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Detection of morphologic alterations in rectal carcinoma following preoperative radiochemotherapy based on multiphoton microscopy imaging.

Li L, Chen Z, Wang X, Li H, Jiang W, Zhuo S, Guan G, Chen J - BMC Cancer (2015)

Bottom Line: Preoperative radiochemotherapy improves outcomes in patients with locally advanced rectal carcinoma, and has been used increasingly in patient management.In this study, we used multiphoton microscopy (MPM) to detect morphologic alterations in rectal adenocarcinomas in patients treated with preoperative radiochemotherapy.MPM was able to identify histopathologic alterations in rectal cancer following preoperative radiochemotherapy, and allowed the qualitative assessment of treatment efficacy and feasibility in relation to dose or strategy.

View Article: PubMed Central - PubMed

Affiliation: Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, 350007, China. lhli@fjnu.edu.cn.

ABSTRACT

Background: Preoperative radiochemotherapy improves outcomes in patients with locally advanced rectal carcinoma, and has been used increasingly in patient management. However, there is a strong clinical need to assess tumor response to neoadjuvant treatment, and a non-invasive technique that allows the precise identification of morphologic changes in tumors would be of considerable clinical interest.

Methods: In this study, we used multiphoton microscopy (MPM) to detect morphologic alterations in rectal adenocarcinomas in patients treated with preoperative radiochemotherapy.

Results: MPM was able to identify histopathologic alterations in rectal cancer following preoperative radiochemotherapy, and allowed the qualitative assessment of treatment efficacy and feasibility in relation to dose or strategy.

Conclusion: These findings may provide the groundwork for evaluating tumor response to neoadjuvant treatment, thus allowing the tailoring of effective treatment doses and strategies.

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Related in: MedlinePlus

Representative TPEF/SHG images of blood vessel changes with thickening and fibrosis of the intima and media, and inflammatory cell infiltration. Scale bar‚ÄČ=‚ÄČ100¬†őľm. (a) SHG image (green); (b) TPEF image (red); (c) overlay of SHG/TPEF images; and (d) corresponding H&E-stained image (40√ó magnification).
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Fig2: Representative TPEF/SHG images of blood vessel changes with thickening and fibrosis of the intima and media, and inflammatory cell infiltration. Scale bar‚ÄČ=‚ÄČ100¬†őľm. (a) SHG image (green); (b) TPEF image (red); (c) overlay of SHG/TPEF images; and (d) corresponding H&E-stained image (40√ó magnification).

Mentions: MPM and H&E staining revealed obvious radiogenic blood vessel changes and inflammatory cell infiltration in the serosa after preoperative radiochemotherapy (Figure 2). The blood vessels showed significant alterations with thickening and fibrosis of the intima and media, as shown by TPEF signals in MPM (Figure 2(c)) (blue arrows) [7]. TPEF signals also revealed inflammatory cell infiltration and large numbers of inflammatory cells infiltrating into the stroma (Figure 2(c)) (white arrows). The tumor-associated inflammatory reaction has long been considered as a type of host response and an important factor in tumor progression [16]. Previous studies also demonstrated lower recurrence rates and better outcomes in patients with rectal cancer who had abundant inflammatory cells in the stroma post-irradiation [17,18]. These qualitative morphological variations were consistent with the paired histological sections in the current study (Figure 2(d)).Figure 2


Detection of morphologic alterations in rectal carcinoma following preoperative radiochemotherapy based on multiphoton microscopy imaging.

Li L, Chen Z, Wang X, Li H, Jiang W, Zhuo S, Guan G, Chen J - BMC Cancer (2015)

Representative TPEF/SHG images of blood vessel changes with thickening and fibrosis of the intima and media, and inflammatory cell infiltration. Scale bar‚ÄČ=‚ÄČ100¬†őľm. (a) SHG image (green); (b) TPEF image (red); (c) overlay of SHG/TPEF images; and (d) corresponding H&E-stained image (40√ó magnification).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4373096&req=5

Fig2: Representative TPEF/SHG images of blood vessel changes with thickening and fibrosis of the intima and media, and inflammatory cell infiltration. Scale bar‚ÄČ=‚ÄČ100¬†őľm. (a) SHG image (green); (b) TPEF image (red); (c) overlay of SHG/TPEF images; and (d) corresponding H&E-stained image (40√ó magnification).
Mentions: MPM and H&E staining revealed obvious radiogenic blood vessel changes and inflammatory cell infiltration in the serosa after preoperative radiochemotherapy (Figure 2). The blood vessels showed significant alterations with thickening and fibrosis of the intima and media, as shown by TPEF signals in MPM (Figure 2(c)) (blue arrows) [7]. TPEF signals also revealed inflammatory cell infiltration and large numbers of inflammatory cells infiltrating into the stroma (Figure 2(c)) (white arrows). The tumor-associated inflammatory reaction has long been considered as a type of host response and an important factor in tumor progression [16]. Previous studies also demonstrated lower recurrence rates and better outcomes in patients with rectal cancer who had abundant inflammatory cells in the stroma post-irradiation [17,18]. These qualitative morphological variations were consistent with the paired histological sections in the current study (Figure 2(d)).Figure 2

Bottom Line: Preoperative radiochemotherapy improves outcomes in patients with locally advanced rectal carcinoma, and has been used increasingly in patient management.In this study, we used multiphoton microscopy (MPM) to detect morphologic alterations in rectal adenocarcinomas in patients treated with preoperative radiochemotherapy.MPM was able to identify histopathologic alterations in rectal cancer following preoperative radiochemotherapy, and allowed the qualitative assessment of treatment efficacy and feasibility in relation to dose or strategy.

View Article: PubMed Central - PubMed

Affiliation: Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, 350007, China. lhli@fjnu.edu.cn.

ABSTRACT

Background: Preoperative radiochemotherapy improves outcomes in patients with locally advanced rectal carcinoma, and has been used increasingly in patient management. However, there is a strong clinical need to assess tumor response to neoadjuvant treatment, and a non-invasive technique that allows the precise identification of morphologic changes in tumors would be of considerable clinical interest.

Methods: In this study, we used multiphoton microscopy (MPM) to detect morphologic alterations in rectal adenocarcinomas in patients treated with preoperative radiochemotherapy.

Results: MPM was able to identify histopathologic alterations in rectal cancer following preoperative radiochemotherapy, and allowed the qualitative assessment of treatment efficacy and feasibility in relation to dose or strategy.

Conclusion: These findings may provide the groundwork for evaluating tumor response to neoadjuvant treatment, thus allowing the tailoring of effective treatment doses and strategies.

Show MeSH
Related in: MedlinePlus