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Mesenchymal stem cells in rabbit meniscus and bone marrow exhibit a similar feature but a heterogeneous multi-differentiation potential: superiority of meniscus as a cell source for meniscus repair.

Ding Z, Huang H - BMC Musculoskelet Disord (2015)

Bottom Line: Finally, MMSCs always appeared a pronounced tendency to chondrogenic differentiation while BMSCs exhibited significantly greater osteogenic potential, whatever in vitro and in vivo.This study shows the similarities and differences between MMSCs and BMSCs for the first time.MMSCs are a promising source of mesenchymal stem cells in repairing meniscus defect.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, The 3rd Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, 1 Jinling Road, Nanjing, Jiangsu, 210001, China. dingzhe75@163.com.

ABSTRACT

Background: The restoration of damaged meniscus has always been a challenge due to its limited healing capacity. Recently, bone marrow-derived mesenchymal stem cells (BMSCs) provide a promising alternative to repair meniscal defects. However, BMSCs are not ideal chondroprogenitor cells for meniscus repair because they have a high propensity for cartilage hypertrophy and bone formation. Our hypothesis is that mesenchymal stem cells (MSCs) reside in meniscus maintain specific traits distinct from others which may be more conducive to meniscus regeneration.

Methods: MSCs were isolated from bone marrow and menisci of the rabbits. The similarities and differences between BMSCs and MMSCs were investigated in vitro by a cell culture model, ex vivo by a rabbit meniscus defect model and in vivo by a nude rat implantation model using histochemistry, immunocytochemistry, qRT-PCR and western blotting.

Results: Our data showed that two types of MSCs have universal stem cell characteristics including clonogenicity, multi-potency and self-renewal capacity. They both express stem cell markers including SSEA-4, Nanog, nucleostemin, strol-1, CD44 and CD90. However, MMSCs differed from BMSCs. MMSC colonies were much smaller and grew more slowly than BMSC colonies. Moreover, fewer MMSCs expressed CD34 than BMSCs. Finally, MMSCs always appeared a pronounced tendency to chondrogenic differentiation while BMSCs exhibited significantly greater osteogenic potential, whatever in vitro and in vivo.

Conclusions: This study shows the similarities and differences between MMSCs and BMSCs for the first time. MMSCs are a promising source of mesenchymal stem cells in repairing meniscus defect.

No MeSH data available.


Related in: MedlinePlus

Immunocytochemistry staining on complexes implanted Matrigel with BMSCs or MMSCs into the back of nude rats for 3 weeks. The tissue sections were stained with anti-adiponectin (A, B), anti-osteocalcin (C, D), anti-collagen type II (E, F), respectively. It is seen that two kinds of stem cells have multi-differentiation potential in vivo. The implantation of BMSCs resulted in more bone-like tissue formation (C, pink) than MMSCs (D). More cartilage-like tissues were observed in MMSCs group, as shown by immunostaining for collagen type II (F, red). (P < 0.05) (Magnification of microscopy: 20×) (Bar: 50 μm).
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Fig8: Immunocytochemistry staining on complexes implanted Matrigel with BMSCs or MMSCs into the back of nude rats for 3 weeks. The tissue sections were stained with anti-adiponectin (A, B), anti-osteocalcin (C, D), anti-collagen type II (E, F), respectively. It is seen that two kinds of stem cells have multi-differentiation potential in vivo. The implantation of BMSCs resulted in more bone-like tissue formation (C, pink) than MMSCs (D). More cartilage-like tissues were observed in MMSCs group, as shown by immunostaining for collagen type II (F, red). (P < 0.05) (Magnification of microscopy: 20×) (Bar: 50 μm).

Mentions: The differentiation capability in vivo was examined by implanting these two kinds of MSCs into nude rats. The results obtained from experiments showed that when MMSCs and BMSCs were subcutaneously implanted with Matrigel into the back of nude rats, in samples implanted with Matrigel-MSCs, greater positively for osteocalcin was observed in the samples made of Matrigel-BMSCs (Figure 8C) compared to those with Matrigel-MMSCs (Figure 8D), while the samples with Matrigel-MMSCs expressed much more collagen type II protein (Figure 8F) than those with Matrigel-BMSCs (Figure 8E). As for expression of adipogenesis, no significant difference were found between BMSCs and MMSCs.Figure 8


Mesenchymal stem cells in rabbit meniscus and bone marrow exhibit a similar feature but a heterogeneous multi-differentiation potential: superiority of meniscus as a cell source for meniscus repair.

