Why is the partial oxygen pressure of human tissues a crucial parameter? Small molecules and hypoxia.
Bottom Line: The oxygen partial pressure (pO(2)), which is a key component of the physiological state of an organ, results from the balance between oxygen delivery and its consumption.More importantly we emphasize the discrepancy between in vivo and in vitro tissue and cells oxygen status which can have detrimental effects on experimental outcome.It is important to realize that most of the experiments performed in so-called normoxia might be dangerously misleading.
Affiliation: Centre de Biophysique Moléculaire, CNRS UPR, Orléans, France.Show MeSH
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Mentions: MRS is an application of MRI hereby spectra of metabolic changes in living tissue are obtained. In 1988, Busse et al.  showed that MRI could be used for imaging tumour pO2in vivo, after intravenous injection of perfluorocarbon (PFC) probes. This technique is referred as 19F-MRI. Mason et al.  used hexafluorobenzene because it gives a single, narrow 19F-MRI signal and is highly sensitive to oxygen concentration compared to the rest of PFCs. In fact, the 19F spin lattice relaxation rate (R1) of PFCs varies linearly with the dissolved oxygen concentration; thus, the 19F-based oximetry reports absolute values of oxygen concentration . An example of monitoring hypoxia of rat tumour is presented in Figure 3.
Affiliation: Centre de Biophysique Moléculaire, CNRS UPR, Orléans, France.