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Why is the partial oxygen pressure of human tissues a crucial parameter? Small molecules and hypoxia.

Carreau A, El Hafny-Rahbi B, Matejuk A, Grillon C, Kieda C - J. Cell. Mol. Med. (2011)

Bottom Line: The oxygen partial pressure (pO(2)), which is a key component of the physiological state of an organ, results from the balance between oxygen delivery and its consumption.More importantly we emphasize the discrepancy between in vivo and in vitro tissue and cells oxygen status which can have detrimental effects on experimental outcome.It is important to realize that most of the experiments performed in so-called normoxia might be dangerously misleading.

View Article: PubMed Central - PubMed

Affiliation: Centre de Biophysique Moléculaire, CNRS UPR, Orléans, France.

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Related in: MedlinePlus

Principles of PET. 18F radiotracer is intravenously injected. The tracer decays by emitting a positron, which annihilates with a nearby electron to produce two γ-rays. The PET scanner can detect the coincident γ-rays, and images can be reconstructed showing the location(s) and concentration of the tracer of interest. Sectional PET image is shown: normal uptake in brain (Br) and myocardium (C), and renal excretion into the urinary bladder (B) are visible. Also seen is a tumour (T) in the lungs that takes up more 18F radiotracer than the surrounding tissues. Adapted with permission from Macmillan Publishers Ltd: Nature Reviews Cancer, Gambhir, copyright 2002[5].
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fig02: Principles of PET. 18F radiotracer is intravenously injected. The tracer decays by emitting a positron, which annihilates with a nearby electron to produce two γ-rays. The PET scanner can detect the coincident γ-rays, and images can be reconstructed showing the location(s) and concentration of the tracer of interest. Sectional PET image is shown: normal uptake in brain (Br) and myocardium (C), and renal excretion into the urinary bladder (B) are visible. Also seen is a tumour (T) in the lungs that takes up more 18F radiotracer than the surrounding tissues. Adapted with permission from Macmillan Publishers Ltd: Nature Reviews Cancer, Gambhir, copyright 2002[5].

Mentions: PET is an in vivo tomographic imaging technique. It is based on the detection of anti-parallel 511 keV photons emitted during the annihilation of positrons with electrons. PET radiotracers are physiologically and pharmacologically relevant compounds labelled with short-lived positron-emitting radionuclides. After internalization by injection or inhalation, the tracer reaches the target and the quantity is then detected with a PET scanner, with a ring-shaped array of photoelectric crystals [5] (Fig. 2).


Why is the partial oxygen pressure of human tissues a crucial parameter? Small molecules and hypoxia.

Carreau A, El Hafny-Rahbi B, Matejuk A, Grillon C, Kieda C - J. Cell. Mol. Med. (2011)

Principles of PET. 18F radiotracer is intravenously injected. The tracer decays by emitting a positron, which annihilates with a nearby electron to produce two γ-rays. The PET scanner can detect the coincident γ-rays, and images can be reconstructed showing the location(s) and concentration of the tracer of interest. Sectional PET image is shown: normal uptake in brain (Br) and myocardium (C), and renal excretion into the urinary bladder (B) are visible. Also seen is a tumour (T) in the lungs that takes up more 18F radiotracer than the surrounding tissues. Adapted with permission from Macmillan Publishers Ltd: Nature Reviews Cancer, Gambhir, copyright 2002[5].
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373326&req=5

fig02: Principles of PET. 18F radiotracer is intravenously injected. The tracer decays by emitting a positron, which annihilates with a nearby electron to produce two γ-rays. The PET scanner can detect the coincident γ-rays, and images can be reconstructed showing the location(s) and concentration of the tracer of interest. Sectional PET image is shown: normal uptake in brain (Br) and myocardium (C), and renal excretion into the urinary bladder (B) are visible. Also seen is a tumour (T) in the lungs that takes up more 18F radiotracer than the surrounding tissues. Adapted with permission from Macmillan Publishers Ltd: Nature Reviews Cancer, Gambhir, copyright 2002[5].
Mentions: PET is an in vivo tomographic imaging technique. It is based on the detection of anti-parallel 511 keV photons emitted during the annihilation of positrons with electrons. PET radiotracers are physiologically and pharmacologically relevant compounds labelled with short-lived positron-emitting radionuclides. After internalization by injection or inhalation, the tracer reaches the target and the quantity is then detected with a PET scanner, with a ring-shaped array of photoelectric crystals [5] (Fig. 2).

Bottom Line: The oxygen partial pressure (pO(2)), which is a key component of the physiological state of an organ, results from the balance between oxygen delivery and its consumption.More importantly we emphasize the discrepancy between in vivo and in vitro tissue and cells oxygen status which can have detrimental effects on experimental outcome.It is important to realize that most of the experiments performed in so-called normoxia might be dangerously misleading.

View Article: PubMed Central - PubMed

Affiliation: Centre de Biophysique Moléculaire, CNRS UPR, Orléans, France.

Show MeSH
Related in: MedlinePlus