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Mesenchymal stem cells in rabbit meniscus and bone marrow exhibit a similar feature but a heterogeneous multi-differentiation potential: superiority of meniscus as a cell source for meniscus repair.

Ding Z, Huang H - BMC Musculoskelet Disord (2015)

Bottom Line: Finally, MMSCs always appeared a pronounced tendency to chondrogenic differentiation while BMSCs exhibited significantly greater osteogenic potential, whatever in vitro and in vivo.This study shows the similarities and differences between MMSCs and BMSCs for the first time.MMSCs are a promising source of mesenchymal stem cells in repairing meniscus defect.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, The 3rd Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, 1 Jinling Road, Nanjing, Jiangsu, 210001, China. dingzhe75@163.com.

ABSTRACT

Background: The restoration of damaged meniscus has always been a challenge due to its limited healing capacity. Recently, bone marrow-derived mesenchymal stem cells (BMSCs) provide a promising alternative to repair meniscal defects. However, BMSCs are not ideal chondroprogenitor cells for meniscus repair because they have a high propensity for cartilage hypertrophy and bone formation. Our hypothesis is that mesenchymal stem cells (MSCs) reside in meniscus maintain specific traits distinct from others which may be more conducive to meniscus regeneration.

Methods: MSCs were isolated from bone marrow and menisci of the rabbits. The similarities and differences between BMSCs and MMSCs were investigated in vitro by a cell culture model, ex vivo by a rabbit meniscus defect model and in vivo by a nude rat implantation model using histochemistry, immunocytochemistry, qRT-PCR and western blotting.

Results: Our data showed that two types of MSCs have universal stem cell characteristics including clonogenicity, multi-potency and self-renewal capacity. They both express stem cell markers including SSEA-4, Nanog, nucleostemin, strol-1, CD44 and CD90. However, MMSCs differed from BMSCs. MMSC colonies were much smaller and grew more slowly than BMSC colonies. Moreover, fewer MMSCs expressed CD34 than BMSCs. Finally, MMSCs always appeared a pronounced tendency to chondrogenic differentiation while BMSCs exhibited significantly greater osteogenic potential, whatever in vitro and in vivo.

Conclusions: This study shows the similarities and differences between MMSCs and BMSCs for the first time. MMSCs are a promising source of mesenchymal stem cells in repairing meniscus defect.

No MeSH data available.


Related in: MedlinePlus

The colony formation of bone marrow-derived stem cells(BMSCs)and meniscus-derived stem cells(MMSCs).A. Colonies of BMSCs. B. Colonies of MMSCs. C. A sample colony of BMSCs. D. A sample colony of MMSCs. E. Quantitative analysis of colonies formed by BMSCs and MMSCs. The colonies were detected by staining with Methyl violet at 15 days primary culture. Cell numbers were counted after trypsinized from each colony respectively. Colony number of MMSCs was significantly different from that of BMSCs (*p < 0.05). (Bars: 50 μm).
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Fig1: The colony formation of bone marrow-derived stem cells(BMSCs)and meniscus-derived stem cells(MMSCs).A. Colonies of BMSCs. B. Colonies of MMSCs. C. A sample colony of BMSCs. D. A sample colony of MMSCs. E. Quantitative analysis of colonies formed by BMSCs and MMSCs. The colonies were detected by staining with Methyl violet at 15 days primary culture. Cell numbers were counted after trypsinized from each colony respectively. Colony number of MMSCs was significantly different from that of BMSCs (*p < 0.05). (Bars: 50 μm).

Mentions: To characterize whether meniscus-derived cells are clonogenic, we isolated and cultured single suspension from rabbit meniscus and compared with bone marrow-derived cells in five T25 flasks. During the initial three days in culture, these cells began to attach onto the plastic surface and remained quiescent for approximate five days. After 8–10 days of culture, the first colony was observed in each flask. Then large quantities of cells started rapidly dividing to form considerable colonies at 10–15 days. Methyl violet staining was used to discover the colony (Figure 1 A, B). However, the number and size of cell colonies from BMSCs and MMSCs were markedly different: colonies formed by MMSCs were fewer in number and smaller in size than those of BMSCs. Our data showed that only 51.8% of colonies consisted of 50,000 cells or more in MMSCs whereas 75.8% in BMSCs (Figure 1E).Figure 1


Mesenchymal stem cells in rabbit meniscus and bone marrow exhibit a similar feature but a heterogeneous multi-differentiation potential: superiority of meniscus as a cell source for meniscus repair.

