Why is the partial oxygen pressure of human tissues a crucial parameter? Small molecules and hypoxia.
Bottom Line: The oxygen partial pressure (pO(2)), which is a key component of the physiological state of an organ, results from the balance between oxygen delivery and its consumption.More importantly we emphasize the discrepancy between in vivo and in vitro tissue and cells oxygen status which can have detrimental effects on experimental outcome.It is important to realize that most of the experiments performed in so-called normoxia might be dangerously misleading.
Affiliation: Centre de Biophysique Moléculaire, CNRS UPR, Orléans, France.Show MeSH
Related in: MedlinePlus
Mentions: Recently, an optic fibre-based system has been developed based on pO2-dependent changes in lifetime of the pulse of a fluorescent dye placed at the tip of a probe. The most commonly used fluorescent dye is ruthenium chloride. The oxygen in tissue or fluids quenches the light emitted by ruthenium; the light quenching is proportional to oxygen tension in the vicinity of the dye. Oxygen is not consumed during the reaction, so a continuous readout of tissue pO2 can be obtained . An example of pO2 measurement is presented in Figure 4.
Affiliation: Centre de Biophysique Moléculaire, CNRS UPR, Orléans, France.