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Detection of morphologic alterations in rectal carcinoma following preoperative radiochemotherapy based on multiphoton microscopy imaging.

Li L, Chen Z, Wang X, Li H, Jiang W, Zhuo S, Guan G, Chen J - BMC Cancer (2015)

Bottom Line: Preoperative radiochemotherapy improves outcomes in patients with locally advanced rectal carcinoma, and has been used increasingly in patient management.In this study, we used multiphoton microscopy (MPM) to detect morphologic alterations in rectal adenocarcinomas in patients treated with preoperative radiochemotherapy.MPM was able to identify histopathologic alterations in rectal cancer following preoperative radiochemotherapy, and allowed the qualitative assessment of treatment efficacy and feasibility in relation to dose or strategy.

View Article: PubMed Central - PubMed

Affiliation: Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, 350007, China. lhli@fjnu.edu.cn.

ABSTRACT

Background: Preoperative radiochemotherapy improves outcomes in patients with locally advanced rectal carcinoma, and has been used increasingly in patient management. However, there is a strong clinical need to assess tumor response to neoadjuvant treatment, and a non-invasive technique that allows the precise identification of morphologic changes in tumors would be of considerable clinical interest.

Methods: In this study, we used multiphoton microscopy (MPM) to detect morphologic alterations in rectal adenocarcinomas in patients treated with preoperative radiochemotherapy.

Results: MPM was able to identify histopathologic alterations in rectal cancer following preoperative radiochemotherapy, and allowed the qualitative assessment of treatment efficacy and feasibility in relation to dose or strategy.

Conclusion: These findings may provide the groundwork for evaluating tumor response to neoadjuvant treatment, thus allowing the tailoring of effective treatment doses and strategies.

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Related in: MedlinePlus

Representative TPEF/SHG images of stromal alterations in rectal carcinoma after preoperative radiochemotherapy characterized by fibrosis or fibroinflammatory changes. Scale bar = 100 μm. (a) SHG image (green); (b) TPEF image (red); (c) overlay of SHG/TPEF images; and (d) corresponding H&E-stained image (40× magnification).
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Fig1: Representative TPEF/SHG images of stromal alterations in rectal carcinoma after preoperative radiochemotherapy characterized by fibrosis or fibroinflammatory changes. Scale bar = 100 μm. (a) SHG image (green); (b) TPEF image (red); (c) overlay of SHG/TPEF images; and (d) corresponding H&E-stained image (40× magnification).

Mentions: Representative MPM and corresponding H&E-stained images showed stromal alterations in rectal carcinomas induced by preoperative radiochemotherapy with cancerous cell invasion into the muscularis propria (Figure 1). MPM images clearly revealed stromal changes in rectal cancer patients undergoing preoperative radiochemotherapy. Consecutive muscular tissues were disrupted and collagen fibers were abundant but disordered, as demonstrated by SHG signals (green) (Figure 1(a)). This may be interpreted as former tumor infiltration leading to the destruction of muscular tissues, and post-treatment tumor regression represented by fibrosis or fibroinflammatory changes replacing neoplastic glands [15,16]. These fibrotic tissues also produced comparable TPEF signals (red) (Figure 1(b)), and overlaid TPEF/SHG images therefore appear yellowish (Figure 1(c)). The details revealed by MPM correlated well with the H&E-stained images (Figure 1(d)).Figure 1


Detection of morphologic alterations in rectal carcinoma following preoperative radiochemotherapy based on multiphoton microscopy imaging.

Li L, Chen Z, Wang X, Li H, Jiang W, Zhuo S, Guan G, Chen J - BMC Cancer (2015)

Representative TPEF/SHG images of stromal alterations in rectal carcinoma after preoperative radiochemotherapy characterized by fibrosis or fibroinflammatory changes. Scale bar = 100 μm. (a) SHG image (green); (b) TPEF image (red); (c) overlay of SHG/TPEF images; and (d) corresponding H&E-stained image (40× magnification).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4373096&req=5

Fig1: Representative TPEF/SHG images of stromal alterations in rectal carcinoma after preoperative radiochemotherapy characterized by fibrosis or fibroinflammatory changes. Scale bar = 100 μm. (a) SHG image (green); (b) TPEF image (red); (c) overlay of SHG/TPEF images; and (d) corresponding H&E-stained image (40× magnification).
Mentions: Representative MPM and corresponding H&E-stained images showed stromal alterations in rectal carcinomas induced by preoperative radiochemotherapy with cancerous cell invasion into the muscularis propria (Figure 1). MPM images clearly revealed stromal changes in rectal cancer patients undergoing preoperative radiochemotherapy. Consecutive muscular tissues were disrupted and collagen fibers were abundant but disordered, as demonstrated by SHG signals (green) (Figure 1(a)). This may be interpreted as former tumor infiltration leading to the destruction of muscular tissues, and post-treatment tumor regression represented by fibrosis or fibroinflammatory changes replacing neoplastic glands [15,16]. These fibrotic tissues also produced comparable TPEF signals (red) (Figure 1(b)), and overlaid TPEF/SHG images therefore appear yellowish (Figure 1(c)). The details revealed by MPM correlated well with the H&E-stained images (Figure 1(d)).Figure 1

Bottom Line: Preoperative radiochemotherapy improves outcomes in patients with locally advanced rectal carcinoma, and has been used increasingly in patient management.In this study, we used multiphoton microscopy (MPM) to detect morphologic alterations in rectal adenocarcinomas in patients treated with preoperative radiochemotherapy.MPM was able to identify histopathologic alterations in rectal cancer following preoperative radiochemotherapy, and allowed the qualitative assessment of treatment efficacy and feasibility in relation to dose or strategy.

View Article: PubMed Central - PubMed

Affiliation: Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, 350007, China. lhli@fjnu.edu.cn.

ABSTRACT

Background: Preoperative radiochemotherapy improves outcomes in patients with locally advanced rectal carcinoma, and has been used increasingly in patient management. However, there is a strong clinical need to assess tumor response to neoadjuvant treatment, and a non-invasive technique that allows the precise identification of morphologic changes in tumors would be of considerable clinical interest.

Methods: In this study, we used multiphoton microscopy (MPM) to detect morphologic alterations in rectal adenocarcinomas in patients treated with preoperative radiochemotherapy.

Results: MPM was able to identify histopathologic alterations in rectal cancer following preoperative radiochemotherapy, and allowed the qualitative assessment of treatment efficacy and feasibility in relation to dose or strategy.

Conclusion: These findings may provide the groundwork for evaluating tumor response to neoadjuvant treatment, thus allowing the tailoring of effective treatment doses and strategies.

Show MeSH
Related in: MedlinePlus