Limits...
Rational design of a chalcogenopyrylium-based surface-enhanced resonance Raman scattering nanoprobe with attomolar sensitivity.

Harmsen S, Bedics MA, Wall MA, Huang R, Detty MR, Kircher MF - Nat Commun (2015)

Bottom Line: Raman imaging has surfaced as a promising optical modality that offers both.Here we report the design and synthesis of a group of near-infrared absorbing 2-thienyl-substituted chalcogenopyrylium dyes tailored to have high affinity for gold.When adsorbed onto gold nanoparticles, these dyes produce biocompatible SERRS nanoprobes with attomolar limits of detection amenable to ultrasensitive in vivo multiplexed tumour and disease marker detection.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, New York 10065, USA.

ABSTRACT
High sensitivity and specificity are two desirable features in biomedical imaging. Raman imaging has surfaced as a promising optical modality that offers both. Here we report the design and synthesis of a group of near-infrared absorbing 2-thienyl-substituted chalcogenopyrylium dyes tailored to have high affinity for gold. When adsorbed onto gold nanoparticles, these dyes produce biocompatible SERRS nanoprobes with attomolar limits of detection amenable to ultrasensitive in vivo multiplexed tumour and disease marker detection.

No MeSH data available.


Related in: MedlinePlus

Comparison of the SERRS-signal intensity of the optimized CP-dye 3 versus a widely used resonant dye IR792(A) Structure of the resonant dye IR792 and chalcogenopyrylium dye 3. (B) SERRS intensity of an equimolar amount of an IR792-based SERRS-nanoprobe and a 3-based SERRS-nanoprobe that were synthesized of an equimolar amount of the dyes. (C) Limits of detection of the IR792- (cyan) and 3- (red) based SERRS-nanoprobes were performed in triplicate and determined to be 1.0 fM and 100 attomolar, respectively (See also Supplementary Figure 4).
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Figure 4: Comparison of the SERRS-signal intensity of the optimized CP-dye 3 versus a widely used resonant dye IR792(A) Structure of the resonant dye IR792 and chalcogenopyrylium dye 3. (B) SERRS intensity of an equimolar amount of an IR792-based SERRS-nanoprobe and a 3-based SERRS-nanoprobe that were synthesized of an equimolar amount of the dyes. (C) Limits of detection of the IR792- (cyan) and 3- (red) based SERRS-nanoprobes were performed in triplicate and determined to be 1.0 fM and 100 attomolar, respectively (See also Supplementary Figure 4).

Mentions: In order to assess the quality of our optimized nanoprobe, thiopyrylium dye 3 and commercially available IR792 (Figure 4A), which has been previously used to generate surface-enhanced resonance Raman scattering nanoprobes,35 were studied. A direct comparison of the nanoprobes synthesized in the presence of equimolar (1.0 μM) amounts of 3 and IR792 shows a 5–6-fold higher signal for nanoprobes generated with dye 3 (Figure 4B). It is interesting to note that a fluorescence background is minimal in the SERRS spectra of the CP- and cyanine-based SERRS-nanoprobes (Supplementary Figure 3). Whereas fluorescence interference would not be expected from chalcogenopyrylium dyes containing heavy chalcogens that enhance intersystem crossing,36 fluorescence interference could be expected for the cyanine dye IR792. In fact, when equimolar amounts of the CP dyes 1–3 and IR792 were incorporated in silica (without gold nanoparticle), IR792 demonstrated strong fluorescence when excited at 785 nm (50 μW, 1.0 s acquisition time), while minimal fluorescence was observed for CP 1–3. As shown in Figure 4B and Supplementary Figure 3, the fluorescence interference of the cyanine dye IR792 is minimal in its SERRS spectrum. This is due to quenching effects near the surface of the nanoparticle.37


Rational design of a chalcogenopyrylium-based surface-enhanced resonance Raman scattering nanoprobe with attomolar sensitivity.

Harmsen S, Bedics MA, Wall MA, Huang R, Detty MR, Kircher MF - Nat Commun (2015)

Comparison of the SERRS-signal intensity of the optimized CP-dye 3 versus a widely used resonant dye IR792(A) Structure of the resonant dye IR792 and chalcogenopyrylium dye 3. (B) SERRS intensity of an equimolar amount of an IR792-based SERRS-nanoprobe and a 3-based SERRS-nanoprobe that were synthesized of an equimolar amount of the dyes. (C) Limits of detection of the IR792- (cyan) and 3- (red) based SERRS-nanoprobes were performed in triplicate and determined to be 1.0 fM and 100 attomolar, respectively (See also Supplementary Figure 4).
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Related In: Results  -  Collection

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Figure 4: Comparison of the SERRS-signal intensity of the optimized CP-dye 3 versus a widely used resonant dye IR792(A) Structure of the resonant dye IR792 and chalcogenopyrylium dye 3. (B) SERRS intensity of an equimolar amount of an IR792-based SERRS-nanoprobe and a 3-based SERRS-nanoprobe that were synthesized of an equimolar amount of the dyes. (C) Limits of detection of the IR792- (cyan) and 3- (red) based SERRS-nanoprobes were performed in triplicate and determined to be 1.0 fM and 100 attomolar, respectively (See also Supplementary Figure 4).
Mentions: In order to assess the quality of our optimized nanoprobe, thiopyrylium dye 3 and commercially available IR792 (Figure 4A), which has been previously used to generate surface-enhanced resonance Raman scattering nanoprobes,35 were studied. A direct comparison of the nanoprobes synthesized in the presence of equimolar (1.0 μM) amounts of 3 and IR792 shows a 5–6-fold higher signal for nanoprobes generated with dye 3 (Figure 4B). It is interesting to note that a fluorescence background is minimal in the SERRS spectra of the CP- and cyanine-based SERRS-nanoprobes (Supplementary Figure 3). Whereas fluorescence interference would not be expected from chalcogenopyrylium dyes containing heavy chalcogens that enhance intersystem crossing,36 fluorescence interference could be expected for the cyanine dye IR792. In fact, when equimolar amounts of the CP dyes 1–3 and IR792 were incorporated in silica (without gold nanoparticle), IR792 demonstrated strong fluorescence when excited at 785 nm (50 μW, 1.0 s acquisition time), while minimal fluorescence was observed for CP 1–3. As shown in Figure 4B and Supplementary Figure 3, the fluorescence interference of the cyanine dye IR792 is minimal in its SERRS spectrum. This is due to quenching effects near the surface of the nanoparticle.37

Bottom Line: Raman imaging has surfaced as a promising optical modality that offers both.Here we report the design and synthesis of a group of near-infrared absorbing 2-thienyl-substituted chalcogenopyrylium dyes tailored to have high affinity for gold.When adsorbed onto gold nanoparticles, these dyes produce biocompatible SERRS nanoprobes with attomolar limits of detection amenable to ultrasensitive in vivo multiplexed tumour and disease marker detection.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, New York 10065, USA.

ABSTRACT
High sensitivity and specificity are two desirable features in biomedical imaging. Raman imaging has surfaced as a promising optical modality that offers both. Here we report the design and synthesis of a group of near-infrared absorbing 2-thienyl-substituted chalcogenopyrylium dyes tailored to have high affinity for gold. When adsorbed onto gold nanoparticles, these dyes produce biocompatible SERRS nanoprobes with attomolar limits of detection amenable to ultrasensitive in vivo multiplexed tumour and disease marker detection.

No MeSH data available.


Related in: MedlinePlus