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Cell type-dependent changes in CdSe/ZnS quantum dot uptake and toxic endpoints.

Manshian BB, Soenen SJ, Al-Ali A, Brown A, Hondow N, Wills J, Jenkins GJ, Doak SH - Toxicol. Sci. (2015)

Bottom Line: Following thorough physicochemical characterization, cellular uptake, cytotoxicity, and gross chromosomal damage were measured.BEAS-2B cells demonstrated the highest level of QDs uptake yet displayed a strong resilience with minimal genotoxicity following exposure to these NPs.Thus, this study demonstrates that in addition to nanomaterial physicochemical characterization, a clear understanding of cell type-dependent variation in uptake coupled to the inherently different capacities of the cell types to cope with exposure to these exogenous materials are all required to predict genotoxicity.

View Article: PubMed Central - PubMed

Affiliation: *Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK, Department of Medicine, Biomedical NMR Unit-MoSAIC, KU Leuven, B-3000 Leuven, Belgium and Institute for Materials Research, SCaPE, University of Leeds, Leeds LS2 9JT, UK *Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK, Department of Medicine, Biomedical NMR Unit-MoSAIC, KU Leuven, B-3000 Leuven, Belgium and Institute for Materials Research, SCaPE, University of Leeds, Leeds LS2 9JT, UK s.h.doak@swansea.ac.uk.

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Effect of pH on QDot fluorescence intensity. Relative fluorescence intensity levels of 7.5 nM suspensions of amine- and carboxyl-QDs at various pH values (7.4, 5.5, and 4.5) as a function of time. Data are presented as mean ± SD.
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kfv002-F3: Effect of pH on QDot fluorescence intensity. Relative fluorescence intensity levels of 7.5 nM suspensions of amine- and carboxyl-QDs at various pH values (7.4, 5.5, and 4.5) as a function of time. Data are presented as mean ± SD.

Mentions: The 2 QD particles were incubated with media adjusted to different pH levels (7.4, 5.5, 4.5) for 1, 2, 3, 4, and 5 days and fluorescence intensity was analyzed. These experiments were conducted at 0, 2.5, 5, 7.5, 10, 15, and 20nM concentrations, however, the graph shown here presents only data for the 7.5 nM dose for the purpose of conciseness. Results revealed a sharp decline in fluorescence intensity with carboxyl-QDs starting at day 1 in all 3 pH media (Fig. 3A). Some decline in fluorescence was detected in the amine-QDs (Fig. 3B), however, this was not significant at any time point.FIG. 3.


Cell type-dependent changes in CdSe/ZnS quantum dot uptake and toxic endpoints.

Manshian BB, Soenen SJ, Al-Ali A, Brown A, Hondow N, Wills J, Jenkins GJ, Doak SH - Toxicol. Sci. (2015)

Effect of pH on QDot fluorescence intensity. Relative fluorescence intensity levels of 7.5 nM suspensions of amine- and carboxyl-QDs at various pH values (7.4, 5.5, and 4.5) as a function of time. Data are presented as mean ± SD.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4372665&req=5

kfv002-F3: Effect of pH on QDot fluorescence intensity. Relative fluorescence intensity levels of 7.5 nM suspensions of amine- and carboxyl-QDs at various pH values (7.4, 5.5, and 4.5) as a function of time. Data are presented as mean ± SD.
Mentions: The 2 QD particles were incubated with media adjusted to different pH levels (7.4, 5.5, 4.5) for 1, 2, 3, 4, and 5 days and fluorescence intensity was analyzed. These experiments were conducted at 0, 2.5, 5, 7.5, 10, 15, and 20nM concentrations, however, the graph shown here presents only data for the 7.5 nM dose for the purpose of conciseness. Results revealed a sharp decline in fluorescence intensity with carboxyl-QDs starting at day 1 in all 3 pH media (Fig. 3A). Some decline in fluorescence was detected in the amine-QDs (Fig. 3B), however, this was not significant at any time point.FIG. 3.

Bottom Line: Following thorough physicochemical characterization, cellular uptake, cytotoxicity, and gross chromosomal damage were measured.BEAS-2B cells demonstrated the highest level of QDs uptake yet displayed a strong resilience with minimal genotoxicity following exposure to these NPs.Thus, this study demonstrates that in addition to nanomaterial physicochemical characterization, a clear understanding of cell type-dependent variation in uptake coupled to the inherently different capacities of the cell types to cope with exposure to these exogenous materials are all required to predict genotoxicity.

View Article: PubMed Central - PubMed

Affiliation: *Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK, Department of Medicine, Biomedical NMR Unit-MoSAIC, KU Leuven, B-3000 Leuven, Belgium and Institute for Materials Research, SCaPE, University of Leeds, Leeds LS2 9JT, UK *Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK, Department of Medicine, Biomedical NMR Unit-MoSAIC, KU Leuven, B-3000 Leuven, Belgium and Institute for Materials Research, SCaPE, University of Leeds, Leeds LS2 9JT, UK s.h.doak@swansea.ac.uk.

Show MeSH
Related in: MedlinePlus