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Mesenchymal stem cells in rabbit meniscus and bone marrow exhibit a similar feature but a heterogeneous multi-differentiation potential: superiority of meniscus as a cell source for meniscus repair.

Ding Z, Huang H - BMC Musculoskelet Disord (2015)

Bottom Line: Finally, MMSCs always appeared a pronounced tendency to chondrogenic differentiation while BMSCs exhibited significantly greater osteogenic potential, whatever in vitro and in vivo.This study shows the similarities and differences between MMSCs and BMSCs for the first time.MMSCs are a promising source of mesenchymal stem cells in repairing meniscus defect.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, The 3rd Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, 1 Jinling Road, Nanjing, Jiangsu, 210001, China. dingzhe75@163.com.

ABSTRACT

Background: The restoration of damaged meniscus has always been a challenge due to its limited healing capacity. Recently, bone marrow-derived mesenchymal stem cells (BMSCs) provide a promising alternative to repair meniscal defects. However, BMSCs are not ideal chondroprogenitor cells for meniscus repair because they have a high propensity for cartilage hypertrophy and bone formation. Our hypothesis is that mesenchymal stem cells (MSCs) reside in meniscus maintain specific traits distinct from others which may be more conducive to meniscus regeneration.

Methods: MSCs were isolated from bone marrow and menisci of the rabbits. The similarities and differences between BMSCs and MMSCs were investigated in vitro by a cell culture model, ex vivo by a rabbit meniscus defect model and in vivo by a nude rat implantation model using histochemistry, immunocytochemistry, qRT-PCR and western blotting.

Results: Our data showed that two types of MSCs have universal stem cell characteristics including clonogenicity, multi-potency and self-renewal capacity. They both express stem cell markers including SSEA-4, Nanog, nucleostemin, strol-1, CD44 and CD90. However, MMSCs differed from BMSCs. MMSC colonies were much smaller and grew more slowly than BMSC colonies. Moreover, fewer MMSCs expressed CD34 than BMSCs. Finally, MMSCs always appeared a pronounced tendency to chondrogenic differentiation while BMSCs exhibited significantly greater osteogenic potential, whatever in vitro and in vivo.

Conclusions: This study shows the similarities and differences between MMSCs and BMSCs for the first time. MMSCs are a promising source of mesenchymal stem cells in repairing meniscus defect.

No MeSH data available.


Related in: MedlinePlus

Representative western blots of differentiated BMSCs and MMSCs. Collagen type II, the most important indicator for chondrogenesis, was expressed much higher in MMSCs than that in BMSCs. Meanwhile, BMSCs displayed higher expression of osteocalcin, a typical production of osteogenesis. Note that our data were normalized to GAPDH, and obtained from at least three independent experiments. (P < 0.05).
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Fig6: Representative western blots of differentiated BMSCs and MMSCs. Collagen type II, the most important indicator for chondrogenesis, was expressed much higher in MMSCs than that in BMSCs. Meanwhile, BMSCs displayed higher expression of osteocalcin, a typical production of osteogenesis. Note that our data were normalized to GAPDH, and obtained from at least three independent experiments. (P < 0.05).

Mentions: Western blotting was performed to quantify the level of specific protein expression in the two groups of stem cells. Adiponectin was expressed in both MMSCs and BMSCs following 21 days culture in adipogenic induction medium and there was no significant difference between two groups. As for osteocalcin, a well-known marker of osteogenesis, it was markedly up-regulated in BMSCs compared with MMSCs. Conversely, after cultured in chondrogenic medium, MMSCs expressed significantly higher levels of collagen type II which is one of the most important indicators on chondrogenesis than BMSCs (Figure 6).Figure 6


Mesenchymal stem cells in rabbit meniscus and bone marrow exhibit a similar feature but a heterogeneous multi-differentiation potential: superiority of meniscus as a cell source for meniscus repair.

Ding Z, Huang H - BMC Musculoskelet Disord (2015)

Representative western blots of differentiated BMSCs and MMSCs. Collagen type II, the most important indicator for chondrogenesis, was expressed much higher in MMSCs than that in BMSCs. Meanwhile, BMSCs displayed higher expression of osteocalcin, a typical production of osteogenesis. Note that our data were normalized to GAPDH, and obtained from at least three independent experiments. (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4373281&req=5

Fig6: Representative western blots of differentiated BMSCs and MMSCs. Collagen type II, the most important indicator for chondrogenesis, was expressed much higher in MMSCs than that in BMSCs. Meanwhile, BMSCs displayed higher expression of osteocalcin, a typical production of osteogenesis. Note that our data were normalized to GAPDH, and obtained from at least three independent experiments. (P < 0.05).
Mentions: Western blotting was performed to quantify the level of specific protein expression in the two groups of stem cells. Adiponectin was expressed in both MMSCs and BMSCs following 21 days culture in adipogenic induction medium and there was no significant difference between two groups. As for osteocalcin, a well-known marker of osteogenesis, it was markedly up-regulated in BMSCs compared with MMSCs. Conversely, after cultured in chondrogenic medium, MMSCs expressed significantly higher levels of collagen type II which is one of the most important indicators on chondrogenesis than BMSCs (Figure 6).Figure 6

Bottom Line: Finally, MMSCs always appeared a pronounced tendency to chondrogenic differentiation while BMSCs exhibited significantly greater osteogenic potential, whatever in vitro and in vivo.This study shows the similarities and differences between MMSCs and BMSCs for the first time.MMSCs are a promising source of mesenchymal stem cells in repairing meniscus defect.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, The 3rd Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, 1 Jinling Road, Nanjing, Jiangsu, 210001, China. dingzhe75@163.com.

ABSTRACT

Background: The restoration of damaged meniscus has always been a challenge due to its limited healing capacity. Recently, bone marrow-derived mesenchymal stem cells (BMSCs) provide a promising alternative to repair meniscal defects. However, BMSCs are not ideal chondroprogenitor cells for meniscus repair because they have a high propensity for cartilage hypertrophy and bone formation. Our hypothesis is that mesenchymal stem cells (MSCs) reside in meniscus maintain specific traits distinct from others which may be more conducive to meniscus regeneration.

Methods: MSCs were isolated from bone marrow and menisci of the rabbits. The similarities and differences between BMSCs and MMSCs were investigated in vitro by a cell culture model, ex vivo by a rabbit meniscus defect model and in vivo by a nude rat implantation model using histochemistry, immunocytochemistry, qRT-PCR and western blotting.

Results: Our data showed that two types of MSCs have universal stem cell characteristics including clonogenicity, multi-potency and self-renewal capacity. They both express stem cell markers including SSEA-4, Nanog, nucleostemin, strol-1, CD44 and CD90. However, MMSCs differed from BMSCs. MMSC colonies were much smaller and grew more slowly than BMSC colonies. Moreover, fewer MMSCs expressed CD34 than BMSCs. Finally, MMSCs always appeared a pronounced tendency to chondrogenic differentiation while BMSCs exhibited significantly greater osteogenic potential, whatever in vitro and in vivo.

Conclusions: This study shows the similarities and differences between MMSCs and BMSCs for the first time. MMSCs are a promising source of mesenchymal stem cells in repairing meniscus defect.

No MeSH data available.


Related in: MedlinePlus