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Cell type-dependent changes in CdSe/ZnS quantum dot uptake and toxic endpoints.

Manshian BB, Soenen SJ, Al-Ali A, Brown A, Hondow N, Wills J, Jenkins GJ, Doak SH - Toxicol. Sci. (2015)

Bottom Line: Following thorough physicochemical characterization, cellular uptake, cytotoxicity, and gross chromosomal damage were measured.BEAS-2B cells demonstrated the highest level of QDs uptake yet displayed a strong resilience with minimal genotoxicity following exposure to these NPs.Thus, this study demonstrates that in addition to nanomaterial physicochemical characterization, a clear understanding of cell type-dependent variation in uptake coupled to the inherently different capacities of the cell types to cope with exposure to these exogenous materials are all required to predict genotoxicity.

View Article: PubMed Central - PubMed

Affiliation: *Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK, Department of Medicine, Biomedical NMR Unit-MoSAIC, KU Leuven, B-3000 Leuven, Belgium and Institute for Materials Research, SCaPE, University of Leeds, Leeds LS2 9JT, UK *Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK, Department of Medicine, Biomedical NMR Unit-MoSAIC, KU Leuven, B-3000 Leuven, Belgium and Institute for Materials Research, SCaPE, University of Leeds, Leeds LS2 9JT, UK s.h.doak@swansea.ac.uk.

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Ratio of micronuclei (MN) containing whole chromosomes (centromere positive) to DNA fragments (centromere negative) for TK6 cells exposed to QDs. Pancentromeric staining in TK6 and HFF-1 cells exposed to amine- and carboxyl-QDs for 24 h in (A) TK6 cells 1% serum; (B) TK6 cells 10% serum; and (C) HFF-1 cells 15% serum containing medium. (D, E) Representative fluorescence images of a binucleated TK6 cell with (D) or without (E) a centromere positive micronucleus (indicated by white arrows). Centromere-positive and centromere-negative MN were differentiated by the presence of bright yellow-green signal after pancentromeric antibody staining. Nuclei were counterstained with DAPI.
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kfv002-F6: Ratio of micronuclei (MN) containing whole chromosomes (centromere positive) to DNA fragments (centromere negative) for TK6 cells exposed to QDs. Pancentromeric staining in TK6 and HFF-1 cells exposed to amine- and carboxyl-QDs for 24 h in (A) TK6 cells 1% serum; (B) TK6 cells 10% serum; and (C) HFF-1 cells 15% serum containing medium. (D, E) Representative fluorescence images of a binucleated TK6 cell with (D) or without (E) a centromere positive micronucleus (indicated by white arrows). Centromere-positive and centromere-negative MN were differentiated by the presence of bright yellow-green signal after pancentromeric antibody staining. Nuclei were counterstained with DAPI.

Mentions: Pancentromeric staining was utilized to determine whether the gross chromosomal damage induced by the QDs was caused by clastogenic or aneugenic events. These experiments were conducted in both TK6 and HFF-1 cells in both full and reduced serum containing media. In TK6 cells amine-QDs showed a concentration-dependent trend of increasing aneuploidy (ranging from 50% to 76% MN containing whole chromosomes) induced in both 1% and 10% serum containing media (Fig. 6). This effect was less pronounced with the carboxyl-QDs. In HFF-1 cells pancentromeric detection was only performed in full serum conditions due to the absence of sufficient MN in the reduced serum conditions. Interestingly, in this cell line both QDs induced mainly clastogenic events (Fig. 6C).FIG. 6.


Cell type-dependent changes in CdSe/ZnS quantum dot uptake and toxic endpoints.

Manshian BB, Soenen SJ, Al-Ali A, Brown A, Hondow N, Wills J, Jenkins GJ, Doak SH - Toxicol. Sci. (2015)

Ratio of micronuclei (MN) containing whole chromosomes (centromere positive) to DNA fragments (centromere negative) for TK6 cells exposed to QDs. Pancentromeric staining in TK6 and HFF-1 cells exposed to amine- and carboxyl-QDs for 24 h in (A) TK6 cells 1% serum; (B) TK6 cells 10% serum; and (C) HFF-1 cells 15% serum containing medium. (D, E) Representative fluorescence images of a binucleated TK6 cell with (D) or without (E) a centromere positive micronucleus (indicated by white arrows). Centromere-positive and centromere-negative MN were differentiated by the presence of bright yellow-green signal after pancentromeric antibody staining. Nuclei were counterstained with DAPI.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4372665&req=5

kfv002-F6: Ratio of micronuclei (MN) containing whole chromosomes (centromere positive) to DNA fragments (centromere negative) for TK6 cells exposed to QDs. Pancentromeric staining in TK6 and HFF-1 cells exposed to amine- and carboxyl-QDs for 24 h in (A) TK6 cells 1% serum; (B) TK6 cells 10% serum; and (C) HFF-1 cells 15% serum containing medium. (D, E) Representative fluorescence images of a binucleated TK6 cell with (D) or without (E) a centromere positive micronucleus (indicated by white arrows). Centromere-positive and centromere-negative MN were differentiated by the presence of bright yellow-green signal after pancentromeric antibody staining. Nuclei were counterstained with DAPI.
Mentions: Pancentromeric staining was utilized to determine whether the gross chromosomal damage induced by the QDs was caused by clastogenic or aneugenic events. These experiments were conducted in both TK6 and HFF-1 cells in both full and reduced serum containing media. In TK6 cells amine-QDs showed a concentration-dependent trend of increasing aneuploidy (ranging from 50% to 76% MN containing whole chromosomes) induced in both 1% and 10% serum containing media (Fig. 6). This effect was less pronounced with the carboxyl-QDs. In HFF-1 cells pancentromeric detection was only performed in full serum conditions due to the absence of sufficient MN in the reduced serum conditions. Interestingly, in this cell line both QDs induced mainly clastogenic events (Fig. 6C).FIG. 6.

Bottom Line: Following thorough physicochemical characterization, cellular uptake, cytotoxicity, and gross chromosomal damage were measured.BEAS-2B cells demonstrated the highest level of QDs uptake yet displayed a strong resilience with minimal genotoxicity following exposure to these NPs.Thus, this study demonstrates that in addition to nanomaterial physicochemical characterization, a clear understanding of cell type-dependent variation in uptake coupled to the inherently different capacities of the cell types to cope with exposure to these exogenous materials are all required to predict genotoxicity.

View Article: PubMed Central - PubMed

Affiliation: *Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK, Department of Medicine, Biomedical NMR Unit-MoSAIC, KU Leuven, B-3000 Leuven, Belgium and Institute for Materials Research, SCaPE, University of Leeds, Leeds LS2 9JT, UK *Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK, Department of Medicine, Biomedical NMR Unit-MoSAIC, KU Leuven, B-3000 Leuven, Belgium and Institute for Materials Research, SCaPE, University of Leeds, Leeds LS2 9JT, UK s.h.doak@swansea.ac.uk.

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Related in: MedlinePlus