody was confirmed with either CXCR7 antagonist CCX733 or CXCR4 antagonist AMD3100 (Fig. 3A and B). Although both CXCR4 and CXCR7 antibodies or antagonists could inhibit the adhesion of EPCs to fibronectin, the inhibitory effect of CXCR7 antibody or antagonist is more predominant. There was no significant additive effect when both CXCR4 and CXCR7 were blocked either with their antibodies or antagonists.

Bottom Line: CXC chemokine receptor 4 (CXCR4) has been considered as the unique receptor of SDF-1 and as the only mediator of SDF-1-induced biological effects for many years.However, recent studies found that SDF-1 could bind to not only CXCR4 but also CXC chemokine receptor 7 (CXCR7).These results suggested that both CXCR7 and CXCR4 are important for EPCs in response to SDF-1, indicating that CXCR7 may be another potential target molecule for angiogenesis-dependent diseases.

View Article: PubMed Central - PubMed

Affiliation: Key laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, China.

Show MeSH
Related in: MedlinePlus