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Diversity of Cryptosporidium in brush-tailed rock-wallabies (Petrogale penicillata) managed within a species recovery programme.

Vermeulen ET, Ashworth DL, Eldridge MD, Power ML - Int J Parasitol Parasites Wildl (2015)

Bottom Line: No significant difference was found in Cryptosporidium detection between each of the three population categories.Cryptosporidium meleagridis has not been previously reported in marsupials and hence the pathogenicity of this species to brush-tailed rock-wallabies is unknown.Based on these findings, we recommend further study into Cryptosporidium in animals undergoing conservation management, as well as surveying wild animals in release areas, to further understand the diversity and epidemiology of this parasite in threatened wildlife.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Macquarie University, North Ryde, NSW, 2109, Australia.

ABSTRACT
Host-parasite relationships are likely to be impacted by conservation management practices, potentially increasing the susceptibility of wildlife to emerging disease. Cryptosporidium, a parasitic protozoan genus comprising host-adapted and host-specific species, was used as an indicator of parasite movement between populations of a threatened marsupial, the brush-tailed rock-wallaby (Petrogale penicillata). PCR screening of faecal samples (n = 324) from seven wallaby populations across New South Wales, identified Cryptosporidium in 7.1% of samples. The sampled populations were characterised as captive, supplemented and wild populations. No significant difference was found in Cryptosporidium detection between each of the three population categories. The positive samples, detected using 18S rRNA screening, were amplified using the actin and gp60 loci. Multi-locus sequence analysis revealed the presence of Cryptosporidium fayeri, a marsupial-specific species, and C. meleagridis, which has a broad host range, in samples from the three population categories. Cryptosporidium meleagridis has not been previously reported in marsupials and hence the pathogenicity of this species to brush-tailed rock-wallabies is unknown. Based on these findings, we recommend further study into Cryptosporidium in animals undergoing conservation management, as well as surveying wild animals in release areas, to further understand the diversity and epidemiology of this parasite in threatened wildlife.

No MeSH data available.


Samples were identified within a phylogenetic framework with the tree constructed using neighbour-joining with bootstrap test (1,000 replicates, displayed at nodes) using the 18S rRNA locus (878 bp). KV denotes Kangaroo Valley.
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f0015: Samples were identified within a phylogenetic framework with the tree constructed using neighbour-joining with bootstrap test (1,000 replicates, displayed at nodes) using the 18S rRNA locus (878 bp). KV denotes Kangaroo Valley.

Mentions: From the initial positive samples (n = 23), 20 samples yielded sequence data for the larger 18S rRNA fragment (825 bp), which was used to generate a phylogeny (Fig. 1). Four samples from supplemented sites and three samples from wild sites clustered with the C. parvum and C. hominis. Three samples from supplemented sites, one from a captive bred site and one from a wild site grouped with the marsupial-specific species C. fayeri and C. macropodum. A further four samples from a wild site and one from a captive-bred site grouped with C. meleagridis. Two samples from a wild site were grouped with C. ubiquitum.


Diversity of Cryptosporidium in brush-tailed rock-wallabies (Petrogale penicillata) managed within a species recovery programme.

Vermeulen ET, Ashworth DL, Eldridge MD, Power ML - Int J Parasitol Parasites Wildl (2015)

Samples were identified within a phylogenetic framework with the tree constructed using neighbour-joining with bootstrap test (1,000 replicates, displayed at nodes) using the 18S rRNA locus (878 bp). KV denotes Kangaroo Valley.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4372656&req=5

f0015: Samples were identified within a phylogenetic framework with the tree constructed using neighbour-joining with bootstrap test (1,000 replicates, displayed at nodes) using the 18S rRNA locus (878 bp). KV denotes Kangaroo Valley.
Mentions: From the initial positive samples (n = 23), 20 samples yielded sequence data for the larger 18S rRNA fragment (825 bp), which was used to generate a phylogeny (Fig. 1). Four samples from supplemented sites and three samples from wild sites clustered with the C. parvum and C. hominis. Three samples from supplemented sites, one from a captive bred site and one from a wild site grouped with the marsupial-specific species C. fayeri and C. macropodum. A further four samples from a wild site and one from a captive-bred site grouped with C. meleagridis. Two samples from a wild site were grouped with C. ubiquitum.

Bottom Line: No significant difference was found in Cryptosporidium detection between each of the three population categories.Cryptosporidium meleagridis has not been previously reported in marsupials and hence the pathogenicity of this species to brush-tailed rock-wallabies is unknown.Based on these findings, we recommend further study into Cryptosporidium in animals undergoing conservation management, as well as surveying wild animals in release areas, to further understand the diversity and epidemiology of this parasite in threatened wildlife.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Macquarie University, North Ryde, NSW, 2109, Australia.

ABSTRACT
Host-parasite relationships are likely to be impacted by conservation management practices, potentially increasing the susceptibility of wildlife to emerging disease. Cryptosporidium, a parasitic protozoan genus comprising host-adapted and host-specific species, was used as an indicator of parasite movement between populations of a threatened marsupial, the brush-tailed rock-wallaby (Petrogale penicillata). PCR screening of faecal samples (n = 324) from seven wallaby populations across New South Wales, identified Cryptosporidium in 7.1% of samples. The sampled populations were characterised as captive, supplemented and wild populations. No significant difference was found in Cryptosporidium detection between each of the three population categories. The positive samples, detected using 18S rRNA screening, were amplified using the actin and gp60 loci. Multi-locus sequence analysis revealed the presence of Cryptosporidium fayeri, a marsupial-specific species, and C. meleagridis, which has a broad host range, in samples from the three population categories. Cryptosporidium meleagridis has not been previously reported in marsupials and hence the pathogenicity of this species to brush-tailed rock-wallabies is unknown. Based on these findings, we recommend further study into Cryptosporidium in animals undergoing conservation management, as well as surveying wild animals in release areas, to further understand the diversity and epidemiology of this parasite in threatened wildlife.

No MeSH data available.