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The impact of diclofenac and ibuprofen on biofilm formation on the surface of polypropylene mesh.

Reśliński A, Dąbrowiecki S, Głowacka K - Hernia (2013)

Bottom Line: The assessment of the impact of non-steroidal anti-inflammatory drugs (NSAIDs) on biofilm formation was carried out with the use of qualitative method (TTC reduction), quantitative (tenfold serial dilution) and with the use of scanning electron microscope (SEM).In the examination with the use of SEM it was stated that the effect of isolates incubation in the medium with NSAIDs was decrease of the number of bacteria adjacent to the biomaterial surface.Diclofenac and ibuprofen in the concentration obtained in the serum limit the formation of biofilm by S. aureus and E. coli.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery and Transplantology, Ludwik Rydygier College of Medicine in Bydgoszcz, Nicolaus Copernicus University in Toruń, M. Skłodowskiej-Curie 9 Str., 85-094, Bydgoszcz, Poland, bigar@wp.pl.

ABSTRACT

Purpose: The difficulties related to treatment of deep surgical site infection involve formation of biofilm on the surface of synthetic material. It is considered that in treatment of infections involving formation of biofilm, concomitant therapy shall be applied covering anti-inflammatory drugs. The purpose of the work was to assess the impact of diclofenac and ibuprofen on bacterial biofilm formation on the surface of monofilament polypropylene mesh.

Materials and methods: The study involved 70 strains of Staphylococcus aureus and 70 strains of Escherichia coli isolated from different patients and those which differ with chromosomal DNA pattern within the species. The assessment of the impact of non-steroidal anti-inflammatory drugs (NSAIDs) on biofilm formation was carried out with the use of qualitative method (TTC reduction), quantitative (tenfold serial dilution) and with the use of scanning electron microscope (SEM).

Results: In the qualitative assessment, after incubation in the medium containing NSAIDs statistically significant growth of S. aureus strain amount and E. coli which poorly make up biofilm was stated. Quantitative examination indicated characteristic decrease of the number of colony forming units in 1 ml of the suspension isolated from bacterial biofilm formed as a result of incubation of isolates in the medium with the addition of examined NSAIDs in comparison to biofilm from control regimen. In the examination with the use of SEM it was stated that the effect of isolates incubation in the medium with NSAIDs was decrease of the number of bacteria adjacent to the biomaterial surface.

Conclusions: Diclofenac and ibuprofen in the concentration obtained in the serum limit the formation of biofilm by S. aureus and E. coli.

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Related in: MedlinePlus

Evaluation of biofilm formation using qualitative method (explanation in text)
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Fig1: Evaluation of biofilm formation using qualitative method (explanation in text)

Mentions: In the examination applying qualitative method [15, 16] the change of color of the mesh surface that occurs as a result of reduction of colorless 2,3,5-triphenyl tetrazolium chloride (TTC) to red formazan carried out by metabolically active microbes was evaluated. After 72-h incubation, implant fragments were washed with 0.9 % phosphate buffered saline solution (PBS, BTL, Łódź, Poland) with pH 7.2 and was placed in 4 ml of sterile TSB medium with the addition of 20 μl of 1 % 2,3,5-triphenyltetrazolium chloride solution (TTC, POCH S.A., Gliwice, Poland). The samples were incubated for 24 h at the temperature of 37 °C in aerobic atmosphere and then biofilm formation was assessed according to assumed scale: 0—no biofilm formation (or fragment of sterile mesh), 1—poor biofilm formation (slight pink dotting of the mesh), 2—strong biofilm formation (solid pink coloring of the entire mesh), 3—very strong biofilm formation (red coloring of the mesh) (Fig. 1). The examination for each strain was carried out in threefold repetition.Fig. 1


The impact of diclofenac and ibuprofen on biofilm formation on the surface of polypropylene mesh.

Reśliński A, Dąbrowiecki S, Głowacka K - Hernia (2013)

Evaluation of biofilm formation using qualitative method (explanation in text)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4372680&req=5

Fig1: Evaluation of biofilm formation using qualitative method (explanation in text)
Mentions: In the examination applying qualitative method [15, 16] the change of color of the mesh surface that occurs as a result of reduction of colorless 2,3,5-triphenyl tetrazolium chloride (TTC) to red formazan carried out by metabolically active microbes was evaluated. After 72-h incubation, implant fragments were washed with 0.9 % phosphate buffered saline solution (PBS, BTL, Łódź, Poland) with pH 7.2 and was placed in 4 ml of sterile TSB medium with the addition of 20 μl of 1 % 2,3,5-triphenyltetrazolium chloride solution (TTC, POCH S.A., Gliwice, Poland). The samples were incubated for 24 h at the temperature of 37 °C in aerobic atmosphere and then biofilm formation was assessed according to assumed scale: 0—no biofilm formation (or fragment of sterile mesh), 1—poor biofilm formation (slight pink dotting of the mesh), 2—strong biofilm formation (solid pink coloring of the entire mesh), 3—very strong biofilm formation (red coloring of the mesh) (Fig. 1). The examination for each strain was carried out in threefold repetition.Fig. 1

Bottom Line: The assessment of the impact of non-steroidal anti-inflammatory drugs (NSAIDs) on biofilm formation was carried out with the use of qualitative method (TTC reduction), quantitative (tenfold serial dilution) and with the use of scanning electron microscope (SEM).In the examination with the use of SEM it was stated that the effect of isolates incubation in the medium with NSAIDs was decrease of the number of bacteria adjacent to the biomaterial surface.Diclofenac and ibuprofen in the concentration obtained in the serum limit the formation of biofilm by S. aureus and E. coli.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery and Transplantology, Ludwik Rydygier College of Medicine in Bydgoszcz, Nicolaus Copernicus University in Toruń, M. Skłodowskiej-Curie 9 Str., 85-094, Bydgoszcz, Poland, bigar@wp.pl.

ABSTRACT

Purpose: The difficulties related to treatment of deep surgical site infection involve formation of biofilm on the surface of synthetic material. It is considered that in treatment of infections involving formation of biofilm, concomitant therapy shall be applied covering anti-inflammatory drugs. The purpose of the work was to assess the impact of diclofenac and ibuprofen on bacterial biofilm formation on the surface of monofilament polypropylene mesh.

Materials and methods: The study involved 70 strains of Staphylococcus aureus and 70 strains of Escherichia coli isolated from different patients and those which differ with chromosomal DNA pattern within the species. The assessment of the impact of non-steroidal anti-inflammatory drugs (NSAIDs) on biofilm formation was carried out with the use of qualitative method (TTC reduction), quantitative (tenfold serial dilution) and with the use of scanning electron microscope (SEM).

Results: In the qualitative assessment, after incubation in the medium containing NSAIDs statistically significant growth of S. aureus strain amount and E. coli which poorly make up biofilm was stated. Quantitative examination indicated characteristic decrease of the number of colony forming units in 1 ml of the suspension isolated from bacterial biofilm formed as a result of incubation of isolates in the medium with the addition of examined NSAIDs in comparison to biofilm from control regimen. In the examination with the use of SEM it was stated that the effect of isolates incubation in the medium with NSAIDs was decrease of the number of bacteria adjacent to the biomaterial surface.

Conclusions: Diclofenac and ibuprofen in the concentration obtained in the serum limit the formation of biofilm by S. aureus and E. coli.

Show MeSH
Related in: MedlinePlus