Generation of easily accessible human kidney tubules on two-dimensional surfaces in vitro.
Bottom Line: However, after triggering the process, the formation of renal tubules occurs with remarkable independence from the substrate architecture.Human proximal tubules generated on 2D surfaces typically have a length of several millimetres, and are easily accessible for manipulations and imaging, which makes them attractive for basic research and in vitro nephrotoxicology.The experimental system described also allows for in vitro studies on how primary human kidney cells regenerate renal structures after organ disruption.
Affiliation: Institute of Bioengineering and Nanotechnology, The Nanos, Singapore, Singapore.Show MeSH
Related in: MedlinePlus
Mentions: Cell movements involved in monolayer reorganization and tubule formation were not only highly coordinated, but also rapid. Figure 2 shows that condensation and folding up of the cell stripe, which ultimately led to tubule formation, occurred within minutes. We were not able to determine definitively how the tubule was folded from the stripe. This would require extensive live cell tracking and detailed analyses of fast movements of large numbers of cells. Such extensive live cell analyses would be performed in a separate study.
Affiliation: Institute of Bioengineering and Nanotechnology, The Nanos, Singapore, Singapore.