Generation of easily accessible human kidney tubules on two-dimensional surfaces in vitro.
Bottom Line: However, after triggering the process, the formation of renal tubules occurs with remarkable independence from the substrate architecture.Human proximal tubules generated on 2D surfaces typically have a length of several millimetres, and are easily accessible for manipulations and imaging, which makes them attractive for basic research and in vitro nephrotoxicology.The experimental system described also allows for in vitro studies on how primary human kidney cells regenerate renal structures after organ disruption.
Affiliation: Institute of Bioengineering and Nanotechnology, The Nanos, Singapore, Singapore.Show MeSH
Related in: MedlinePlus
Mentions: Figure 8 shows that HPTCs formed tubules within capillaries. These results demonstrated that tubule formation was not inhibited by arranging HPTCs into a pre-formed tubular architecture. Tubule formation within capillaries was accomplished by the same process as employed on 2D surfaces, involving monolayer formation and subsequent appearance of myofibroblast aggregates. Obviously, this process would lead to hollow fibre clogging in BAKs. The results also revealed that lateral attachment to a rigid substrate was not important for the sensing of a 3D environment. Three-dimensional edges were probably sensed indirectly through the impact of the substrate architecture on surface tension and fluid dynamics of the medium .
Affiliation: Institute of Bioengineering and Nanotechnology, The Nanos, Singapore, Singapore.