Generation of easily accessible human kidney tubules on two-dimensional surfaces in vitro.
Bottom Line: However, after triggering the process, the formation of renal tubules occurs with remarkable independence from the substrate architecture.Human proximal tubules generated on 2D surfaces typically have a length of several millimetres, and are easily accessible for manipulations and imaging, which makes them attractive for basic research and in vitro nephrotoxicology.The experimental system described also allows for in vitro studies on how primary human kidney cells regenerate renal structures after organ disruption.
Affiliation: Institute of Bioengineering and Nanotechnology, The Nanos, Singapore, Singapore.Show MeSH
Related in: MedlinePlus
Mentions: Besides substrate architecture, interactions between epithelial cells and myofibroblasts appeared to be important for tubule formation. Unresolved questions included where the myofibroblasts were derived from, whether their presence was due to contaminations of the epithelial cells and what role they played in tubule formation. Figure 9C shows that α-SMA-expressing cells were not detectable by immunostaining in the initial cultures of HPTCs. The expression levels of α-SMA, as determined by qRT-PCR, were very low in such initial cultures, and were not higher than the expression levels in HeLa cells or human embryonic kidney (HEK) 293 cells (Fig. 9A). HeLa cells are negative for α-SMA , and HeLa as well HEK293 cells are well-established epithelial cell lines free of contaminations with other cell types.
Affiliation: Institute of Bioengineering and Nanotechnology, The Nanos, Singapore, Singapore.