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Directly auto-transplanted mesenchymal stem cells induce bone formation in a ceramic bone substitute in an ectopic sheep model.

Boos AM, Loew JS, Deschler G, Arkudas A, Bleiziffer O, Gulle H, Dragu A, Kneser U, Horch RE, Beier JP - J. Cell. Mol. Med. (2010)

Bottom Line: Bone matrix proteins were up-regulated in constructs following direct auto-transplantation and in expanded MSC as well as in BMP-2 constructs.Up-regulation was detected using immunohistology methods and RT-PCR.Dense vascularization was demonstrated by CD31 immunohistology staining in all three groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic and Hand Surgery, University Hospital of Erlangen, Friedrich-Alexander-University of Erlangen-Nürnberg, Erlangen, Germany.

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DiI-labelled MSC at passage 5. MSC DiI labelling was effective and was stable over several passages in cell culture. Nuclei are counterstained with DAPI (blue).
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fig02: DiI-labelled MSC at passage 5. MSC DiI labelling was effective and was stable over several passages in cell culture. Nuclei are counterstained with DAPI (blue).

Mentions: MSC in a fibrinogen–thrombin matrix were implanted subcutaneously on the sheep’s back. Explants were harvested after 2 days, 1, 2, 4, 6 and 8 weeks to investigate proliferation, apoptosis and sufficient DiI labelling of the implanted MSC (groups 1–3). MSC DiI labelling was effective and was stable over several passages in cell culture (Fig. 2). DiI+ cells were identified at all time-points in the subcutaneous implants. The staining intensity of expanded MSC was stronger in comparison to directly auto-transplanted MSC.


Directly auto-transplanted mesenchymal stem cells induce bone formation in a ceramic bone substitute in an ectopic sheep model.

Boos AM, Loew JS, Deschler G, Arkudas A, Bleiziffer O, Gulle H, Dragu A, Kneser U, Horch RE, Beier JP - J. Cell. Mol. Med. (2010)

DiI-labelled MSC at passage 5. MSC DiI labelling was effective and was stable over several passages in cell culture. Nuclei are counterstained with DAPI (blue).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4373324&req=5

fig02: DiI-labelled MSC at passage 5. MSC DiI labelling was effective and was stable over several passages in cell culture. Nuclei are counterstained with DAPI (blue).
Mentions: MSC in a fibrinogen–thrombin matrix were implanted subcutaneously on the sheep’s back. Explants were harvested after 2 days, 1, 2, 4, 6 and 8 weeks to investigate proliferation, apoptosis and sufficient DiI labelling of the implanted MSC (groups 1–3). MSC DiI labelling was effective and was stable over several passages in cell culture (Fig. 2). DiI+ cells were identified at all time-points in the subcutaneous implants. The staining intensity of expanded MSC was stronger in comparison to directly auto-transplanted MSC.

Bottom Line: Bone matrix proteins were up-regulated in constructs following direct auto-transplantation and in expanded MSC as well as in BMP-2 constructs.Up-regulation was detected using immunohistology methods and RT-PCR.Dense vascularization was demonstrated by CD31 immunohistology staining in all three groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic and Hand Surgery, University Hospital of Erlangen, Friedrich-Alexander-University of Erlangen-Nürnberg, Erlangen, Germany.

Show MeSH
Related in: MedlinePlus