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Why is the partial oxygen pressure of human tissues a crucial parameter? Small molecules and hypoxia.

Carreau A, El Hafny-Rahbi B, Matejuk A, Grillon C, Kieda C - J. Cell. Mol. Med. (2011)

Bottom Line: The oxygen partial pressure (pO(2)), which is a key component of the physiological state of an organ, results from the balance between oxygen delivery and its consumption.More importantly we emphasize the discrepancy between in vivo and in vitro tissue and cells oxygen status which can have detrimental effects on experimental outcome.It is important to realize that most of the experiments performed in so-called normoxia might be dangerously misleading.

View Article: PubMed Central - PubMed

Affiliation: Centre de Biophysique Moléculaire, CNRS UPR, Orléans, France.

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Oxygen dependent modulation of angiogenin secretion by four distinct cell lines constituting the skin. Human skin endothelial microvascular cell line (HSkMEC), fibroblasts MSU 1.1, normal melanocyte cell line and HaCat keratinocytes have been maintained in normoxia, skin physioxia (22.8 mmHg or 3% O2) or hypoxia (less than 7.6 mmHg or 1% O2) during 16 hrs. Supernatants have been collected and angiogenin content assessed by the cytometric bead assay (CBA) technique. Results are expressed as picograms of angiogenin secreted by 10 × 6 cells in one hour (mean ± S.E.M.); *P < 0.05.
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fig08: Oxygen dependent modulation of angiogenin secretion by four distinct cell lines constituting the skin. Human skin endothelial microvascular cell line (HSkMEC), fibroblasts MSU 1.1, normal melanocyte cell line and HaCat keratinocytes have been maintained in normoxia, skin physioxia (22.8 mmHg or 3% O2) or hypoxia (less than 7.6 mmHg or 1% O2) during 16 hrs. Supernatants have been collected and angiogenin content assessed by the cytometric bead assay (CBA) technique. Results are expressed as picograms of angiogenin secreted by 10 × 6 cells in one hour (mean ± S.E.M.); *P < 0.05.

Mentions: As an example, angiogenin is up-regulated in various types of human cancers and takes part in tumour progression by stimulating both angiogenesis and cancer cell proliferation [90].The release of angiogenin from various cells present in the skin is very sensitive to oxygen pressure (Fig. 8). Strong hypoxia (less than 7.6 mmHg or 1% O2) induces an increase in angiogenin secretion for the cell lines derived from fibroblasts, human skin microvascular endothelial cells, normal melanocytes and keratinocytes. But skin physioxia (22.8 mmHg of O2 or 3% O2) is already able to lead to the same increase of angiogenin by keratinocytes and normal melanocyte cell line. On the opposite, this pO2 does not modify the angiogenin expression by human skin microvascular endothelial cell line or fibroblasts. Here again, results from normoxia does not reflect the real behaviour of cells in physioxia. Furthermore, this example points out the difference of sensitivity to oxygen pressure of distinct cell lines.


Why is the partial oxygen pressure of human tissues a crucial parameter? Small molecules and hypoxia.

Carreau A, El Hafny-Rahbi B, Matejuk A, Grillon C, Kieda C - J. Cell. Mol. Med. (2011)

Oxygen dependent modulation of angiogenin secretion by four distinct cell lines constituting the skin. Human skin endothelial microvascular cell line (HSkMEC), fibroblasts MSU 1.1, normal melanocyte cell line and HaCat keratinocytes have been maintained in normoxia, skin physioxia (22.8 mmHg or 3% O2) or hypoxia (less than 7.6 mmHg or 1% O2) during 16 hrs. Supernatants have been collected and angiogenin content assessed by the cytometric bead assay (CBA) technique. Results are expressed as picograms of angiogenin secreted by 10 × 6 cells in one hour (mean ± S.E.M.); *P < 0.05.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373326&req=5

fig08: Oxygen dependent modulation of angiogenin secretion by four distinct cell lines constituting the skin. Human skin endothelial microvascular cell line (HSkMEC), fibroblasts MSU 1.1, normal melanocyte cell line and HaCat keratinocytes have been maintained in normoxia, skin physioxia (22.8 mmHg or 3% O2) or hypoxia (less than 7.6 mmHg or 1% O2) during 16 hrs. Supernatants have been collected and angiogenin content assessed by the cytometric bead assay (CBA) technique. Results are expressed as picograms of angiogenin secreted by 10 × 6 cells in one hour (mean ± S.E.M.); *P < 0.05.
Mentions: As an example, angiogenin is up-regulated in various types of human cancers and takes part in tumour progression by stimulating both angiogenesis and cancer cell proliferation [90].The release of angiogenin from various cells present in the skin is very sensitive to oxygen pressure (Fig. 8). Strong hypoxia (less than 7.6 mmHg or 1% O2) induces an increase in angiogenin secretion for the cell lines derived from fibroblasts, human skin microvascular endothelial cells, normal melanocytes and keratinocytes. But skin physioxia (22.8 mmHg of O2 or 3% O2) is already able to lead to the same increase of angiogenin by keratinocytes and normal melanocyte cell line. On the opposite, this pO2 does not modify the angiogenin expression by human skin microvascular endothelial cell line or fibroblasts. Here again, results from normoxia does not reflect the real behaviour of cells in physioxia. Furthermore, this example points out the difference of sensitivity to oxygen pressure of distinct cell lines.

Bottom Line: The oxygen partial pressure (pO(2)), which is a key component of the physiological state of an organ, results from the balance between oxygen delivery and its consumption.More importantly we emphasize the discrepancy between in vivo and in vitro tissue and cells oxygen status which can have detrimental effects on experimental outcome.It is important to realize that most of the experiments performed in so-called normoxia might be dangerously misleading.

View Article: PubMed Central - PubMed

Affiliation: Centre de Biophysique Moléculaire, CNRS UPR, Orléans, France.

Show MeSH
Related in: MedlinePlus