Generation of easily accessible human kidney tubules on two-dimensional surfaces in vitro.
Bottom Line: However, after triggering the process, the formation of renal tubules occurs with remarkable independence from the substrate architecture.Human proximal tubules generated on 2D surfaces typically have a length of several millimetres, and are easily accessible for manipulations and imaging, which makes them attractive for basic research and in vitro nephrotoxicology.The experimental system described also allows for in vitro studies on how primary human kidney cells regenerate renal structures after organ disruption.
Affiliation: Institute of Bioengineering and Nanotechnology, The Nanos, Singapore, Singapore.Show MeSH
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Mentions: Although renal tubule formation occurred on 2D surfaces, several observations suggested that formation of 3D tissue-like structures by HPTCs was enhanced by a 3D substrate architecture. Most striking was the finding that a closed circle formed by several tubules with attached myofibroblast aggregates could be formed within a well close to its edge, with all tubules displaying a similar distance to the edge (Fig. 6A, B). By contrast to the centre of the well, the edge of the well has a 3D architecture. Another observation suggesting a crucial role for a 3D architecture in enhancing tubule formation was that initial retraction of the epithelial monolayer started in most cases first at the edge of the well (Fig. 6C, D).
Affiliation: Institute of Bioengineering and Nanotechnology, The Nanos, Singapore, Singapore.