Limits...
The role of CXCR7 on the adhesion, proliferation and angiogenesis of endothelial progenitor cells.

Dai X, Tan Y, Cai S, Xiong X, Wang L, Ye Q, Yan X, Ma K, Cai L - J. Cell. Mol. Med. (2011)

Bottom Line: CXC chemokine receptor 4 (CXCR4) has been considered as the unique receptor of SDF-1 and as the only mediator of SDF-1-induced biological effects for many years.However, recent studies found that SDF-1 could bind to not only CXCR4 but also CXC chemokine receptor 7 (CXCR7).These results suggested that both CXCR7 and CXCR4 are important for EPCs in response to SDF-1, indicating that CXCR7 may be another potential target molecule for angiogenesis-dependent diseases.

View Article: PubMed Central - PubMed

Affiliation: Key laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, China.

Show MeSH

Related in: MedlinePlus

The expression of CXCR7 and CXCR4 on EPCs. The expression of CXCR7 and CXCR4 on EPCs was detected by RT-PCR analysis for the mRNA (A) [M: Maker, DL2000; 1: GAPDH (618 bp); 2: CXCR7 (473 bp); 3: CXCR4 (446 bp)] and by Western blot analysis for the protein (B), respectively. GAPDH was used as loading control and HUVECs were used as CXCR7 and CXCR4 expression positive control. The cell surface expression of CXCR7 and CXCR4 was investigated by flow cytometry (C).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4373330&req=5

fig02: The expression of CXCR7 and CXCR4 on EPCs. The expression of CXCR7 and CXCR4 on EPCs was detected by RT-PCR analysis for the mRNA (A) [M: Maker, DL2000; 1: GAPDH (618 bp); 2: CXCR7 (473 bp); 3: CXCR4 (446 bp)] and by Western blot analysis for the protein (B), respectively. GAPDH was used as loading control and HUVECs were used as CXCR7 and CXCR4 expression positive control. The cell surface expression of CXCR7 and CXCR4 was investigated by flow cytometry (C).

Mentions: RT-PCR analysis revealed that the receptors of SDF-1, CXCR4 and CXCR7 were expressed highly in EPCs (Fig. 2A), which was also confirmed at the protein level by Western blot analysis (Fig. 2B) and flow cytometric analysis (Fig. 2C).


The role of CXCR7 on the adhesion, proliferation and angiogenesis of endothelial progenitor cells.

Dai X, Tan Y, Cai S, Xiong X, Wang L, Ye Q, Yan X, Ma K, Cai L - J. Cell. Mol. Med. (2011)

The expression of CXCR7 and CXCR4 on EPCs. The expression of CXCR7 and CXCR4 on EPCs was detected by RT-PCR analysis for the mRNA (A) [M: Maker, DL2000; 1: GAPDH (618 bp); 2: CXCR7 (473 bp); 3: CXCR4 (446 bp)] and by Western blot analysis for the protein (B), respectively. GAPDH was used as loading control and HUVECs were used as CXCR7 and CXCR4 expression positive control. The cell surface expression of CXCR7 and CXCR4 was investigated by flow cytometry (C).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373330&req=5

fig02: The expression of CXCR7 and CXCR4 on EPCs. The expression of CXCR7 and CXCR4 on EPCs was detected by RT-PCR analysis for the mRNA (A) [M: Maker, DL2000; 1: GAPDH (618 bp); 2: CXCR7 (473 bp); 3: CXCR4 (446 bp)] and by Western blot analysis for the protein (B), respectively. GAPDH was used as loading control and HUVECs were used as CXCR7 and CXCR4 expression positive control. The cell surface expression of CXCR7 and CXCR4 was investigated by flow cytometry (C).
Mentions: RT-PCR analysis revealed that the receptors of SDF-1, CXCR4 and CXCR7 were expressed highly in EPCs (Fig. 2A), which was also confirmed at the protein level by Western blot analysis (Fig. 2B) and flow cytometric analysis (Fig. 2C).

Bottom Line: CXC chemokine receptor 4 (CXCR4) has been considered as the unique receptor of SDF-1 and as the only mediator of SDF-1-induced biological effects for many years.However, recent studies found that SDF-1 could bind to not only CXCR4 but also CXC chemokine receptor 7 (CXCR7).These results suggested that both CXCR7 and CXCR4 are important for EPCs in response to SDF-1, indicating that CXCR7 may be another potential target molecule for angiogenesis-dependent diseases.

View Article: PubMed Central - PubMed

Affiliation: Key laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, China.

Show MeSH
Related in: MedlinePlus