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Cell type-dependent changes in CdSe/ZnS quantum dot uptake and toxic endpoints.

Manshian BB, Soenen SJ, Al-Ali A, Brown A, Hondow N, Wills J, Jenkins GJ, Doak SH - Toxicol. Sci. (2015)

Bottom Line: Following thorough physicochemical characterization, cellular uptake, cytotoxicity, and gross chromosomal damage were measured.BEAS-2B cells demonstrated the highest level of QDs uptake yet displayed a strong resilience with minimal genotoxicity following exposure to these NPs.Thus, this study demonstrates that in addition to nanomaterial physicochemical characterization, a clear understanding of cell type-dependent variation in uptake coupled to the inherently different capacities of the cell types to cope with exposure to these exogenous materials are all required to predict genotoxicity.

View Article: PubMed Central - PubMed

Affiliation: *Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK, Department of Medicine, Biomedical NMR Unit-MoSAIC, KU Leuven, B-3000 Leuven, Belgium and Institute for Materials Research, SCaPE, University of Leeds, Leeds LS2 9JT, UK *Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK, Department of Medicine, Biomedical NMR Unit-MoSAIC, KU Leuven, B-3000 Leuven, Belgium and Institute for Materials Research, SCaPE, University of Leeds, Leeds LS2 9JT, UK s.h.doak@swansea.ac.uk.

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Cytotoxicity induced following exposure of human cells to QDs. (A, C, D) amine- and (B, D, E) carboxyl-QDs exposure to (A, B) HFF-1; (C, D) BEAS-2B; and (E, F) TK6 cells for 1 and 3 cell cycles exposure times in full and reduced serum containing media. Data are presented as mean ± SD.
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kfv002-F4: Cytotoxicity induced following exposure of human cells to QDs. (A, C, D) amine- and (B, D, E) carboxyl-QDs exposure to (A, B) HFF-1; (C, D) BEAS-2B; and (E, F) TK6 cells for 1 and 3 cell cycles exposure times in full and reduced serum containing media. Data are presented as mean ± SD.

Mentions: Results of the RPD analysis revealed that no significant cytotoxicity was observed in BEAS-2B cells exposed to carboxyl- or amine-QDs in the presence of 2% or 10% serum after 1 or 3 cell cycles (Figs. 4C and 4D). Exposing HFF-1 cells to carboxyl-QDs in full (15%) serum containing media for 1 cell cycle induced notable cytotoxicity, which increased following 3 cell cycle exposures with significantly decreased cell viability (down to ≤ 38.5%) at concentrations ≥ 7.5 nM. This was however not the case in reduced serum experiments where no toxicity was observed (Figs. 4A and 4B). TK6 and HFF-1 cells suffered high levels of toxicity at concentrations higher than 15 nM (data not shown on graph) while BEAS-2B cells were able to tolerate concentrations of up to 20 nM (Figs. 4C and 4D).FIG. 4.


Cell type-dependent changes in CdSe/ZnS quantum dot uptake and toxic endpoints.

Manshian BB, Soenen SJ, Al-Ali A, Brown A, Hondow N, Wills J, Jenkins GJ, Doak SH - Toxicol. Sci. (2015)

Cytotoxicity induced following exposure of human cells to QDs. (A, C, D) amine- and (B, D, E) carboxyl-QDs exposure to (A, B) HFF-1; (C, D) BEAS-2B; and (E, F) TK6 cells for 1 and 3 cell cycles exposure times in full and reduced serum containing media. Data are presented as mean ± SD.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4372665&req=5

kfv002-F4: Cytotoxicity induced following exposure of human cells to QDs. (A, C, D) amine- and (B, D, E) carboxyl-QDs exposure to (A, B) HFF-1; (C, D) BEAS-2B; and (E, F) TK6 cells for 1 and 3 cell cycles exposure times in full and reduced serum containing media. Data are presented as mean ± SD.
Mentions: Results of the RPD analysis revealed that no significant cytotoxicity was observed in BEAS-2B cells exposed to carboxyl- or amine-QDs in the presence of 2% or 10% serum after 1 or 3 cell cycles (Figs. 4C and 4D). Exposing HFF-1 cells to carboxyl-QDs in full (15%) serum containing media for 1 cell cycle induced notable cytotoxicity, which increased following 3 cell cycle exposures with significantly decreased cell viability (down to ≤ 38.5%) at concentrations ≥ 7.5 nM. This was however not the case in reduced serum experiments where no toxicity was observed (Figs. 4A and 4B). TK6 and HFF-1 cells suffered high levels of toxicity at concentrations higher than 15 nM (data not shown on graph) while BEAS-2B cells were able to tolerate concentrations of up to 20 nM (Figs. 4C and 4D).FIG. 4.

Bottom Line: Following thorough physicochemical characterization, cellular uptake, cytotoxicity, and gross chromosomal damage were measured.BEAS-2B cells demonstrated the highest level of QDs uptake yet displayed a strong resilience with minimal genotoxicity following exposure to these NPs.Thus, this study demonstrates that in addition to nanomaterial physicochemical characterization, a clear understanding of cell type-dependent variation in uptake coupled to the inherently different capacities of the cell types to cope with exposure to these exogenous materials are all required to predict genotoxicity.

View Article: PubMed Central - PubMed

Affiliation: *Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK, Department of Medicine, Biomedical NMR Unit-MoSAIC, KU Leuven, B-3000 Leuven, Belgium and Institute for Materials Research, SCaPE, University of Leeds, Leeds LS2 9JT, UK *Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK, Department of Medicine, Biomedical NMR Unit-MoSAIC, KU Leuven, B-3000 Leuven, Belgium and Institute for Materials Research, SCaPE, University of Leeds, Leeds LS2 9JT, UK s.h.doak@swansea.ac.uk.

Show MeSH
Related in: MedlinePlus