Generation of easily accessible human kidney tubules on two-dimensional surfaces in vitro.
Bottom Line: However, after triggering the process, the formation of renal tubules occurs with remarkable independence from the substrate architecture.Human proximal tubules generated on 2D surfaces typically have a length of several millimetres, and are easily accessible for manipulations and imaging, which makes them attractive for basic research and in vitro nephrotoxicology.The experimental system described also allows for in vitro studies on how primary human kidney cells regenerate renal structures after organ disruption.
Affiliation: Institute of Bioengineering and Nanotechnology, The Nanos, Singapore, Singapore.Show MeSH
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Mentions: In accordance with our previous results, we observed initial formation of a flat and well-differentiated epithelial monolayer when HPTCs were cultured in multiwell plates (Fig. 1A). Subsequently, increasing amounts of α-SMA-expressing myofibroblasts appeared. These myofibroblasts formed large aggregates (Fig. 1B). In the surroundings of such aggregates, the epithelium became reorganized. Firstly, highly coordinated and simultaneously directed movements of large numbers of cells led to retraction of the monolayer on one side of myofibroblast aggregates, leaving behind the largely empty surface of the well (Fig. 1C, E). Subsequently, the monolayer retracted on the other side of the myofibroblast aggregates (Fig. 1D, F). These highly coordinated cell movements led to the formation of stripes of cells, with a length of up to several millimetres or even centimetres (Fig. 1F). The stripes included the myofibroblast aggregates.
Affiliation: Institute of Bioengineering and Nanotechnology, The Nanos, Singapore, Singapore.