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Feeding habits of mosquitoes (Diptera: Culicidae) in an area of sylvatic transmission of yellow fever in the state of São Paulo, Brazil.

Mucci LF, Júnior RP, de Paula MB, Scandar SA, Pacchioni ML, Fernandes A, Consales CA - J Venom Anim Toxins Incl Trop Dis (2015)

Bottom Line: Of the principal vector species, Haemagogus janthinomys/capricornii was found with bird, bovine and primate blood.The eclectic feeding habits and some aspects of the interactions between potential vectors and reservoirs of yellow fever may be associated with the habitat fragmentation characteristic of the region.We recommend that further studies on the capacity and vector competence be performed on secondary vectors in extra-Amazonian region.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biology and Ecology of Mosquitoes, Superintendence for Endemic Disease Control, State Health Secretariat, Taubaté, São Paulo State Brazil.

ABSTRACT

Background: The reintroduction of sylvatic yellow fever in the state of São Paulo after about six decades was confirmed in the Northwestern region in 2000, where in 2008 there also occurred an important epizootic. The purpose of this study was to investigate the feeding habits of culicids potentially involved in the sylvatic transmission of the virus in this region.

Methods: Specimens were collected in 24 forested localities at ground level with hand nets and mouth aspirators. Collections were made quarterly between October 2006 and July 2008 during daylight hours. Blood-meal identification was carried out in mosquitoes of the tribes Aedini, Mansoniini and Sabethini. The biotin/avidin sandwich ELISA was employed to determine six source types: bird, bovine, equine, rat, human and monkey.

Results: A total of 24,879 females of the three tribes were obtained, 245 (0.98%) of which were engorged. The presence of three different blood sources per engorged female was the predominant situation, and included 35.10% of the total of samples processed. Samples with two or four different sources were represented by 25.31% and 25.71%, of the specimens, respectively, while just 9.39% had only one type and 1.22%, five different sources. Aedes scapularis, Ae. serratus (Group), Psorophora albigenu and Ps. ferox were the most abundant species and accounted for about 95% of the engorged specimens. Of the principal vector species, Haemagogus janthinomys/capricornii was found with bird, bovine and primate blood. These sources were predominant and alternated top ranking as the most frequent source according to the mosquito species and collection site. In general, primate blood was the most prevalent source.

Conclusions: The human population of the region visits this ecotone frequently, which indicates the need for the periodical assessment of vaccination coverage against yellow fever. The frequency of non-human primate blood source in mosquito species that show minor vector importance in yellow fever virus transmission deserves attention. The eclectic feeding habits and some aspects of the interactions between potential vectors and reservoirs of yellow fever may be associated with the habitat fragmentation characteristic of the region. We recommend that further studies on the capacity and vector competence be performed on secondary vectors in extra-Amazonian region.

No MeSH data available.


Related in: MedlinePlus

Percentages of blood-source types for most abundant species of the total processed, by species. H: human (exclusive), NH: non-human primate (exclusive), P: primate (human + non-human), B: bird, C: cow, Ho: horse, R: rat and NR: not reagent. Aedes serratus (G): group including Ae. serratus and Ae. serratus/aenigmaticus.
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Fig2: Percentages of blood-source types for most abundant species of the total processed, by species. H: human (exclusive), NH: non-human primate (exclusive), P: primate (human + non-human), B: bird, C: cow, Ho: horse, R: rat and NR: not reagent. Aedes serratus (G): group including Ae. serratus and Ae. serratus/aenigmaticus.

Mentions: Among the most abundant species, Ps. albigenu presented a larger number of multiple sources, proportionally, than did Ae. scapularis, the majority being of four different origins. The difference between primates and NHP was also greater in Ps. albigenu than in Ae. scapularis, with a greater proportion of co-occurrence of human and monkey for the first specie. Bird blood occurred with greater frequency in Ps. albigenu and Ae. serratus; bovine blood was proportionally most frequent in Ps. ferox and Ae. serratus. Psorophora ferox was also notable for presenting the greatest frequency of NHP blood and the lowest frequency of human blood among the more abundant species (Figure 2). The only specimen of Hg. janthinomys/capricornii presented primate, bird and bovine blood, the most frequent combination among all the samples.Figure 2


Feeding habits of mosquitoes (Diptera: Culicidae) in an area of sylvatic transmission of yellow fever in the state of São Paulo, Brazil.

