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Cell type-dependent changes in CdSe/ZnS quantum dot uptake and toxic endpoints.

Manshian BB, Soenen SJ, Al-Ali A, Brown A, Hondow N, Wills J, Jenkins GJ, Doak SH - Toxicol. Sci. (2015)

Bottom Line: Following thorough physicochemical characterization, cellular uptake, cytotoxicity, and gross chromosomal damage were measured.BEAS-2B cells demonstrated the highest level of QDs uptake yet displayed a strong resilience with minimal genotoxicity following exposure to these NPs.Thus, this study demonstrates that in addition to nanomaterial physicochemical characterization, a clear understanding of cell type-dependent variation in uptake coupled to the inherently different capacities of the cell types to cope with exposure to these exogenous materials are all required to predict genotoxicity.

View Article: PubMed Central - PubMed

Affiliation: *Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK, Department of Medicine, Biomedical NMR Unit-MoSAIC, KU Leuven, B-3000 Leuven, Belgium and Institute for Materials Research, SCaPE, University of Leeds, Leeds LS2 9JT, UK *Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK, Department of Medicine, Biomedical NMR Unit-MoSAIC, KU Leuven, B-3000 Leuven, Belgium and Institute for Materials Research, SCaPE, University of Leeds, Leeds LS2 9JT, UK s.h.doak@swansea.ac.uk.

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High angle annular dark field (HAADF) scanning transmission electron microscope (STEM) images confirming QDs uptake. (A) Carboxyl-QDs into TK6 cells accompanied with (B) higher magnification images of particles plus (C) assertion of particle composition by BF TEM EDX spectroscopy. No amine-QDs could be detected in TK6 cells using this technique (in line with ImageStream analysis). (D, E, F) Carboxyl- and (G, H, I) amine-QDs in BEAS-2B cells and (J, K, L) carboxyl and (M, N, O) amine-QDs uptake into HFF-1 cells.
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kfv002-F2: High angle annular dark field (HAADF) scanning transmission electron microscope (STEM) images confirming QDs uptake. (A) Carboxyl-QDs into TK6 cells accompanied with (B) higher magnification images of particles plus (C) assertion of particle composition by BF TEM EDX spectroscopy. No amine-QDs could be detected in TK6 cells using this technique (in line with ImageStream analysis). (D, E, F) Carboxyl- and (G, H, I) amine-QDs in BEAS-2B cells and (J, K, L) carboxyl and (M, N, O) amine-QDs uptake into HFF-1 cells.

Mentions: Defining ultimate subcellular localization of NPs inside cells can only truly be achieved by TEM and thus, this technique was subsequently employed to ascertain the positioning of QDs inside the test cells. TEM images of BEAS-2B, TK6, and HFF-1 cells revealed the presence of carboxyl-QDs in all 3 cell types (Figs. 2A, 2D, and 2J). Amine-QDs were also identified within BEAS-2B (Fig. 2G) and HFF-1 cells (Fig. 2M). With respect to the carboxyl- and amine-QDs, in some instances (eg, Fig. 2M), large collections of QDs could be identified at low magnifications, however in all cases higher magnification imaging and elemental spectroscopy were undertaken to both confirm the presence of the QDs and also to determine the intracellular location (Figs. 2B, 2E, 2H, 2K, and 2N). The QDs could be found either free in the cytoplasmic space or localized in intracellular vesicles which appeared to be endosomes or lysosomes. TEM images suggested amine-QDs were present in larger agglomerates in vesicles within HFF-1 cells (Fig. 2N) compared with those in the BEAS-2B cells (Fig. 2H). Similarly, more carboxyl-QDs were detected in BEAS-2B cells (Fig. 2E) followed by TK6 (Fig. 2B) and HFF-1 cells (Fig. 2K), respectively, which correlates with the ImageStream data presented in Figure 1.FIG. 2.


Cell type-dependent changes in CdSe/ZnS quantum dot uptake and toxic endpoints.

Manshian BB, Soenen SJ, Al-Ali A, Brown A, Hondow N, Wills J, Jenkins GJ, Doak SH - Toxicol. Sci. (2015)

High angle annular dark field (HAADF) scanning transmission electron microscope (STEM) images confirming QDs uptake. (A) Carboxyl-QDs into TK6 cells accompanied with (B) higher magnification images of particles plus (C) assertion of particle composition by BF TEM EDX spectroscopy. No amine-QDs could be detected in TK6 cells using this technique (in line with ImageStream analysis). (D, E, F) Carboxyl- and (G, H, I) amine-QDs in BEAS-2B cells and (J, K, L) carboxyl and (M, N, O) amine-QDs uptake into HFF-1 cells.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4372665&req=5

kfv002-F2: High angle annular dark field (HAADF) scanning transmission electron microscope (STEM) images confirming QDs uptake. (A) Carboxyl-QDs into TK6 cells accompanied with (B) higher magnification images of particles plus (C) assertion of particle composition by BF TEM EDX spectroscopy. No amine-QDs could be detected in TK6 cells using this technique (in line with ImageStream analysis). (D, E, F) Carboxyl- and (G, H, I) amine-QDs in BEAS-2B cells and (J, K, L) carboxyl and (M, N, O) amine-QDs uptake into HFF-1 cells.
Mentions: Defining ultimate subcellular localization of NPs inside cells can only truly be achieved by TEM and thus, this technique was subsequently employed to ascertain the positioning of QDs inside the test cells. TEM images of BEAS-2B, TK6, and HFF-1 cells revealed the presence of carboxyl-QDs in all 3 cell types (Figs. 2A, 2D, and 2J). Amine-QDs were also identified within BEAS-2B (Fig. 2G) and HFF-1 cells (Fig. 2M). With respect to the carboxyl- and amine-QDs, in some instances (eg, Fig. 2M), large collections of QDs could be identified at low magnifications, however in all cases higher magnification imaging and elemental spectroscopy were undertaken to both confirm the presence of the QDs and also to determine the intracellular location (Figs. 2B, 2E, 2H, 2K, and 2N). The QDs could be found either free in the cytoplasmic space or localized in intracellular vesicles which appeared to be endosomes or lysosomes. TEM images suggested amine-QDs were present in larger agglomerates in vesicles within HFF-1 cells (Fig. 2N) compared with those in the BEAS-2B cells (Fig. 2H). Similarly, more carboxyl-QDs were detected in BEAS-2B cells (Fig. 2E) followed by TK6 (Fig. 2B) and HFF-1 cells (Fig. 2K), respectively, which correlates with the ImageStream data presented in Figure 1.FIG. 2.

Bottom Line: Following thorough physicochemical characterization, cellular uptake, cytotoxicity, and gross chromosomal damage were measured.BEAS-2B cells demonstrated the highest level of QDs uptake yet displayed a strong resilience with minimal genotoxicity following exposure to these NPs.Thus, this study demonstrates that in addition to nanomaterial physicochemical characterization, a clear understanding of cell type-dependent variation in uptake coupled to the inherently different capacities of the cell types to cope with exposure to these exogenous materials are all required to predict genotoxicity.

View Article: PubMed Central - PubMed

Affiliation: *Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK, Department of Medicine, Biomedical NMR Unit-MoSAIC, KU Leuven, B-3000 Leuven, Belgium and Institute for Materials Research, SCaPE, University of Leeds, Leeds LS2 9JT, UK *Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK, Department of Medicine, Biomedical NMR Unit-MoSAIC, KU Leuven, B-3000 Leuven, Belgium and Institute for Materials Research, SCaPE, University of Leeds, Leeds LS2 9JT, UK s.h.doak@swansea.ac.uk.

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Related in: MedlinePlus