Ding Z, Huang H - BMC Musculoskelet Disord (2015)

The colony formation of bone marrow-derived stem cells(BMSCs)and meniscus-derived stem cells(MMSCs).A. Colonies of BMSCs. B. Colonies of MMSCs. C. A sample colony of BMSCs. D. A sample colony of MMSCs. E. Quantitative analysis of colonies formed by BMSCs and MMSCs. The colonies were detected by staining with Methyl violet at 15 days primary culture. Cell numbers were counted after trypsinized from each colony respectively. Colony number of MMSCs was significantly different from that of BMSCs (*p < 0.05). (Bars: 50 μm).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4373281&req=5

Fig1: The colony formation of bone marrow-derived stem cells(BMSCs)and meniscus-derived stem cells(MMSCs).A. Colonies of BMSCs. B. Colonies of MMSCs. C. A sample colony of BMSCs. D. A sample colony of MMSCs. E. Quantitative analysis of colonies formed by BMSCs and MMSCs. The colonies were detected by staining with Methyl violet at 15 days primary culture. Cell numbers were counted after trypsinized from each colony respectively. Colony number of MMSCs was significantly different from that of BMSCs (*p < 0.05). (Bars: 50 μm).
Mentions: To characterize whether meniscus-derived cells are clonogenic, we isolated and cultured single suspension from rabbit meniscus and compared with bone marrow-derived cells in five T25 flasks. During the initial three days in culture, these cells began to attach onto the plastic surface and remained quiescent for approximate five days. After 8–10 days of culture, the first colony was observed in each flask. Then large quantities of cells started rapidly dividing to form considerable colonies at 10–15 days. Methyl violet staining was used to discover the colony (Figure 1 A, B). However, the number and size of cell colonies from BMSCs and MMSCs were markedly different: colonies formed by MMSCs were fewer in number and smaller in size than those of BMSCs. Our data showed that only 51.8% of colonies consisted of 50,000 cells or more in MMSCs whereas 75.8% in BMSCs (Figure 1E).Figure 1

Bottom Line: Finally, MMSCs always appeared a pronounced tendency to chondrogenic differentiation while BMSCs exhibited significantly greater osteogenic potential, whatever in vitro and in vivo.This study shows the similarities and differences between MMSCs and BMSCs for the first time.MMSCs are a promising source of mesenchymal stem cells in repairing meniscus defect.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, The 3rd Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, 1 Jinling Road, Nanjing, Jiangsu, 210001, China. dingzhe75@163.com.

ABSTRACT

Background: The restoration of damaged meniscus has always been a challenge due to its limited healing capacity. Recently, bone marrow-derived mesenchymal stem cells (BMSCs) provide a promising alternative to repair meniscal defects. However, BMSCs are not ideal chondroprogenitor cells for meniscus repair because they have a high propensity for cartilage hypertrophy and bone formation. Our hypothesis is that mesenchymal stem cells (MSCs) reside in meniscus maintain specific traits distinct from others which may be more conducive to meniscus regeneration.

Methods: MSCs were isolated from bone marrow and menisci of the rabbits. The similarities and differences between BMSCs and MMSCs were investigated in vitro by a cell culture model, ex vivo by a rabbit meniscus defect model and in vivo by a nude rat implantation model using histochemistry, immunocytochemistry, qRT-PCR and western blotting.

Results: Our data showed that two types of MSCs have universal stem cell characteristics including clonogenicity, multi-potency and self-renewal capacity. They both express stem cell markers including SSEA-4, Nanog, nucleostemin, strol-1, CD44 and CD90. However, MMSCs differed from BMSCs. MMSC colonies were much smaller and grew more slowly than BMSC colonies. Moreover, fewer MMSCs expressed CD34 than BMSCs. Finally, MMSCs always appeared a pronounced tendency to chondrogenic differentiation while BMSCs exhibited significantly greater osteogenic potential, whatever in vitro and in vivo.

Conclusions: This study shows the similarities and differences between MMSCs and BMSCs for the first time. MMSCs are a promising source of mesenchymal stem cells in repairing meniscus defect.

No MeSH data available.


Related in: MedlinePlus