Mucci LF, Júnior RP, de Paula MB, Scandar SA, Pacchioni ML, Fernandes A, Consales CA - J Venom Anim Toxins Incl Trop Dis (2015)

Percentages of blood-source types for most abundant species of the total processed, by species. H: human (exclusive), NH: non-human primate (exclusive), P: primate (human + non-human), B: bird, C: cow, Ho: horse, R: rat and NR: not reagent. Aedes serratus (G): group including Ae. serratus and Ae. serratus/aenigmaticus.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4373060&req=5

Fig2: Percentages of blood-source types for most abundant species of the total processed, by species. H: human (exclusive), NH: non-human primate (exclusive), P: primate (human + non-human), B: bird, C: cow, Ho: horse, R: rat and NR: not reagent. Aedes serratus (G): group including Ae. serratus and Ae. serratus/aenigmaticus.
Mentions: Among the most abundant species, Ps. albigenu presented a larger number of multiple sources, proportionally, than did Ae. scapularis, the majority being of four different origins. The difference between primates and NHP was also greater in Ps. albigenu than in Ae. scapularis, with a greater proportion of co-occurrence of human and monkey for the first specie. Bird blood occurred with greater frequency in Ps. albigenu and Ae. serratus; bovine blood was proportionally most frequent in Ps. ferox and Ae. serratus. Psorophora ferox was also notable for presenting the greatest frequency of NHP blood and the lowest frequency of human blood among the more abundant species (Figure 2). The only specimen of Hg. janthinomys/capricornii presented primate, bird and bovine blood, the most frequent combination among all the samples.Figure 2

Bottom Line: Of the principal vector species, Haemagogus janthinomys/capricornii was found with bird, bovine and primate blood.The eclectic feeding habits and some aspects of the interactions between potential vectors and reservoirs of yellow fever may be associated with the habitat fragmentation characteristic of the region.We recommend that further studies on the capacity and vector competence be performed on secondary vectors in extra-Amazonian region.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biology and Ecology of Mosquitoes, Superintendence for Endemic Disease Control, State Health Secretariat, Taubaté, São Paulo State Brazil.

ABSTRACT

Background: The reintroduction of sylvatic yellow fever in the state of São Paulo after about six decades was confirmed in the Northwestern region in 2000, where in 2008 there also occurred an important epizootic. The purpose of this study was to investigate the feeding habits of culicids potentially involved in the sylvatic transmission of the virus in this region.

Methods: Specimens were collected in 24 forested localities at ground level with hand nets and mouth aspirators. Collections were made quarterly between October 2006 and July 2008 during daylight hours. Blood-meal identification was carried out in mosquitoes of the tribes Aedini, Mansoniini and Sabethini. The biotin/avidin sandwich ELISA was employed to determine six source types: bird, bovine, equine, rat, human and monkey.

Results: A total of 24,879 females of the three tribes were obtained, 245 (0.98%) of which were engorged. The presence of three different blood sources per engorged female was the predominant situation, and included 35.10% of the total of samples processed. Samples with two or four different sources were represented by 25.31% and 25.71%, of the specimens, respectively, while just 9.39% had only one type and 1.22%, five different sources. Aedes scapularis, Ae. serratus (Group), Psorophora albigenu and Ps. ferox were the most abundant species and accounted for about 95% of the engorged specimens. Of the principal vector species, Haemagogus janthinomys/capricornii was found with bird, bovine and primate blood. These sources were predominant and alternated top ranking as the most frequent source according to the mosquito species and collection site. In general, primate blood was the most prevalent source.

Conclusions: The human population of the region visits this ecotone frequently, which indicates the need for the periodical assessment of vaccination coverage against yellow fever. The frequency of non-human primate blood source in mosquito species that show minor vector importance in yellow fever virus transmission deserves attention. The eclectic feeding habits and some aspects of the interactions between potential vectors and reservoirs of yellow fever may be associated with the habitat fragmentation characteristic of the region. We recommend that further studies on the capacity and vector competence be performed on secondary vectors in extra-Amazonian region.

No MeSH data available.


Related in: MedlinePlus