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Mesenchymal stem cells in rabbit meniscus and bone marrow exhibit a similar feature but a heterogeneous multi-differentiation potential: superiority of meniscus as a cell source for meniscus repair.

Ding Z, Huang H - BMC Musculoskelet Disord (2015)

Bottom Line: Finally, MMSCs always appeared a pronounced tendency to chondrogenic differentiation while BMSCs exhibited significantly greater osteogenic potential, whatever in vitro and in vivo.This study shows the similarities and differences between MMSCs and BMSCs for the first time.MMSCs are a promising source of mesenchymal stem cells in repairing meniscus defect.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, The 3rd Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, 1 Jinling Road, Nanjing, Jiangsu, 210001, China. dingzhe75@163.com.

ABSTRACT

Background: The restoration of damaged meniscus has always been a challenge due to its limited healing capacity. Recently, bone marrow-derived mesenchymal stem cells (BMSCs) provide a promising alternative to repair meniscal defects. However, BMSCs are not ideal chondroprogenitor cells for meniscus repair because they have a high propensity for cartilage hypertrophy and bone formation. Our hypothesis is that mesenchymal stem cells (MSCs) reside in meniscus maintain specific traits distinct from others which may be more conducive to meniscus regeneration.

Methods: MSCs were isolated from bone marrow and menisci of the rabbits. The similarities and differences between BMSCs and MMSCs were investigated in vitro by a cell culture model, ex vivo by a rabbit meniscus defect model and in vivo by a nude rat implantation model using histochemistry, immunocytochemistry, qRT-PCR and western blotting.

Results: Our data showed that two types of MSCs have universal stem cell characteristics including clonogenicity, multi-potency and self-renewal capacity. They both express stem cell markers including SSEA-4, Nanog, nucleostemin, strol-1, CD44 and CD90. However, MMSCs differed from BMSCs. MMSC colonies were much smaller and grew more slowly than BMSC colonies. Moreover, fewer MMSCs expressed CD34 than BMSCs. Finally, MMSCs always appeared a pronounced tendency to chondrogenic differentiation while BMSCs exhibited significantly greater osteogenic potential, whatever in vitro and in vivo.

Conclusions: This study shows the similarities and differences between MMSCs and BMSCs for the first time. MMSCs are a promising source of mesenchymal stem cells in repairing meniscus defect.

No MeSH data available.


Related in: MedlinePlus

Histochemical staining on wounded meniscus cultured with BMSCs or MMSCs for 6 weeks. The meniscus was cut into 10 μm section and stained by toluidine blue (A, B), safranin O (C, D), fast green and safranin O (E, F), alcian blue (G, H). All imaging showed that more cartilage-related proteins were detected in the meniscus treated with MMSCs than BMSCs. (P < 0.05) (Magnification of microscopy: 20×) (Bar: 50 μm).
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Fig7: Histochemical staining on wounded meniscus cultured with BMSCs or MMSCs for 6 weeks. The meniscus was cut into 10 μm section and stained by toluidine blue (A, B), safranin O (C, D), fast green and safranin O (E, F), alcian blue (G, H). All imaging showed that more cartilage-related proteins were detected in the meniscus treated with MMSCs than BMSCs. (P < 0.05) (Magnification of microscopy: 20×) (Bar: 50 μm).

Mentions: After 6 weeks culture, more than 90% of the wound area in rabbit meniscus was healed by MMSCs treatment; instead, by means of BMSCs treatment, 80% only was healed. Furthermore, more cartilage-related proteins were formed in the meniscus treated by MMSCs than that treated by BMSCs, as observed with staining using toluidine blue (dark brown in Figure 7B), safranin O (larger red area in Figure 7D), fast green and safranin O (larger red area in Figure 7F) and alcian blue (extensive green area in Figure 7H).Figure 7


Mesenchymal stem cells in rabbit meniscus and bone marrow exhibit a similar feature but a heterogeneous multi-differentiation potential: superiority of meniscus as a cell source for meniscus repair.

Ding Z, Huang H - BMC Musculoskelet Disord (2015)

Histochemical staining on wounded meniscus cultured with BMSCs or MMSCs for 6 weeks. The meniscus was cut into 10 μm section and stained by toluidine blue (A, B), safranin O (C, D), fast green and safranin O (E, F), alcian blue (G, H). All imaging showed that more cartilage-related proteins were detected in the meniscus treated with MMSCs than BMSCs. (P < 0.05) (Magnification of microscopy: 20×) (Bar: 50 μm).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4373281&req=5

Fig7: Histochemical staining on wounded meniscus cultured with BMSCs or MMSCs for 6 weeks. The meniscus was cut into 10 μm section and stained by toluidine blue (A, B), safranin O (C, D), fast green and safranin O (E, F), alcian blue (G, H). All imaging showed that more cartilage-related proteins were detected in the meniscus treated with MMSCs than BMSCs. (P < 0.05) (Magnification of microscopy: 20×) (Bar: 50 μm).
Mentions: After 6 weeks culture, more than 90% of the wound area in rabbit meniscus was healed by MMSCs treatment; instead, by means of BMSCs treatment, 80% only was healed. Furthermore, more cartilage-related proteins were formed in the meniscus treated by MMSCs than that treated by BMSCs, as observed with staining using toluidine blue (dark brown in Figure 7B), safranin O (larger red area in Figure 7D), fast green and safranin O (larger red area in Figure 7F) and alcian blue (extensive green area in Figure 7H).Figure 7

Bottom Line: Finally, MMSCs always appeared a pronounced tendency to chondrogenic differentiation while BMSCs exhibited significantly greater osteogenic potential, whatever in vitro and in vivo.This study shows the similarities and differences between MMSCs and BMSCs for the first time.MMSCs are a promising source of mesenchymal stem cells in repairing meniscus defect.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, The 3rd Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, 1 Jinling Road, Nanjing, Jiangsu, 210001, China. dingzhe75@163.com.

ABSTRACT

Background: The restoration of damaged meniscus has always been a challenge due to its limited healing capacity. Recently, bone marrow-derived mesenchymal stem cells (BMSCs) provide a promising alternative to repair meniscal defects. However, BMSCs are not ideal chondroprogenitor cells for meniscus repair because they have a high propensity for cartilage hypertrophy and bone formation. Our hypothesis is that mesenchymal stem cells (MSCs) reside in meniscus maintain specific traits distinct from others which may be more conducive to meniscus regeneration.

Methods: MSCs were isolated from bone marrow and menisci of the rabbits. The similarities and differences between BMSCs and MMSCs were investigated in vitro by a cell culture model, ex vivo by a rabbit meniscus defect model and in vivo by a nude rat implantation model using histochemistry, immunocytochemistry, qRT-PCR and western blotting.

Results: Our data showed that two types of MSCs have universal stem cell characteristics including clonogenicity, multi-potency and self-renewal capacity. They both express stem cell markers including SSEA-4, Nanog, nucleostemin, strol-1, CD44 and CD90. However, MMSCs differed from BMSCs. MMSC colonies were much smaller and grew more slowly than BMSC colonies. Moreover, fewer MMSCs expressed CD34 than BMSCs. Finally, MMSCs always appeared a pronounced tendency to chondrogenic differentiation while BMSCs exhibited significantly greater osteogenic potential, whatever in vitro and in vivo.

Conclusions: This study shows the similarities and differences between MMSCs and BMSCs for the first time. MMSCs are a promising source of mesenchymal stem cells in repairing meniscus defect.

No MeSH data available.


Related in: MedlinePlus