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Cell type-dependent changes in CdSe/ZnS quantum dot uptake and toxic endpoints.

Manshian BB, Soenen SJ, Al-Ali A, Brown A, Hondow N, Wills J, Jenkins GJ, Doak SH - Toxicol. Sci. (2015)

Bottom Line: Following thorough physicochemical characterization, cellular uptake, cytotoxicity, and gross chromosomal damage were measured.BEAS-2B cells demonstrated the highest level of QDs uptake yet displayed a strong resilience with minimal genotoxicity following exposure to these NPs.Thus, this study demonstrates that in addition to nanomaterial physicochemical characterization, a clear understanding of cell type-dependent variation in uptake coupled to the inherently different capacities of the cell types to cope with exposure to these exogenous materials are all required to predict genotoxicity.

View Article: PubMed Central - PubMed

Affiliation: *Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK, Department of Medicine, Biomedical NMR Unit-MoSAIC, KU Leuven, B-3000 Leuven, Belgium and Institute for Materials Research, SCaPE, University of Leeds, Leeds LS2 9JT, UK *Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK, Department of Medicine, Biomedical NMR Unit-MoSAIC, KU Leuven, B-3000 Leuven, Belgium and Institute for Materials Research, SCaPE, University of Leeds, Leeds LS2 9JT, UK s.h.doak@swansea.ac.uk.

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ROS induction in TK6 and HFF-1 cells treated with: (A, C) amine-, and (B, D) carboxyl-QDs for 4 or 24 h in full (dark gray) and reduced (light gray) serum conditions. Data are expressed as fluorescence intensity levels relative to untreated control cells and are represented as the mean ± standard error of the mean. The relative fluorescence intensity for the H2O2 positive control was 250 ± 50% and 200 ± 45% for the TK6 and HFF-1 exposed cells, respectively. Where appropriate, the degree of significance is indicated (*P < 0.05, **P < 0.01, ***P < 0.001).
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kfv002-F7: ROS induction in TK6 and HFF-1 cells treated with: (A, C) amine-, and (B, D) carboxyl-QDs for 4 or 24 h in full (dark gray) and reduced (light gray) serum conditions. Data are expressed as fluorescence intensity levels relative to untreated control cells and are represented as the mean ± standard error of the mean. The relative fluorescence intensity for the H2O2 positive control was 250 ± 50% and 200 ± 45% for the TK6 and HFF-1 exposed cells, respectively. Where appropriate, the degree of significance is indicated (*P < 0.05, **P < 0.01, ***P < 0.001).

Mentions: The production of ROS was investigated due to its association with toxicity following exposure to certain NPs. However, only minimal cytoplasmic ROS was detected, mainly in HFF-1 cells exposed to the carboxyl-QD and TK6 cells exposed to amine-QDs (Fig. 7). With respect to mitochondrial membrane permeability, no observable effects were seen in HFF-1 or TK6 cells exposed to any QDs in full serum conditions (Fig. 8). In contrast, a significant and concentration-dependent increase in MMP was recorded in TK6 and HFF-1 cells treated with carboxyl-QDs in reduced serum containing media. This increase in MMP occurred after 4 and 24 h treatments in TK6 and HFF-1 cells, respectively (Fig. 8B and 8D).FIG. 7.


Cell type-dependent changes in CdSe/ZnS quantum dot uptake and toxic endpoints.

Manshian BB, Soenen SJ, Al-Ali A, Brown A, Hondow N, Wills J, Jenkins GJ, Doak SH - Toxicol. Sci. (2015)

ROS induction in TK6 and HFF-1 cells treated with: (A, C) amine-, and (B, D) carboxyl-QDs for 4 or 24 h in full (dark gray) and reduced (light gray) serum conditions. Data are expressed as fluorescence intensity levels relative to untreated control cells and are represented as the mean ± standard error of the mean. The relative fluorescence intensity for the H2O2 positive control was 250 ± 50% and 200 ± 45% for the TK6 and HFF-1 exposed cells, respectively. Where appropriate, the degree of significance is indicated (*P < 0.05, **P < 0.01, ***P < 0.001).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4372665&req=5

kfv002-F7: ROS induction in TK6 and HFF-1 cells treated with: (A, C) amine-, and (B, D) carboxyl-QDs for 4 or 24 h in full (dark gray) and reduced (light gray) serum conditions. Data are expressed as fluorescence intensity levels relative to untreated control cells and are represented as the mean ± standard error of the mean. The relative fluorescence intensity for the H2O2 positive control was 250 ± 50% and 200 ± 45% for the TK6 and HFF-1 exposed cells, respectively. Where appropriate, the degree of significance is indicated (*P < 0.05, **P < 0.01, ***P < 0.001).
Mentions: The production of ROS was investigated due to its association with toxicity following exposure to certain NPs. However, only minimal cytoplasmic ROS was detected, mainly in HFF-1 cells exposed to the carboxyl-QD and TK6 cells exposed to amine-QDs (Fig. 7). With respect to mitochondrial membrane permeability, no observable effects were seen in HFF-1 or TK6 cells exposed to any QDs in full serum conditions (Fig. 8). In contrast, a significant and concentration-dependent increase in MMP was recorded in TK6 and HFF-1 cells treated with carboxyl-QDs in reduced serum containing media. This increase in MMP occurred after 4 and 24 h treatments in TK6 and HFF-1 cells, respectively (Fig. 8B and 8D).FIG. 7.

Bottom Line: Following thorough physicochemical characterization, cellular uptake, cytotoxicity, and gross chromosomal damage were measured.BEAS-2B cells demonstrated the highest level of QDs uptake yet displayed a strong resilience with minimal genotoxicity following exposure to these NPs.Thus, this study demonstrates that in addition to nanomaterial physicochemical characterization, a clear understanding of cell type-dependent variation in uptake coupled to the inherently different capacities of the cell types to cope with exposure to these exogenous materials are all required to predict genotoxicity.

View Article: PubMed Central - PubMed

Affiliation: *Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK, Department of Medicine, Biomedical NMR Unit-MoSAIC, KU Leuven, B-3000 Leuven, Belgium and Institute for Materials Research, SCaPE, University of Leeds, Leeds LS2 9JT, UK *Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK, Department of Medicine, Biomedical NMR Unit-MoSAIC, KU Leuven, B-3000 Leuven, Belgium and Institute for Materials Research, SCaPE, University of Leeds, Leeds LS2 9JT, UK s.h.doak@swansea.ac.uk.

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Related in: MedlinePlus