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The impact of diclofenac and ibuprofen on biofilm formation on the surface of polypropylene mesh.

Reśliński A, Dąbrowiecki S, Głowacka K - Hernia (2013)

Bottom Line: The assessment of the impact of non-steroidal anti-inflammatory drugs (NSAIDs) on biofilm formation was carried out with the use of qualitative method (TTC reduction), quantitative (tenfold serial dilution) and with the use of scanning electron microscope (SEM).In the examination with the use of SEM it was stated that the effect of isolates incubation in the medium with NSAIDs was decrease of the number of bacteria adjacent to the biomaterial surface.Diclofenac and ibuprofen in the concentration obtained in the serum limit the formation of biofilm by S. aureus and E. coli.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery and Transplantology, Ludwik Rydygier College of Medicine in Bydgoszcz, Nicolaus Copernicus University in Toruń, M. Skłodowskiej-Curie 9 Str., 85-094, Bydgoszcz, Poland, bigar@wp.pl.

ABSTRACT

Purpose: The difficulties related to treatment of deep surgical site infection involve formation of biofilm on the surface of synthetic material. It is considered that in treatment of infections involving formation of biofilm, concomitant therapy shall be applied covering anti-inflammatory drugs. The purpose of the work was to assess the impact of diclofenac and ibuprofen on bacterial biofilm formation on the surface of monofilament polypropylene mesh.

Materials and methods: The study involved 70 strains of Staphylococcus aureus and 70 strains of Escherichia coli isolated from different patients and those which differ with chromosomal DNA pattern within the species. The assessment of the impact of non-steroidal anti-inflammatory drugs (NSAIDs) on biofilm formation was carried out with the use of qualitative method (TTC reduction), quantitative (tenfold serial dilution) and with the use of scanning electron microscope (SEM).

Results: In the qualitative assessment, after incubation in the medium containing NSAIDs statistically significant growth of S. aureus strain amount and E. coli which poorly make up biofilm was stated. Quantitative examination indicated characteristic decrease of the number of colony forming units in 1 ml of the suspension isolated from bacterial biofilm formed as a result of incubation of isolates in the medium with the addition of examined NSAIDs in comparison to biofilm from control regimen. In the examination with the use of SEM it was stated that the effect of isolates incubation in the medium with NSAIDs was decrease of the number of bacteria adjacent to the biomaterial surface.

Conclusions: Diclofenac and ibuprofen in the concentration obtained in the serum limit the formation of biofilm by S. aureus and E. coli.

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Related in: MedlinePlus

Decrease of E. coli adhesion to surface of monofilament polypropylene mesh after incubation in the medium with ibuprofen; SEM—resolution of ×1,500
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Fig3: Decrease of E. coli adhesion to surface of monofilament polypropylene mesh after incubation in the medium with ibuprofen; SEM—resolution of ×1,500

Mentions: The effect of incubation of strains of S. aureus and E coli in mediums with the addition of examined NSAIDs was the decrease of the number of bacteria adjacent to the surface of monofilament polypropylene mesh (Figs. 2, 3) in relation to control regimen (Figs. 4, 5). Single bacteria clusters were observed (Figs. 6, 7).Fig. 2


The impact of diclofenac and ibuprofen on biofilm formation on the surface of polypropylene mesh.

Reśliński A, Dąbrowiecki S, Głowacka K - Hernia (2013)

Decrease of E. coli adhesion to surface of monofilament polypropylene mesh after incubation in the medium with ibuprofen; SEM—resolution of ×1,500
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4372680&req=5

Fig3: Decrease of E. coli adhesion to surface of monofilament polypropylene mesh after incubation in the medium with ibuprofen; SEM—resolution of ×1,500
Mentions: The effect of incubation of strains of S. aureus and E coli in mediums with the addition of examined NSAIDs was the decrease of the number of bacteria adjacent to the surface of monofilament polypropylene mesh (Figs. 2, 3) in relation to control regimen (Figs. 4, 5). Single bacteria clusters were observed (Figs. 6, 7).Fig. 2

Bottom Line: The assessment of the impact of non-steroidal anti-inflammatory drugs (NSAIDs) on biofilm formation was carried out with the use of qualitative method (TTC reduction), quantitative (tenfold serial dilution) and with the use of scanning electron microscope (SEM).In the examination with the use of SEM it was stated that the effect of isolates incubation in the medium with NSAIDs was decrease of the number of bacteria adjacent to the biomaterial surface.Diclofenac and ibuprofen in the concentration obtained in the serum limit the formation of biofilm by S. aureus and E. coli.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery and Transplantology, Ludwik Rydygier College of Medicine in Bydgoszcz, Nicolaus Copernicus University in Toruń, M. Skłodowskiej-Curie 9 Str., 85-094, Bydgoszcz, Poland, bigar@wp.pl.

ABSTRACT

Purpose: The difficulties related to treatment of deep surgical site infection involve formation of biofilm on the surface of synthetic material. It is considered that in treatment of infections involving formation of biofilm, concomitant therapy shall be applied covering anti-inflammatory drugs. The purpose of the work was to assess the impact of diclofenac and ibuprofen on bacterial biofilm formation on the surface of monofilament polypropylene mesh.

Materials and methods: The study involved 70 strains of Staphylococcus aureus and 70 strains of Escherichia coli isolated from different patients and those which differ with chromosomal DNA pattern within the species. The assessment of the impact of non-steroidal anti-inflammatory drugs (NSAIDs) on biofilm formation was carried out with the use of qualitative method (TTC reduction), quantitative (tenfold serial dilution) and with the use of scanning electron microscope (SEM).

Results: In the qualitative assessment, after incubation in the medium containing NSAIDs statistically significant growth of S. aureus strain amount and E. coli which poorly make up biofilm was stated. Quantitative examination indicated characteristic decrease of the number of colony forming units in 1 ml of the suspension isolated from bacterial biofilm formed as a result of incubation of isolates in the medium with the addition of examined NSAIDs in comparison to biofilm from control regimen. In the examination with the use of SEM it was stated that the effect of isolates incubation in the medium with NSAIDs was decrease of the number of bacteria adjacent to the biomaterial surface.

Conclusions: Diclofenac and ibuprofen in the concentration obtained in the serum limit the formation of biofilm by S. aureus and E. coli.

Show MeSH
Related in: MedlinePlus