Generation of easily accessible human kidney tubules on two-dimensional surfaces in vitro.
Bottom Line: However, after triggering the process, the formation of renal tubules occurs with remarkable independence from the substrate architecture.Human proximal tubules generated on 2D surfaces typically have a length of several millimetres, and are easily accessible for manipulations and imaging, which makes them attractive for basic research and in vitro nephrotoxicology.The experimental system described also allows for in vitro studies on how primary human kidney cells regenerate renal structures after organ disruption.
Affiliation: Institute of Bioengineering and Nanotechnology, The Nanos, Singapore, Singapore.Show MeSH
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Mentions: In order to determine whether the presence of a 3D edge indeed enhanced the initiation of tubule formation, we seeded cells in parallel on 18 mm cover slips (no 3D edge), 24-well cell culture plates (with 3D edge) and diagnostic printed slides (with 3D edge). Figure 7 shows that initial retraction of the epithelium first occurred at the edges of the wells of the 24-well plates (well diameter = 15 mm) and diagnostic printed slides (well diameter = 2 mm). Subsequently, monolayer reorganization as well as cell aggregate and tubule formation were observed within the wells of these devices. By contrast, the epithelial monolayer was not reorganized on cover slips during the monitoring period of 8 days, and no retraction of the monolayer occurred at the edges of the cover slips (Fig. 7).
Affiliation: Institute of Bioengineering and Nanotechnology, The Nanos, Singapore, Singapore.