Directly auto-transplanted mesenchymal stem cells induce bone formation in a ceramic bone substitute in an ectopic sheep model.
Bottom Line: Bone matrix proteins were up-regulated in constructs following direct auto-transplantation and in expanded MSC as well as in BMP-2 constructs.Up-regulation was detected using immunohistology methods and RT-PCR.Dense vascularization was demonstrated by CD31 immunohistology staining in all three groups.
Affiliation: Department of Plastic and Hand Surgery, University Hospital of Erlangen, Friedrich-Alexander-University of Erlangen-Nürnberg, Erlangen, Germany.Show MeSH
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Mentions: MSC in a fibrinogen–thrombin matrix were implanted subcutaneously on the sheep’s back. Explants were harvested after 2 days, 1, 2, 4, 6 and 8 weeks to investigate proliferation, apoptosis and sufficient DiI labelling of the implanted MSC (groups 1–3). MSC DiI labelling was effective and was stable over several passages in cell culture (Fig. 2). DiI+ cells were identified at all time-points in the subcutaneous implants. The staining intensity of expanded MSC was stronger in comparison to directly auto-transplanted MSC.
Affiliation: Department of Plastic and Hand Surgery, University Hospital of Erlangen, Friedrich-Alexander-University of Erlangen-Nürnberg, Erlangen, Germany.