Generation of easily accessible human kidney tubules on two-dimensional surfaces in vitro.
Bottom Line: However, after triggering the process, the formation of renal tubules occurs with remarkable independence from the substrate architecture.Human proximal tubules generated on 2D surfaces typically have a length of several millimetres, and are easily accessible for manipulations and imaging, which makes them attractive for basic research and in vitro nephrotoxicology.The experimental system described also allows for in vitro studies on how primary human kidney cells regenerate renal structures after organ disruption.
Affiliation: Institute of Bioengineering and Nanotechnology, The Nanos, Singapore, Singapore.Show MeSH
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Mentions: In order to address tubule formation by HPTCs in 3D gels, we cultured the cells in matrigel. Tubule formation in matrigel (Fig. 5E–H) involved branching of cells at the initial stages of tubule formation and outgrowth of branched cells. Outgrowing branches then formed tubules, and budding from these tubules could occur, giving rise to branched tubular structures (Fig. 5H). It was important to note that formation of epithelial monolayers and coordinated movements of large numbers of cells were not involved in tubule formation in 3D gels. The resulting human proximal tubules obtained in 3D gels were relatively small, displayed multiple branches (Fig. 5H), could be convoluted (Fig. S2), and were never attached to myofibroblast aggregates, in contrast to the tubules obtained on 2D surfaces.
Affiliation: Institute of Bioengineering and Nanotechnology, The Nanos, Singapore, Singapore.