Ding Z, Huang H - BMC Musculoskelet Disord (2015)

Immunocytochemistry staining on complexes implanted Matrigel with BMSCs or MMSCs into the back of nude rats for 3 weeks. The tissue sections were stained with anti-adiponectin (A, B), anti-osteocalcin (C, D), anti-collagen type II (E, F), respectively. It is seen that two kinds of stem cells have multi-differentiation potential in vivo. The implantation of BMSCs resulted in more bone-like tissue formation (C, pink) than MMSCs (D). More cartilage-like tissues were observed in MMSCs group, as shown by immunostaining for collagen type II (F, red). (P < 0.05) (Magnification of microscopy: 20×) (Bar: 50 μm).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4373281&req=5

Fig8: Immunocytochemistry staining on complexes implanted Matrigel with BMSCs or MMSCs into the back of nude rats for 3 weeks. The tissue sections were stained with anti-adiponectin (A, B), anti-osteocalcin (C, D), anti-collagen type II (E, F), respectively. It is seen that two kinds of stem cells have multi-differentiation potential in vivo. The implantation of BMSCs resulted in more bone-like tissue formation (C, pink) than MMSCs (D). More cartilage-like tissues were observed in MMSCs group, as shown by immunostaining for collagen type II (F, red). (P < 0.05) (Magnification of microscopy: 20×) (Bar: 50 μm).
Mentions: The differentiation capability in vivo was examined by implanting these two kinds of MSCs into nude rats. The results obtained from experiments showed that when MMSCs and BMSCs were subcutaneously implanted with Matrigel into the back of nude rats, in samples implanted with Matrigel-MSCs, greater positively for osteocalcin was observed in the samples made of Matrigel-BMSCs (Figure 8C) compared to those with Matrigel-MMSCs (Figure 8D), while the samples with Matrigel-MMSCs expressed much more collagen type II protein (Figure 8F) than those with Matrigel-BMSCs (Figure 8E). As for expression of adipogenesis, no significant difference were found between BMSCs and MMSCs.Figure 8

Bottom Line: Finally, MMSCs always appeared a pronounced tendency to chondrogenic differentiation while BMSCs exhibited significantly greater osteogenic potential, whatever in vitro and in vivo.This study shows the similarities and differences between MMSCs and BMSCs for the first time.MMSCs are a promising source of mesenchymal stem cells in repairing meniscus defect.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, The 3rd Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, 1 Jinling Road, Nanjing, Jiangsu, 210001, China. dingzhe75@163.com.

ABSTRACT

Background: The restoration of damaged meniscus has always been a challenge due to its limited healing capacity. Recently, bone marrow-derived mesenchymal stem cells (BMSCs) provide a promising alternative to repair meniscal defects. However, BMSCs are not ideal chondroprogenitor cells for meniscus repair because they have a high propensity for cartilage hypertrophy and bone formation. Our hypothesis is that mesenchymal stem cells (MSCs) reside in meniscus maintain specific traits distinct from others which may be more conducive to meniscus regeneration.

Methods: MSCs were isolated from bone marrow and menisci of the rabbits. The similarities and differences between BMSCs and MMSCs were investigated in vitro by a cell culture model, ex vivo by a rabbit meniscus defect model and in vivo by a nude rat implantation model using histochemistry, immunocytochemistry, qRT-PCR and western blotting.

Results: Our data showed that two types of MSCs have universal stem cell characteristics including clonogenicity, multi-potency and self-renewal capacity. They both express stem cell markers including SSEA-4, Nanog, nucleostemin, strol-1, CD44 and CD90. However, MMSCs differed from BMSCs. MMSC colonies were much smaller and grew more slowly than BMSC colonies. Moreover, fewer MMSCs expressed CD34 than BMSCs. Finally, MMSCs always appeared a pronounced tendency to chondrogenic differentiation while BMSCs exhibited significantly greater osteogenic potential, whatever in vitro and in vivo.

Conclusions: This study shows the similarities and differences between MMSCs and BMSCs for the first time. MMSCs are a promising source of mesenchymal stem cells in repairing meniscus defect.

No MeSH data available.


Related in: MedlinePlus