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Circulating 20S proteasome is independently associated with abdominal muscle mass in hemodialysis patients.

Fukasawa H, Kaneko M, Niwa H, Matsuyama T, Yasuda H, Kumagai H, Furuya R - PLoS ONE (2015)

Bottom Line: Multiple regression analyses showed that 20S proteasome was a significant independent predictor of abdominal muscle area (P < 0.05).In conclusion, plasma 20S proteasome concentrations were independently associated with abdominal muscle mass in hemodialysis patients.Our findings indicate a relationship between circulating 20S proteasomes and muscle metabolism in these patients.

View Article: PubMed Central - PubMed

Affiliation: Renal Division, Department of Internal Medicine, Iwata City Hospital, Iwata, Shizuoka, Japan.

ABSTRACT

Unlabelled: Protein-energy wasting is highly prevalent in hemodialysis patients, and it contributes to patient morbidity and mortality. The ubiquitin-proteasome system is the major pathway for intracellular protein degradation and it is involved in the regulation of basic cellular processes. However, the role of this system in the determination of nutritional status is largely unknown. To examine a relationship between protein-energy wasting and the ubiquitin-proteasome system, a cross-sectional study of 76 hemodialysis patients was performed. Plasma concentrations of 20S proteasome were studied to evaluate its association with muscle and fat mass, which were investigated by abdominal muscle and fat areas measured using computed tomography and by creatinine production estimated using the creatinine kinetic model. Plasma 20S proteasome concentrations significantly and negatively correlated with abdominal muscle areas and creatinine production (rho = -0.263, P < 0.05 and rho = -0.241, P < 0.05, respectively), but not abdominal subcutaneous and visceral fat areas. Multiple regression analyses showed that 20S proteasome was a significant independent predictor of abdominal muscle area (P < 0.05). In conclusion, plasma 20S proteasome concentrations were independently associated with abdominal muscle mass in hemodialysis patients. Our findings indicate a relationship between circulating 20S proteasomes and muscle metabolism in these patients.

Trial registration: UMIN Clinical Trials Registry UMIN000012341.

No MeSH data available.


Related in: MedlinePlus

Western blot analysis of 20S proteasome in 11 patients.Bands of the 20S proteasome are detected (arrow head). Laddered bands were also present under the specific bands (asterisk).
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pone.0121352.g001: Western blot analysis of 20S proteasome in 11 patients.Bands of the 20S proteasome are detected (arrow head). Laddered bands were also present under the specific bands (asterisk).

Mentions: Plasma levels of 20S proteasome measured with ELISA and Western blot analysis were significantly correlated each other (P < 0.05), but the ELISA values were slightly higher than Western blot analysis (1.34 ± 1.12 μg/mL and 1.33 ± 0.53 μg/mL, respectively, Table 1). Two patients demonstrated extremely high 20S proteasome levels when plasma samples were measured with ELISA (110.90 μg/mL and 70.10 μg/mL). Furthermore, the laddered bands, which might be included as levels when the samples were measured with ELISA, were present under the specific bands of 20S proteasome (Fig. 1). Therefore, we used the data measured with Western blot analysis in subsequent analyses.


Circulating 20S proteasome is independently associated with abdominal muscle mass in hemodialysis patients.

Fukasawa H, Kaneko M, Niwa H, Matsuyama T, Yasuda H, Kumagai H, Furuya R - PLoS ONE (2015)

Western blot analysis of 20S proteasome in 11 patients.Bands of the 20S proteasome are detected (arrow head). Laddered bands were also present under the specific bands (asterisk).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4372611&req=5

pone.0121352.g001: Western blot analysis of 20S proteasome in 11 patients.Bands of the 20S proteasome are detected (arrow head). Laddered bands were also present under the specific bands (asterisk).
Mentions: Plasma levels of 20S proteasome measured with ELISA and Western blot analysis were significantly correlated each other (P < 0.05), but the ELISA values were slightly higher than Western blot analysis (1.34 ± 1.12 μg/mL and 1.33 ± 0.53 μg/mL, respectively, Table 1). Two patients demonstrated extremely high 20S proteasome levels when plasma samples were measured with ELISA (110.90 μg/mL and 70.10 μg/mL). Furthermore, the laddered bands, which might be included as levels when the samples were measured with ELISA, were present under the specific bands of 20S proteasome (Fig. 1). Therefore, we used the data measured with Western blot analysis in subsequent analyses.

Bottom Line: Multiple regression analyses showed that 20S proteasome was a significant independent predictor of abdominal muscle area (P < 0.05).In conclusion, plasma 20S proteasome concentrations were independently associated with abdominal muscle mass in hemodialysis patients.Our findings indicate a relationship between circulating 20S proteasomes and muscle metabolism in these patients.

View Article: PubMed Central - PubMed

Affiliation: Renal Division, Department of Internal Medicine, Iwata City Hospital, Iwata, Shizuoka, Japan.

ABSTRACT

Unlabelled: Protein-energy wasting is highly prevalent in hemodialysis patients, and it contributes to patient morbidity and mortality. The ubiquitin-proteasome system is the major pathway for intracellular protein degradation and it is involved in the regulation of basic cellular processes. However, the role of this system in the determination of nutritional status is largely unknown. To examine a relationship between protein-energy wasting and the ubiquitin-proteasome system, a cross-sectional study of 76 hemodialysis patients was performed. Plasma concentrations of 20S proteasome were studied to evaluate its association with muscle and fat mass, which were investigated by abdominal muscle and fat areas measured using computed tomography and by creatinine production estimated using the creatinine kinetic model. Plasma 20S proteasome concentrations significantly and negatively correlated with abdominal muscle areas and creatinine production (rho = -0.263, P < 0.05 and rho = -0.241, P < 0.05, respectively), but not abdominal subcutaneous and visceral fat areas. Multiple regression analyses showed that 20S proteasome was a significant independent predictor of abdominal muscle area (P < 0.05). In conclusion, plasma 20S proteasome concentrations were independently associated with abdominal muscle mass in hemodialysis patients. Our findings indicate a relationship between circulating 20S proteasomes and muscle metabolism in these patients.

Trial registration: UMIN Clinical Trials Registry UMIN000012341.

No MeSH data available.


Related in: MedlinePlus